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Microbial Metabolism
Test Two
75
Microbiology
Undergraduate 3
02/01/2013

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Term
Metabolism
Definition
all chemical reactions taking place within a cell
Term
Physiology
Definition
the study of life processes
Term
Anabolism 2 things
Definition
energy requiring, produces cell components
Term
Catabolism 2 things
Definition
energy yielding, produces waste products
Term
Is the cell an open or closed system?
Definition
open
Term
Microbial nutrition
Definition
supplying cells with the chemical constituents (nutrients) of monomers
Term
What are the 4 nutrients that the cells eat
Definition
macronutrients, micronutrients, essential nutrients, metabolite
Term
what percent of the cell is carbon?
Definition
50
Term
Heterotroph
Definition
carbon from organic carbon sources (eating from building blocks)
Term
Autotroph
Definition
carbon from CO2, energy from light or organic molecules
Term
What percent of the cell is nitrogen?
Definition
12
Term
ammonia
Definition
NH3
Term
nitrate
Definition
NO3-
Term
nitrogen
Definition
N2
Term
Nitrogen fixation
Definition
fill in card
Term
Where is a large source of oxygen?
Definition
water
Term
molecular oxygen is required for what
Definition
energetic needs and terminal
Term
Obligate aerobes
Definition
require oxygen
Term
Obligate anaerobes
Definition
poisoned by O2
Term
facultative anaerobes
Definition
can work either way
Term
What are some other macronutrients?
Definition
phosphorus, sulfur, potassium, magnesium, calcium, sodium, iron
Term
Micronutrients
Definition
are organic molecules required in small amounts
Term
Vitamins are what? Think physiology
Definition
coenzymes!
Term
What are 4 vitamins mentioned in lecture?
Definition
thiamine, biotin, pyridoxine, coalbumin
Term
Growth factor requirements: phototroph
Definition
derives all carbon requirements from the principle carbon source (glucose)
Term
Growth factor requirements: auxotroph
Definition
Requires one or more organic nutrients in addition to the principle carbon source. (can make all required C from media along, need one thing provided)
Term
Catalysis and enzymes control what?
Definition
the rate of the reaction
Term
What is activation energy?
Definition
amount of energy required to bring all molecules in a chemical reaction to the reactive state
Term
What does a catalyst do?
Definition
lowers the activation energy, increases rate of reaction and is unchanged
Term
What is an enzyme (general)
Definition
biological catalyst
Term
Exoenzyme
Definition
gets sent outside the cell to aid in absorption from the outside
Term
8 things about enzymes
Definition
proteins, catalyze a specific rxn, substrate, enzyme-substrate complex, active site, product, endoenzymes, exoenzymes
Term
What are the two requirements of catalysis?
Definition
bind correct substrate, position substrate relative to active site
Term
What is the structure of enzymes?
Definition
precise conformation, prosthetic groups, coenzymes
Term
What is a prosthetic group?
Definition
a larger group attached to an enzyme
Term
Is the enzyme chemically changed in a reaction?
Definition
no
Term
What is one way that the cell generates energy?
Definition
oxidation-reduction
Term
what is oxidation?
Definition
removal of electrons
Term
what is reduction?
Definition
addition of electrons
Term
what is a redox reaction?
Definition
e- donor and e- acceptor
Term
in a redox rxn, what e- is the energy source?
Definition
the e- donor (oxidation)
Term
What is dehydrogenation
Definition
removal of H
Term
What are the intermediates in electron transfers?
Definition
electron carriers
Term
What is the primary electron donor?
Definition
electron carriers
Term
What is the terminal electron acceptor?
Definition
electron carriers
Term
What kind of energy change is there with electron carrieres?
Definition
net
Term
What are the three stages of redox rxns?
Definition
removal of electrons from primary donor, transfer of electrons through one or a series of electron carriers, and addition of electrons to terminal acceptor
Term
how is energy stored?
Definition
as ATP
Term
What type of bonds are present in the storage of electrons?
Definition
high energy phosphate bonds
Term
ATP 4 points
Definition
- prime energy carrier
- two high energy P bonds
- formed during exergonic rxns
- drives endergonic rxns
Term
Types of Redox: Fermentation--are electron terminals added to aid the process?
Definition
No, absence of them
Term
Types of Redox: Fermentation - what is oxidation couples to?
Definition
reduction of substrate derived organic compound
Term
Types of Redox: Fermentation, what are the products and what is the net ATP production?
Definition
fermentation products, 2
Term
Types of Redox: Respiration - what is the process
Definition
O2, some other oxidant is terminal electron acceptor
Term
Types of Redox: Respiration: what is oxidation coupled to?
Definition
reduction of electron acceptor
Term
Types of Redox: Reduction - what does it form and what is the ATP production?
Definition
CO2 and 36 (proks) 38 (euks)
Term
respiration and electron transport systems control the transfer of ____
Definition
e-
Term
Respiration and electron transport systems: what is it?
Definition
membrane-associated electron carriers
Term
Respiration and electron transport systems: Functions
Definition
Accept, transfer electrons and conserve energy
Term
What generates proton motive force?
Definition
Respiration and electron transport systems
Term
What is the proton motive force?
Definition
electron transport carriers orientated in membrane
Term
What occurs across the membrane of the proton motive force?
Definition
separation of protons from electrons
Term
Where are protons transported in proton motive force?
Definition
outside of the cell
Term
Where are electrons transported in proton motive force?
Definition
through chain to the cell, where they pass to a final acceptor
Term
What does the proton motive force generate?
Definition
a pH gradient and an electrochemical potential
Term
What are the two major energy categories of microorganisms?
Definition
phototrophs and chemotrophs (chemotrophs break down into chemoorganotrophs and chemolithotrophs)
Term
How do phototrophs get energy?
Definition
photosynthesis
Term
How do chemotrophs get energy?
Definition
O2 (chemical energy)
Term
Chemotrophs: chemoorgaanotrophs energy requiremetns
Definition
organic molecules- carbon, etc
Term
Chemotrophs: chemolithotrophs energy requirement
Definition
need inorganic molecules (aid in creating the biosphere)
Term
Carbon: energy/carbon categories - photoautotroph
Definition
photosynthesis (fixes CO2)
Term
Carbon: energy/carbon categories - photoheterotroph
Definition
light but need preformed organic molecules
Term
Carbon: energy/carbon categories - chemoheterotroph (chemoorganoheterotroph
Definition
premade organic molecules and same molecules for building blocks
Term
Carbon: energy/carbon categories - chemoheterotroph (chemoorganoautotroph)
Definition
Get carbon from Carbon fixation
Term
Carbon: energy/carbon categories - chemolithoautotroph
Definition
*** listen to lecture and fill in
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