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MICRO1
Stein
194
Biology
Undergraduate 2
10/10/2011

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Term
Leeuwenhoek
Definition
Bacteriology
Mycology
Protozoology
Parasitology
Phycology
Term
Linnaeus
Definition
Taxonomy
Term
Semmelweiss & Snow
Definition
Infection control
Epidemiology
Term
Pasteur
Definition
Industrial microbiology
Pasteurization - food and beverage tech
Term
Buchner
Definition
Microbial metabolism
Genetics
Genetic engineering
Term
Koch
Definition
Koch's postulates -> Etiology
Term
Ivanowski
Definition
Virology
Term
Beijerinck & Winogradsky
Definition
Environmental microbiology
Ecological microbiology
Term
Gram
Definition
Microbial morphology
Term
Lister & Nightingale
Definition
Antiseptic medical techniques
Hospital microbiology
Term
Jenner von Behring & Kitasato
Definition
Serology
Immunology
Term
Ehrlich
Definition
chemotherapy
Term
Fleming
Definition
Pharmaceutical microbiology
Term
Koch's Postulates
Definition
1. Suspected pathogenic organism should be present in all cases of the disease and absent from healthy animals
2. Suspected organism should be grown in pure culture
3. Cells from a pure culture of the suspected organism should cause disease in a healthy animal
4. the organism should be reisolated and show to be the same as the original
Term
Classification of bacteria
Definition
Morphology
Staining characteristics
rRNA sequences
Physiological properties
Environmental considerations
diseases that they cause
Term
Phylogenetic tree
Definition
isolate dna
PCR
squence analysis
generate phylogenetic tree
Term
Germ theory of disease
Definition
pathogens cause infectious diseases
Term
Allosteric regulation
Definition
regulating an enzyme by binding an effector molecule at allosteric site (not active site)
Term
amphibolic
Definition
a reversible metabolic reaction; a reaction that can be catabolic or anabolic
Term
anabolism
Definition
all of the synthesis reactions in a molecule
Term
anaerobic respiration
Definition
type of cellular respiration not requiring ocygen atoms as final electron acceptors
Term
anoxygenic
Definition
not producing oxygen
Term
antibiotic
Definition
antimicrobial agent produced naturally by an organism
Term
antibiotic antagonism
Definition
one antibiotic with least effect interferes with effects of another
Term
antibiotic synergy
Definition
combinations of drugs that improve efficacy of antimicrobials
Term
antimicrobial
Definition
any compound used to treat infectious disease; may also function as intermediate level disinfectant
Term
antiseptics
Definition
chemicals used to inhibit/kill microorganisms on skin or tissue
Term
archae
Definition
woese's taxonomy; domain which includes all prokaryotic cells having archael rRNA sequences
Term
aseptic technique
Definition
characteristic of an environment or procedure that is free of contamination by pathogens
Term
acidophile
Definition
microorganisms requiring acidic pH
Term
actin comet tails
Definition
actin structures that network off the cell to facilitate movement
Term
activation energy
Definition
the amount of energy needed to trigger a chemical reaction
Term
active site
Definition
functional site of an enzyme, the shape of which is complementary to the shape of the substrate
Term
active transport
Definition
the movement of a substrate against its electrochemical gradient via carrier proteins; requires cell energy in the form of ATP
Term
adhesions
Definition
attachment of microorganism to host cells
Term
aerobe
Definition
an organism that uses oxygen as a final electron acceptor
Term
aerobic respiration
Definition
type of cellular respiration requiring oxygen atoms as final electron acceptors
Term
aerotaxis
Definition
movement towards oxygen/air
Term
aerotolerant
Definition
can live in an environment with oxygen present
Term
agar
Definition
gel-like polysaccharide isolated from red algae and used as a thickening agent
Term
agar disk diffusion
Definition
measures the effect of an antimicrobial agent
Term
algae
Definition
eurkaryotic multi or unicellular photosynthetic organisms with simple reproductive structures
Term
alkalinophile
Definition
organism requiring high pH environments (alkaline)
Term
biodiversity
Definition
the number of species living in a given ecosystem
Term
biofilm
Definition
slimy community of microbes growing on a surface
Term
biogenesis
Definition
living things only come from other living things
Term
biological half-life
Definition
time it takes blood plasma concentration of something to half
Term
bioremediation
Definition
the use of microorganisms to remediate toxins in the environment to reclaim soils and waterways
Term
biosphere
Definition
the region of earth inhabited by living organism
Term
biotechnology
Definition
