Shared Flashcard Set

Details

Microbiology C-1
exam 1 review
49
Biology
Undergraduate 1
01/07/2009

Additional Biology Flashcards

 


 

Cards

Term
Name the prokaryotic groups in the microbial world
Definition
All bacteria and archea
Term
Theory of spontaneous generation
Definition
The discredited theory that organisms can arise from non-living matter
Term
List five beneficial applications of bateria
Definition

1.  protection against disease

 

2.  competetion with disease causing bacteria

 

3.  development of the intestine

 

4.  process foodstuffs that the body can't digest

 

5.  antibiotics

Term
Define prokaryote
Definition

prokaryote-single-celled organism that does not contain a membrane-bound nucleus nor any other membrane-bound organelles

 

prokaryotic cell-cell characterized by lack of a nuclear membrane and the absence of membrane bound organelles

Term

The prokaryotic members of the microbial world include

1.  algae      2.  fungi    3.  prions    4.  bacteria

     

5.  archeaea

 

a) 1, 2     b) 2,3     c) 4,5     d) 1,5

Definition
d
Term
Define eukaryote
Definition

Organism composed of one or more eukaryotic cells

 

 

eukaryotic cell-Complex cell type differing from a prokaryotic cell mainly in having a nuclear membrane

Term
What is the tiniest microbe and what part of the body does it affect?
Definition
prions/ the brain and CNS
Term

The archaea

 

1.  are microscopic

 

2.  are commonly found in extreme environments

 

3.  contain peptidoglycan

 

4.  contain mitochondria

 

5.  are most commonly foundly found in soil

 

a)  1,2  b) 2,3  c) 3,4  d) 4,5  e) 1,5

Definition
A
Term
What is the difference between bacteria and viruses?
Definition

bacteria=a domain of prokaryotes

 

virus=Acellular or non-living agent composed of nucleic acid surrounded by a protein coat

Term
What is the main role of fungi?  Is it a eukaryote or a prokaryote?
Definition

 

recycling and decomposing/eukaryote

Term

The agent that contains no nucleic acid is a

 

a) virus     b) prion     c) viroid    d) bacterium

 

e) fungus

Definition
b
Term
Where is the habitat for bacteria?
Definition
most places
Term
Where is the habitat for bacteria?
Definition
most places
Term

Prokaryotes do not have

 

a) cell walls     b) flagella     c) a nuclear membrane

 

d) genetic shapes     e) genetic information

Definition
c
Term
What is the habitat for viruses? Are they cells?
Definition
host organisms/ no
Term

Which of the following are eukarotes?

 

1. algae      2. viruses    3.  bacteria     4.  prions   

 

5.  protozoa

 

a) 1,2     b) 2,3     c) 3,4     d) 4,5     e) 1,5

Definition
e
Term
Define the theory of spontaneous generation
Definition
Discredited theory that organisms can arise from non-living matter
Term
What is the germ theory of disease?
Definition
A theory that proposes that microorganisms are the cause of many diseases
Term
What is the system of binomial nomenclature?
Definition
This refers to a two word naming systerm
Term
What is the first word in the name in nonclemenclature called?  Is it capitalized?
Definition
genus/yes
Term
What is the second letter in a word called?  Is it capitalized?
Definition
species/no
Term
What is the genus/species and species for Escherichia coli or E. coli?
Definition

Escherichia=genus

E.

 

coli=species

Term
What are covalent, ionic, and hydrogen bonds? 
Definition

covalent= A strong chemical bond formed by the sharing of electrons between atoms

 

ionic= A bond formed by the attraction of positively charged atoms or molecules to negatively charged ones

 

hydrogen= A weak bond resulting from the attraction between a positively charged atoms in one compound and a negatively charged atom in another compound

Term
What is the difference between an acid, a base, and a buffer?
Definition

acid=pH<7

 

base=pH>7

 

buffer= prevents drastic shifts in pH, which would be deleterious to growth.  These keep pH near neutral

Term
Describe the classes of organic molecules: carbohydrates, lipids, proteins and nucleic acids (monomers and polymers)
Definition

All macromolecules are polymers

 

carbohydrate- characterized by a large number of -OH groups and containing principally carbon, hydrogen, and oxygen in a ratio of 1:2:1

 

lipids- a heterogenous group of organic molecules characterized by being insoluble in water, but soluble soluble in organic solvents such as ether, chloroform, and benzene

 

nucleic acids- a macromolecule consisting of chains of nucleotide subunits to form either DNA or RNA

