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MicroBio 202 Lab Final Exam
Dr. Keating
6
Biology
Undergraduate 3
11/22/2008

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Term
Selective medium
Definition
one that contains substances that areihibitory for many microorganisms, but allows the growth of some category or class of organisms. The inhibitory substance selectsfor growth of the desired type of orgamism(s).
Term
low pH favors ____ while inhibiting most _______
Definition
condition favors growth of fungi while inhibiting most bacteria
Term
the presence of high salt concenrations favors ____ while inhibiting most other bacterial species.
Definition
This environment favors the growth of Staphylcoccus species while inhibiting most otherbacterial species.
Term
What is the purpose of enrichment methods?
Definition
a method of selective plating - it gives the dsired species a significant competitive advantagethrough the production of a substrate that the species can use effectively. For example, lactose in the medium facors lactose fermenters. Lactic acid, on the other hand, favors Proprionibacterium species over other species that may be present in a mixed culture.
Term
EMB
Definition
a commonly used selective medium (eosin-methylene blue) agar. Selects for gram negative bacteria (which can grow in the presence of bile salts/the two dyes), or gram positive organisms of intestinal origin.
Term
What are two ways to select for types of organisms
Definition
using inhibitory substances (i.e. low pH, high salt) or enrichment methods (lactose, lactic acid)
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