Technique to identify whereproteins bind to DNA. Mix proteins withDNA,add DNase, run on gel. Where proteins bind will show up as gaps in gel compared to no protein control.

Suicide Plasmid:

Tool used in genetic screens and engineering. Plasmid that does not replicate in recipient strain (not necessarily narrow host range plasmid)that is used as a delivery  mechanism for transposonsor other genetic material to be incorporated intogenome. Since suicide plasmids can be transferred butcan’t replicate, you can select for strains that incorporatetransposon (or other genetic material) into genomeby selecting for cells that incorporate antibioticresistance gene present in transposon into genome, because plasmid providing resistance will be diluted out of population over time.

Rolling Circle Replication:

During plasmid replication for conjugation. One parent strand is used as template for synthesis of new daughter strand (generating dsDNA plasmid) while other parent strand is forced out into a piece of ssDNA that can then be transferred to other strains through conjugation and then replicated into a dsDNA plasmid.

Describe the experiment and outcome from the Newcombe Discussion paper (Ok…4

sentences here):

Testing for adaptive vs. spontaneous mutation. Plated out E. coli, waited a few hours and then spread bacteria over plate to redistribute colonies. Sprayed with phage. If adaptive there wouldn’t be much of a difference in spread vs. unspread plates for phage resistant colones, 

however, since mutations occur spontaneously there were more colonies on the spread plates.

Incompatibility group:

Plasmids are placed into incompatibility groups based on their ability to co-­exist in a single cell with other plasmids. Incompatibility generally occurs because plasmids share replication or partitioning functions.