Term
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Definition
| utilizes visible light to detect small objects |
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Term
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Definition
aims light through condenser, through specimen, through objective lens, through magnifying lens to eye
4X, 10X, 40X, 100X multiplied by 10X objective |
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Term
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Definition
| uses a focused beam of electrons to "image" specimen and gain info on structure and composition |
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Term
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Definition
electron beam passed through thin tissue
encounter electron dense (block) and electron-lucent (pass) areas
magnification up to 120,000X |
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Term
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Definition
permits 3-D view of SURFACE of object
Reflected electrons are captured from metal coating on tissue, amplified, and image produced on screen |
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Term
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Definition
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Term
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Definition
| 100nm (10-7) to 1um (10-6) |
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Term
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Definition
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Term
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Definition
| smallest distance between two particles at which they can be seen as separate objects |
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Term
| Eye vs instrument resolution |
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Definition
eye = 0.2mm
light microscope = 0.2um
SEM = 2.5nm
TEM = 1.0nm |
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Term
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Definition
Acquisition
fixation
dehydration
infiltration
embedding (plastic resin or paraffin)
sectioning
staining
mounting |
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Term
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Definition
| crosslinks proteins and/or lipids to preserve original structure and chemical contents of cells |
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Term
| Embedding material for SEM |
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Definition
| plastic resin because paraffin cannot withstand thin sectioning or heat of beam |
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Term
| Size of sections for different scopes/embedding material |
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Definition
LM paraffin = 4-6um
LM plastic = 1-3um
TEM plastic = 50-70nm |
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Term
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Definition
most common staining combination
H = basic (cationic +) dye stains neg charge structures dark blue
E = acidic (anionic -) dye stains pos charge structures red or pink |
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Term
| basophilic cell structures |
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Definition
nucleic acids
glycosaminoglycans
acid glycoproteins
heterochromatin |
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Term
| acidophilic cell structures |
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Definition
| mitochondria, collagen, secretory granules |
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Term
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Definition
staining different color than expected
caused by polyanions causing clumping of dyes
toluidine blue stains cartilage purple |
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Term
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Definition
| structure stains same as dye |
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Term
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Definition
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Term
| masson trichome stain, alcian blue |
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Definition
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Term
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Definition
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Term
| periodic acid-schiff stain |
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Definition
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