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Microbiology Exam 2 Microbial Evolution and Systematics
N/A
54
Biology
Undergraduate 3
11/25/2012

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Term
How old is the earth?
Definition
4.5 byo
Term
Evidence for microbial life?
Definition
stromatolites
Term
Stromatolites
Definition
fossilized microbial mats
layers of filamentous prokaryotes
Term
How old are the oldest stromatolites?
Definition
3.5 byo
Term
Surface Origin Hypothesis
Definition
The first membrane-enclosed, self-replicating cells arose out of primordial soup rich in organic and inorganic compounds in ponds on Earth’s surface
Term
What argue against the surface origin hypothesis?
Definition
Dramatic temperature fluctuations and mixing from meteor impacts, dust clouds, and storms argue against this hypothesis
Term
Sub surface origin hypothesis
Definition
Life originated at hydrothermal springs on ocean floor
Term
What are the two hypotheses of the origin of life?
Definition
surface and sub surface
Term
What two things argue for the sub surface hypothesis
Definition
Conditions would have been more stable
Steady and abundant supply of energy (e.g., H2 and H2S) may have been available at these sites
Term
What is the most likely self-replicating entity?
Definition
RNA
Term
How is self replicating entity possible?
Definition
RNAs can perform different catalytic functions
Term
RNA may have been enclosed in a
Definition
lipoprotein vesicle
Term
What replace RNA as catalysts?
Definition
protein
Term
What replaces coding functions of RNA?
Definition
DNA
Term
How did anoxic bacteria obtain carbon?
Definition
CO2
Term
How did anoxic bacteria obtain energy?
Definition
H2 most likely by reacting H2S with FeS
Term
What first created oxygen?
Definition
cyanobacteria
Term
When did cyanobacteria first appear?
Definition
2.7 bya
Term
What did cyanobacteria develope?
Definition
A photosystem that could use H2O instead of H2S, which generates O2
Term
Why could atmospheric oxygen not accumulate?
Definition
because there were still a lot of reduced material present
Term
O2 oxidized Fe2 (ferrous) into
Definition
fe3 ferric
Term
Ferric accumulates in
Definition
layers
Term
After Fe2 was consumed,
Definition
atmospheric oxygen could accumulate
Term
Oxygen was available as an _________ which resulted in more _______
Definition
electron acceptor, energy
Term
Atmospheric oxygen resulted in the formation of
Definition
an ozone layer
Term
An ozone layer meant
Definition
there were more places availbale for colonization because there was less UV
Term
Oxygen spurred evolution of
Definition
organelle containing eukaryotic microorganisms
Term
Unicellular eukaryotes arose on earth when?
Definition
2 bya
Term
Three important things in the origin of eukaryotes
Definition
-rise in oxygen
-invention of respiratory metabolism in bacteria
-development of enzymes like superoxide dismutase
Term
Endosymbiosis
Definition
Aerobic bacteria became established in a primitive eukaryote
Term
According to endosymbiosis mitochondria and chloroplasts of modern-day eukaryotes arose from
Definition
stable incorporation into another type of cell
A chemoorganotroph, which carried out facultatively aerobic metabolism
A cyanobacteria, which carried out oxygenic photosynthesis.
Term
Evidence for endosymbiosis.
Chloroplast and mitochondrial ______ are similar to bacteria
Definition
RNA sequences
Term
Evidence for endosymbiosis.
Eukaryotic organellar ribosomes are _____ that inhibit bacteria
Definition
inhibited by antibiotics
Term
Evidence for endosymbiosis
Mitochondria and chloroplasts have
Definition
small circular pieces of DNA
Term
Formation of the eukaryotic cell hypothesis one.
Definition
Eukaryotes began as a nucleas bearing lineage that later aquired mitochondria and chloroplasts through endosymbiosis. The nucleus arose spontaneously, in response to the increasing genome size
Term
Formation of the eukaryotic cell, hypthesis two (hydrogen hypothesis)
Definition
A eukaryotic cell arose from an intracellular relationship between a symbiont and an H2 consuming host.
Term
Eukaryote formation, hypothesis two.
What is the symbiont?
Definition
A O2 consuming, H producing bacteria.
Ancestor to a mitochondria
Term
Eukaryote formation, hypothesis two.
When did the nucleus arise?
When were chloroplasts aquired?
Definition
After a stable association
later by endosymbiosis
Term
Chimera
Definition
a cell made up of both parts of bacteria and archae
Term
Example of a chimera
Definition
eukaryotes
Term
What in eukaryotes are more similar to archae?
Definition
transcription and translation
Term
What in eukaryotes are more similar to bacteria?
Definition
lipids
Term
phylogeny
Definition
evolutionary history of a group of organisms
Term
How is phylogeny inferred indirectly?
Definition
nucleotide sequence data
Term
Evolutionary chronometers
Definition
measures evolutionary change through certain genes and protiens
Term
Four features of genes employed in phylogenic analysis
Definition
1. Universally distributed
2. Functionally constant
3. sequence sufficiently (change over time is extremely slow)
4. of adequate length
Term
The most widely used are genes encoding _______ (prokaryotes) and _______ (eukaryotes)
Definition
16s rRNA and 18s rRNA
Term
What satisfies all criteria as a gene employed in phylogentic analysis
Definition
rRNA
Term
What are the three rRNA molecules
Definition
5S, 16S (18S in euk.), 23S
Term
What kind of sequencing is used on rRNA
Definition
SSU
Term
Who pioneered rRNA sequencing?
Definition
Carl Woesse
Term
What is the Ribosomal Database project?
Definition
A large collection of rRNA sequences
Currently contains >1.3 million sequences
Provides a variety of analytical programs
Term
What does BLAST stand for
Definition
Basic Local Alignment Search Tool
Term
What is BLAST and what does it do?
Definition
Web-based tool of the National Institutes of Health
Aligns query sequences with those in GenBank database
Helpful in identifying gene sequences
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