branch of microbiology in which microbes are manipulated to manufacture useful products
Term
broad spectrum drugs
Definition
antimicrobial that works against many different kinds of pathogens
Term
budding
Definition
in prokaryotes and yeasts, reproductive processes in which an outgrowth of the parent cell recieves a copy of the genetic material, enlarges and detaches
Term
capsule
Definition
glycocalyx composed of repeating units of organism chemicals firmly attached to the cell surface
Term
catabolism
Definition
all the decomposition reactions in an organism
Term
catalase
Definition
catalyzes decomposition of hydrogen peroxide
Term
cell envelope
Definition
cell membrane
Term
cell wall
Definition
in most cells, structural boundary composed of polysaccharide or protein chains that provide shape and support against osmotic pressure
Term
ATP
Definition
the primary, short term, recyclable energy molecule fueling cellular reactions
Term
autoclave
Definition
device that used steam heat under pressure to sterilize chemicals and objects that can tolerate moist heat
Term
autotroph
Definition
makes its own molecules of food
Term
bacillus
Definition
rod shaped prokaryotic cell
Term
bacteria
Definition
prokaryotic microorganism typically having cell walls composed of peptidoglycan. all cells with bacteria rRNA sequences
Term
bactericidal
Definition
kill bacteria
Term
bacteriocins
Definition
protein toxins produced by bacteria to inhibit growth of similar strains
Term
bacteriolytic
Definition
causing bacteria to lyse/die
Term
bacteriostatic
Definition
slow growth/reproduction
Term
batch culture
Definition
large scale closed system culture
Term
beta-lactamase
Definition
bacterial enzyme that breaks the beta-lactam ring of penecillin and similar molecules rendering them inactive
Term
binary fission
Definition
the most common method of asexual reproduction of prokaryotes, in which the parental cell disappears with formation of progeny
Term
binomial nomenclature
Definition
the classification method used in the linnaen way of taxonomy, which assigns each species both a genus name and a specific ephithet
Term
central metabolic pathways
Definition
the pathways used by bacteria to process major carbohydrates/other carbon sources for energy
Term
chemoheterotroph
Definition
uses chemicals/organic molecules for energy as its carbon source (storage of energy)
Term
chemotaxis
Definition
directed movement towards certain chemicals
Term
chemotherapeutic agent
Definition
a chemical that has the potential to destroy pathogenic microorganisms
Term
chemoorganoheterotroph
Definition
requires organic substrates to get carbon source
Term
chloroplast
Definition
light harvesting organelle found in photosynthetic eukaryotes
Term
chromosome
Definition
molecule of DNA associated with protein. in prokaryotes usually circular in area called nucleoid
Term
coccobacillus
Definition
a prokaryotic cell intermediate in shape between a sphere and a rod, elongated coccus
Term
coccus
Definition
spherical prokaryotic cell
Term
coenzyme
Definition
organic cofactor
Term
colony
Definition
visible population of microorganism living in one place, aggregation of cells avising from single parent cell
Term
competitive inhibition
Definition
when an inhibitory substance blocks enzyme activity by blocking active sites
Term
contact time
Definition
the amount of time water is allowed to contain the disinfectant to assure potability (retention time)
Term
cytoplasmic membrane
Definition
membrane surrounding all cells, and composed of a fluid mosaic of phospholipids and proteins
Term
cytoskeleton
Definition
internal network of fibers contributing to the basic shape of eukaryotic and rod shaped prokaryotic cells
Term
death phase
Definition
phase in a growth curve in which organisms are dying more quickly then they are being replaced
Term
detergent
Definition
positively charged organic sufactant
Term
diauxic growth
Definition
seuqential usage of two different sugars by certain bacteria
Term
diplococcus
Definition
pair of cocci
Term
disinfaction
Definition
the use of physical/chemical means to kill/inhibit microorganisms on inanimate objects
Term
DNA
Definition
deoxyribonucleic acid, made up of base pairs, makes up genetic material
Term
domain
Definition
any of the three basic types of cell groupings distinguished by Carl Woese, containing Linnaean taxon of kingdoms
Term
ecological niche
Definition
specific behaviors/heirachy and interaction of an organism with it's ecosystem
Term
ecosystem
Definition
all the organisms living in a particular habitat
Term
electron transport chain
Definition
series of redox reactions that pass electrons from one membrane bound carrier to another then to a final electron acceptor
Term
endospore
Definition
