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Microbiology 205 genetics
Pima CC Armstrong
45
Biology
Undergraduate 2
10/05/2010

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Term
What is Genetics
Definition
How, when and why Genes are expressed
Term
What is the subdivision of the genome?
Definition
all genetic material is the Genome and its further divided into Chromosomes and plasmids and then genes and non coding regions
Term
what are the 4 nucleotides in DNA
Definition
Adenine, Thymine, Cytosine and Guanine
Term
what are the 4 nucleotides in RNA
Definition
Adenine, Urasil, Cytosine and Guanine
Term
What year was the structure of DNA discovered and who got credit?
Definition
1953 Francis Crick and James Watson
Term
2 enzymes responsible for the uncoiling of DNA
Definition
Topoisomerase and Gyrase
Term
Enzyme that unzips the unwound DNA
Definition
Helicase
Term
What is Semi-Conservative replication
Definition
1 parental strand + 1 new strand = daughter DNA
Term
What is the proofreading enzyme called
Definition
DNA polymerase
Term
The first step in going from genes to proteins is called (not the entire genome)
Definition
transcription
Term
What tells The RNA polymerase to stop
Definition
a specific region of DNA known as a terminator
Term
how many possible codons
Definition
64
Term
How many Amino Acids?
Definition
20 (for this class)
Term
how many non coding codons
Definition
3 stop codons
Term
Codon preference or usage bias mainly serves what function
Definition
relates to the speed of translation
Term
How many t-RNA molecules are there?
Definition
64 one for each codon
Term
when t-RNA has bound an amino acid what is it called?
Definition
charged
Term
what is the site of the t-RNA called that binds to a codon
Definition
anticodon
Term
the genetic makeup of an organism
Definition
Genotype - Anything an organism can make or do must be encoded for in the genotype
Term
the observable or measurable traits an organism has
Definition
Phenotype - For example, the ability to ferment lactose is a phenotype
Term
Missense mutation
Definition
occur when a change in one nucleotide results in a change of 1 amino acid
CTT to CTA
Term
Nonsense mutations
Definition
These mutations occur when a base change results in a nonsense codon, prematurely stopping protein synthesis
ATG to ATT (stop)
Term
Frameshift mutation
Definition
Occur when one nucleotide is either added or subtracted from the gene so
The message is completely changed
Term
Vertical gene transfer
Definition
genes passed from parent to offspring
Term
Horizontal gene transfer
Definition
genes passed from one organism to another
Term
Transformation
Definition
refers to the passing of DNA from one bacteria to another in the form of naked DNA
Term
Griffin’s experiment
Definition
killed mice by mixing non capsulated and dead capsulated bacteria and pulling out live capsulated bacteria. Naked DNA from dead decaying capsulated bacteria was taken up by live bacteria and they learned to make capsules
Term
Plasmids
Definition
are nothing more that small self replicating circular pieces of extra-chromosomal DNA
Term
Conjugative plasmids
Definition
carry genes for conjugation
Term
Dissimilation plasmids-
Definition
carry genes for unusual sugar and carbohydrate usage
Term
Resistance plasmids-
Definition
carry genes which encode antibiotic resistance
Term
Virulence plasmids-
Definition
carry genes which encode virulence traits
Term
Transduction
Definition
occurs when a phage (virus of bacteria) accidentally transfers bacterial DNA into another host bacteria
Term
Transposons
Definition
small fragments of DNA which can move around or transpose to different locations in the genome
Term
Repressor proteins
Definition
any protein which decrease or prevent gene transcription
This is typically done by impeding the RNA Polymerase
Term
Inducer proteins
Definition
any protein which promote or increase gene transcription
This is typically done by removing repressors or promoting RNA polymerase binding
Term
the control region
Definition
Binding site of repressors and inducers located upstream of the coding gene, and typically contains a regulatory gene, a promoter region and an operator region
Term
The promoter region
Definition
is where RNA polymerase binds to the DNA and initiates transcription
Term
The operator region
Definition
is where repressors bind DNA to block transcription
Term
The regulatory gene
Definition
is upstream of both the promoter and operator, and encodes the repressors and inducers
Term
Operons
Definition
are clusters of several genes which serve one function under control of a single regulatory region
Term
Biotechnology
Definition
Any use of life (cells, plants, microorganisms) to make a product
Term
Restriction enzymes
Definition
These recognize short sequences of DNA and cut reliable at the same place in that sequence.
DNA with blunt or sticky ends
Term
Transformation: (introducing a vector)
Definition
this is done with chemicals and temperature shock
Term
Electorporation
Definition
this technique uses electricity to make small holes or pores in the cell though which the plasmid can travel.
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