Term
What is protein denaturation?
Definition

When proteins encounter different conditions such as high tempertaure, highor low pH or certain solvents, bonds within the protein are broken and its shape changes.  The protein becomes denatured and no longer functions

Term

The strongest bonds between two atoms in solution are

 

a) covalent     b) ionic      c) hydrogen bonds   

 

d) hydrophobic interaction 

 

 

 

Definition
a
Term

The primary structure of a protein relates to its

 

a) sequence of amino acids    b) length     c) shape

 

d) solubility    e) monosaccharides

Definition
a
Term
What is the difference between a simple, negative, endospore, Gram, and acid fast stain?
Definition

simple- employs a basic dye to impart a color to a cell.  It is easy to increase the contrast between otherwise colorless cells and a transparent background

 

negative- acid dyes used to stain backgrounds against which colorless cells can be seen.

 

endospore- a dormant cell that is resistant to destruction and staining

 

Gram- the most widely used procedure for staining bacteria

 

acid fast stains- A procedure used to stain a small group of organisms that don't readily take up stains

Term
Explain the different forms- coccus, bacillus, vibrio, spirillum, spirochete
Definition

coccus- most common bacteria

 

bacillus- common rod shaped bacteria

 

vibrio- bacteria that is a short curved rod

 

spirillum-a curved rod long enough to form spirals

 

spirochete- type of long helical cell wall and an axial filament

Term

Explain the arrangements- single, diplo, strepto etc.

Definition

diplo-two

 

strepto-multiple

Term
What is glycocalyx?
Definition
Slime layer produced by bacteria
Term
What is flagellum?
Definition
A long protein structure responsible for most types of bacterial motility.
Term
What is an axial filament?
Definition
Structure of motility found in spirochetes
Term
What is fimbriae?
Definition
Type of pilus that enable cells to attach to a specific surface.
Term
What is pili?
Definition
Pili are considerably shorter and thinner than flagella.  They have a string of protein subunits arranged helically. The functions of pili are different from flagella.  Many types of pili enable attachment of cells to specific surfaces. 
Term
What is a covalent bond?
Definition
When electrons are shared.  It is the strongest bond
Term
What is an adhesin molecule?
Definition
A protein that adheres by binding to a very specific molecule
Term
What is a peptidoglycan?
Definition
Macromolecule found only in bacteria that provides rigidity to the bacterial cell wall.
Term
What is the difference between polar and non-polar covalent bonds?
Definition

polar=unequal sharing

 

non polar=equal sharing

Term
What is teichoic acid?
Definition
Component of the Gram-positive cell wall, composed of chains of a common subunit, either ribitol phosphat to which various sugars and D-alanine are usually attached 
Term
What are the properties of water?
Definition

1.  high heat capacity

 

2.  doesn't evaporate very readily

 

3.  keeps its shape

Term
What does the outer membrane do?
Definition
This serves as a barrier to the passage of most molecules
Term

The theory that organsims can arise from non living matter is called

 

A) spontaneous generation

 

B) biogenesis theory

 

C) cell theory

 

D) the theory of evolution

Definition
A
Term

Which of the following experimental requirements was necessary for Pasteur to disprove spontaneous generation?

 

A)  providing a nutrient source that would support microbial growth

 

B)  providing air

 

C)  preventing airborne organisms from entering the flask

 

D)  heating the flask to kill any microorganisms that were initially present in the broth

 

E)  all of the above

Definition
E
Term

All of the following are true with regard to bacteria Except:

 

A)  They are single celled

 

B)  Most have a rigid cell wall made of cellulose

 

C)  They don't contain a true nucleus, that is they are prokaryotes

 

D)  They multiply by binary fission

 

E)  Many have flagella for movement

Definition
B
Term

Microbes that are very similar in shape, size and appearance to the bacteria, but able to live in environments of very high salt concentration and temperatures above boiling

 

A)  prokaryote     B)  eucharea     C)  archaea

 

D)  animalia     E)  protozoa

Definition
C
Term
What are the four main types of macromolecules? subunits?
Definition
proteins, polysaccharides, nucleic acids and lipids
Term
What are the four levels of protein?
Definition

1.  primary- The number and arrangement or sequence of amino acids in the polypeptide chain

 

2.  secondary- several different arrangements, a  helix or B sheets

 

3.  tertiary- chain folds back into a three dimensional shape 

 

4.  quaternary- proteins that consist of more than one polypeptide chain

 

 

 

 

Supporting users have an ad free experience!