environmentally resistant structure produced by the transformation of a vegetative cell of genera bacillus or clostridium
Term
endotoxin
Definition
(lipid A), potentially fatal toxin released from the cell wall of dead/dying gram- bacteria
Term
endoflagella
Definition
special flagella of spirochetes taht spirals tightly around a cell, rather than protruding from it
Term
enzyme
Definition
an organic catalyst
Term
Escherichia Coli
Definition
gram- bacteria with pathogenic and nonpathogenic strains; nonpathogenic are symbiotic and live in the gut
Term
ethylene oxide
Definition
very toxic gas used as a disinfectant for heat sensitive materials
Term
etiology
Definition
study of causation of disease
Term
eukaryote
Definition
type of cell that has a nucleus
Term
exoenzyme
Definition
extracelluar enzyme, usually breaks down large molecules for entry into cell, eg.emylase
Term
facultative anaerobic
Definition
microorganism which can live with or without oxygen
Term
FAD
Definition
to FADH2, carries high energy electrons for oxidative phosphorylation
Term
feedback inhibition
Definition
(negative feedback) method of controlling the action of enzymes in which the end product in a series of reactions inhibits an enzyme in an earlier part of the pathway
Term
fermentation
Definition
in metabolism, the partial oxidation of sugar to release energy using an endogeneous molecule instead of an electron transport chain
Term
field of view
Definition
what is visible through microscope
Term
fimbriae
Definition
sticky, proteinaceous extensions of some bacterial cells that function to adhere cells to one another and to environmental surfaces
Term
fungi
Definition
eukaryotic organisms that have cell walls and obtain food from other organisms
Term
glycolysis
Definition
first step in catabolism of glucose via respiration/fermentation
Term
glycoprotein
Definition
integral membrane proteins, help with cell recognition, especially in immune system
Term
gram -
Definition
pink stained, thin layer of peptidoglycan outer layer
Term
gram +
Definition
purple stained thick layer of peptidoglycan, lipotechoic acid
Term
group translocation
Definition
active process by which substance being actively transported across cell membrane is chemically changed during transport
Term
growth factor
Definition
chemicals required in small amounts for metabolism (eg vitamins)
Term
generation time
Definition
time required for cell to grow and divide
Term
glycocalyx
Definition
sticky external sheath of prokaryote and eukaryotic cells
Term
halophile
Definition
organisms requiring >9% NaCl
salt loving
Term
hyperthermophiles
Definition
organisms that prefer extreme heat
Term
inclusion
Definition
deposited substance such as a lipid, gas vesicle stored within cytosol of cell
Term
Kreb's cycle
Definition
TCA cycle, 8 enzyme catalyzed cycles that transfer stored energy from acetyl CoA to coenzymes, NAD + FAD
Term
lag phase
Definition
phase in growth curve in which the organisms are adjusting to their environment
Term
lipid A
Definition
LPS component released from dead gram - cells, endotoxin
Term
Lipopolysaccharide (LPS)
Definition
molecule composed for Lipid A and polysaccharide found in gram - walls
Term
Lipotechoic Acid
Definition
LTA
Term
log phase
Definition
phase in growth curve in which population is most actively growing
Term
lophotrichous
Definition
having two or more flagella at one end
Term
lysozyme
Definition
antibacterial protein secreted in sweat/tears
Term
mesophile
Definition
organism that prefers room temperature
Term
microaerophile
Definition
microorganism that requires low levels of O2
Term
minimal inhibitory concentration (MIC)
Definition
smallest amount of drug that inhibits a pathogen
Term
NADH
Definition
coenzyme in redox reactins, carries electron from one cycle to another
Term
NAM/NAD
Definition
two glucose derivatives that make up pepridoglycan, OB bond @ C14 -> 5 c rings
Term
nitrogen cycle
Definition
biogeochemical cycle involving nitrogen fixation, ammonification, nitrification, denitrification and anammox reactions
Term
orepeat
Definition
Term
obligate aerobe
Definition
needs 02 to survive
Term
oxidative phosphorylation
Definition
the use of energy from redox reactions to attach inorganic phosphate to ADP
Term
pasteurization
Definition
heating foods/liquids to kill microorganism that cause spoilage
Term
pentose/phosphate pathway
Definition
enzymatic formation of phosphorylated pentose sugars from glucose 6-phosphate
Term
periplasmic spaace
Definition
in gram -, space between cell membrane and outer membrane with peptiglycan/periplasm
Term
permease
Definition
membrane transport proteins, passive transport
Term
phenolic
Definition
compound derived from phenol molecules that have been chemically modified to denature proteins/disrupt cell membranes
Term
