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Microbiology Basics
Gram Positive
30
Bible Studies
12th Grade
05/12/2012

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Term
Encapsulated Bacteria
Definition
Streptococcus Pneumoniae
Klebsiella
Haemophilus Influenzae
Neisseria meningitidis
Salmonella
Group B strep

"Some killers have nice shiny bodies"
Term
Bacteria with unusual cell wall
Definition
Mycobacterium (mycolic acid)
Mycoplasma (sterol)
Term
Bacteria that do not gram stain well
Definition
Treponema (too thin)
Rickettsia (intracellular parasite)
Mycobacteria (high lipid content cell wall requires acid fast)
Mycoplasma (no cell wall)
Legionella
Chlamydia
Term
Microbe stains
Definition
Giemsa - chlamydia, borrelia, trypanosomes, plasmodium

PAS - glycogen mucopolysaccharides. tropheryma whippeli

Zhiehl-Neelsen - acid fast organisms

India Ink - Cryptococcal neoformans

Silver Stain - fungi, legionella
Term
Acute Phase Reactants
Definition
IL-1, IL-6, TNF-alpha
Term
Bugs with exotoxins:
TSST-1
Definition
Staph Aureus
Term
Bugs with exotoxins:
Erythrogenic toxin causing toxic shock/Scarlet fever
Definition
Streptococcus Pyogenes
Term
Bugs with exotoxins:
Inactivates elongation factor (EF-2)
Definition
Corynebacterium diphtheriae
Pseudomonas exotoxin A
Term
Bugs with exotoxins:
ADP robosylation of G protein stimulates adenylyl cyclase.

Pumps Cl- into gut
Definition
Vibrio cholerae
Term
Bugs with exotoxins:
Increases cAMP by inhibiting G-alpha1 receptors (inhibit the inhibitors)
Definition
Bordetella pertussis via diptheria toxin

Whooping cough
Term
Bugs with exotoxins:
Blocks the release of inhibitory neurotransmitters GABA (CNS) and glycine (PNS)
Definition
Clostridium Tetani

Lock Jaw

Inhibitsglycine release from RENSHAW cells in spinal cord.
Term
Bugs with exotoxins:
Blocks the release of acetylcholine. Found in canned food/honey
Definition
Clostridium Botulinum

Floppy baby
Causes anticholinergic symptoms, CNS paralysis.
Term
Bugs with exotoxins:
Alpha toxin causes gas gangrene. Phospholipase activity causes necrosis
Definition
Clostridium perfringens
via alpha toxin (lecithinase)

Double zone of hemolysis on blood agar.
Term
Bugs with exotoxins:
Releases edema factor. Spores from contaminated wool.
Definition
Bacillus anthracis

Lethal factor and edema (mediastinal widening). Transmitted via contaminated wool.

Can cause cutaneous and pulmonary anthrax
Term
Bugs with exotoxins:
Cleaves 60S ribosome
Definition
Shigella via shiga toxin
Term
Bugs with exotoxins:
Streptolysin O (a hemolysin), an antigen for ASO antibody.
Definition
Strep Pyogenes

Used to diagnose rheumatic fever
Term
Culture for:
H. Influenza
Definition
Chocolate agar
with factors V (NAD+) and factor X (hematin)
Term
Culture for:
N. gonorrhoeae
Definition
Thayer Martin (or VPN)

VPN = Vancomycin, polymyxin, nystatin)
Term
Culture for:
B. Pertussis
Definition
Bordet Gengou (potato) agar
Term
Culture for:
C. diptheriae
Definition
Tellurite Plate
Loffler's media
Term
Culture for:
M. tuberculosis
Definition
Lowenstein-Jensen agar
Term
Culture for:
Lactose-fermenting enterics
Definition
MacConkey's agar

E. Coli also grown on eosin-methylene blue agar (EMB)
Term
Culture for:
Legionella and yeast
Definition
Charcoal Yeast buffered with cysteine
Term
Culture for:
Fungi
Definition
Sabouraud's agar
Term
Bugs of exotoxins:
ADP ribosylation from gram negative bacteria
Definition
ETEC

Labile
Stabile
Term
Obligate anaerobes
Definition
Clostridium
Bacteroides
Actinomyces

anaerobes "Cant Breath Air"

Tx:
Metronidazole
Clindamycin
Term
Obligate aerobes
Definition
Nocardia
Pseudomonas
Mycobacterium tuberculosis
Bacillus anthracis
Term
Acquired from ingestion of unpasteurized milk/cheese and vaginal meats or by vaginal transmission at birth
Definition
Listeria monocytogenes

One of the most common neonatal infections with (ecoli and GBS). Causes meningitis.

Only gram positive bacteria with endotoxin.
Term
Anaerobic gram positive aerobe that forms yellow sulfur granules
Definition
Actinomyces israelii

Apart of normal oral flora
Causes abcesses that drain through tract of skin
Term
Bugs with IgA protease
Definition
S. pneumoniae
H. influenzae type B
Neisseria spp.
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