Term
| The main purpose of Microarray |
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Definition
to compare gene expression under 2 or more different envrironmental conditions.
- To get a snap shot of the expression of all genes.
- can see which genes are expressed or how much of expression.
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Term
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- 75 Base pairs
- ss DNA
- complementary to a certain gene
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- each spot is specific for a gene
- gene chip
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Term
| Transcriptional (mRNA) profiling experiment |
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Definition
- Harvest mRNA
- reverse transcribe to cDNA because it is more stable to use reverse transcriptase
- Label fluorescently cDNA (green, yellow, black, red)
- mix and hybridize to microarray
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Term
| The light intensity tell us what? |
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Definition
| It tells us how strong a gene is expressed in both samples |
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| lots of mRNA or lots of cDNA |
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