photphosphorylation
Definition
use of energy from light to attach inorganic phosphate to ADP
Term
pilus
Definition
tubule involved in bacterial conjugation
Term
plasmolysis
Definition
plasma membrane pulls away from cell wall, lack of water (osmosis0
Term
pleomorphic
Definition
in cell morphology used to describe a variabily shaped prokaryotic cell
Term
primary metabolite
Definition
directly involved in growth/development
compound synthesized during the log phase of growth
Term
protease
Definition
digests amino acids outside cell wall
Term
proton motive force
Definition
storage of energy across a membrane, uses electron transport chain to create gradients
Term
protoplast
Definition
cell wall completely removed, gram pos cell lost peptidoglycan layer
Term
protozoa
Definition
single celled eukaryotes that lack a cell wall and are similar to animals
Term
psychrophile
Definition
survives in cold temps
-5 to 20
Term
putrefaction
Definition
decomposition of proteins (stage of decomposition)
Term
resolution
Definition
the ability to distinguish between objects that are close together
Term
narrow spectrum
Definition
antimicrobial that works against fe types of pathogens
Term
rRNA
Definition
ribosomal, form of RNA which makes up ribosomes
Term
sanitization
Definition
process of disinfecting surfaces and utensils used by public
Term
secondary metabolite
Definition
not directly involved in growth/development
Term
slime
Definition
loose, water soluble glycocalyx
Term
spheroplast
Definition
cell wall partially removed, gram - cell lost peptidoglycan layer
Term
spirillum
Definition
stiff, spiral shaped prokaryotic cell
Term
spirochete
Definition
helical, corkscrew shaped bacteria (gram -)
Term
stationary phase
Definition
phase in a growth curve in which new organisms are being produced at same rate as old dying
Term
staining
Definition
using dyes to color cells, simple = 1 dye, complex = multiple dyes
Term
sterilize
Definition
Term
sterilize
Definition
eradication of all organisms including endospores and viruses
Term
substrate level phosphorylation
Definition
transfer of phosphate to ADP
Term
taxonomy
Definition
science of naming and classifying organisms
Term
techoic acid
Definition
found in gram + bacteria
Term
thermophile
Definition
organism that lives in high temp environments
Term
vibrio
Definition
slightly curved rod shaped cell
Term
zone of inhibition
Definition
in a diffusion susceptibility test, a clear area surrounding drug-soaked disk where microbe doesn't grow
Term
saccharomyces cerevisiae
Definition
yeast, model euk organism
Term
streptococcus pneumoniae
Definition
cause of pneumonia, bacterial meningitis
Term
staphlococci
Definition
genus of coccus gram +, grow in bunches
Term
streptococcus
Definition
genus of coccus gram +, grow in chains
Term
twitching motility
Definition
1. pilus extends
2. pilus attaches
3. pilus retracts, pulling cell
Term
what do bacteria need to grow
Definition
carbon source
energy source
required elements
C,N,O,P,S,Na,K,Cl,Fe,Mg,Ca
trace elements
copper, manganese, selenium, zinc
Term
photoautotrophs
Definition
light - energy source
CO2 - carbon source
Term
photoheterotrophs
Definition
organic compounds - carbon source
Light - energy source
Term
chemoautotrophs
Definition
energy source- chemical compounds
carbon source - carbon dioxide
Term
chemoheterotrophs
Definition
organic compounds - carbon source
chemical compounds - energy source
Term
porins
Definition
found in gram negative bacteria,
exchange of substances between cytoplasm and periplasm by highly specific transport systems
change between periplasm and environment occurs via porins
Term
motility
Definition
random - no structure needed
Swimming - flagella
Twitching - type IV pili
Pushing - host-cel actin
Gliding - secreting polysaccharide
Term
means of growth measurement
Definition
direct cell counts
viable cell counts
measuring biomass
measuring cell products
Term
factors affecting growth
Definition
temp
oxygen requirement
pH
water availability
solute concentration
Term
superoxide dismutase
Definition
helps cells deal with oxygen - use oxygen as a terminal electron acceptor in ETC
Term
Presence of oxygen - harm bacteria how?
Definition
oxygen is highly reactive (oxidizing agent)
capable of degrading organic molecules (oxidizing)
Term
osmotic pressure affect on growth
Definition
affects shape of cell - shrink if hypertonic
expand if hypotonic
Term
Factors affecting enzyme function
Definition
presence/absense of cofactors and catlytic site
temp/pH -> denature
Term
bioaugmentation
Definition
introduction of group of microbes to treat contaminated soil or water
Term
bacteriocin
Definition
protein made by one bacterium which can kill a closely related bacterium
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