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Microbiology Competency
20 Organisms on MLS Clinical Microbiology Competency Exam
20
Microbiology
Undergraduate 4
04/06/2017

Additional Microbiology Flashcards

 


 

Cards

Term
Staphylococcus aureus
Definition
GPC in clusters; looks like grapes
Large, creamy white colonies; buttery
"old sock" smell
Beta hemolysis on MSA
Catalase + and Coagulae +
Term
Staphylococcus epidermidis
Definition
GPC in clusters
Small-Medium, creamy white colonies
No hemolysis on SBA
Catalase + , Coagulase Neg, Oxidase Neg
Term
Micrococcus sp.
Definition
GPC
Yellow pigment on SBA
Catalase +, Coagulase Neg, Oxidase +
Term
Streptococcus pneumoniae
Definition
GPC diplococci or short chains
Penny edge and alpha hemolysis on SBA
Catalase Neg, P disk "S", Bile soluble
Term
Streptococcus pyogenes
Definition
GPC and small colonies
Wide zone of beta hemolysis on SBA
Catalase Neg, A disk "S", PYR +
Term
Streptococcus agalactiae
Definition
GPC pairs or chains
Medium, flat, translucent colonies
Narrow zone of beta hemolysis on SBA
Catalse Neg, CAMP +, Hippurate +
Term
Streptococcus anginosis (viridians)
Definition
GPC chains
Alpha hemolysis on SBA
Catalase Neg, PYR Neg, Bile esculin Neg
P disk "R"
Term
Enterococcus sp.
Definition
GNR elongated pairs or chains
y-hemolysis
Catalase Neg, Bile esculin +, 6.5% NaCl +
PYR +
Term
Candida sp.
Definition
Yeast GP Budding or pseudohyphae
Small, white, dull, "heaped" or convex
Projections or "feet" at edge of colony
Germ tube +, urease Neg, Growth at 42C
Term
Escherichia coli
Definition
GNR
Dry, flat colonies
Pink on MAC w/ precipitate; Green on EMB
Lactose +, Oxidase Neg, Motile
IMViC +, +, Neg, Neg
Term
Klebsiella pneumoniae
Definition
GNR
Large, mucoid, pink colonies on MAC
Oxidase Neg, Nonmotile, H2S Neg
Term
Proteus mirabilis
Definition
GNR
Swarming on SBA and burnt chocolate smell
Indole Neg, Ornithine +, Urea +
Term
Proteus vulgaris
Definition
GNR
Swarming on SBA
Indole +, Ornithine Neg, Urea +
Term
Pseudomonas aeruginosa
Definition
GN bacillus/coccobacillus
Metallic sheen and green pigment on MAC
Growth at 42C w/ fruity, grape-like smell
Oxidase +, Citrate +, Motile, Assachrolytic
Term
Moraxella sp.
Definition
GN intracellular diplococci
Smooth, opaque, gray/white on SBA and CHOC
Hockey puck and wagon wheel
Oxidase +, Catalase +, DNase +, assachrolytic
Can grow in 28C
Term
Haemophilus sp.
Definition
GNR pleomorhic
Tan, translucent, smooth, moist, convex
CHOC with Factor X and V
Satellism possible
Catalase +, Oxidase +, Nitrate Reduction +
Nonmotile
Mousy or bleach-like smell
Term
Neisseria gonorrhoeae
Definition
GN intra/extracellular diplococci
Small, raised, gray/tan, translucent
Needs iron, CHOC or selective medium
Thayer-Martin or Martin-Lewis medium
Capnophilic
Uses glucose only
Term
Neisseria meningitides
Definition
GN intra/extracellular diplococci
Medium, gray, green tinge, convex, mucoid
Needs iron, CHOC or selective medium
Capnophilic
Uses glucose and maltose
Term
Salmonella sp.
Definition
GNR
Clear, colorless colonies
Black center (H2S) on HE or XLD
Non-lactose fermenting on MAC
H2S +, GLU +, Nitrate Reduction +
Indole Neg, VP Neg, PAD Neg, Motile
Term
Shigella sp.
Definition
GNR
Clear, fragile, non-lactose on MAC
H2S Neg, GLU Neg, Lysine Neg, nonmotile
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