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Micro-quiz 2
Quiz 2- Lee Couch
53
Biology
Undergraduate 2
09/27/2010

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Term
Autotroph
Definition
Make C + E to make everything you need
Term
PhotoAutoTroph
Definition
C from organic Compound;
E from sunlight
Term
ChemoAutoTroph
Definition
C from CO2;
E from inorganic molecule
Term
Heterotrophs
Definition
get C and E from nutrients
Term
PhotoHeteroTrophs
Definition
C from organic compound;
E from sunlight
Term
ChemoHeteroTrophs
Definition
C from organic compound;
E from organic compound;
Term
What type of cell are our cells?
Definition
chemoheterotrophs
Term
What is the most medically important cell type?
Definition
chemoheterotrophic
Term
Harmful affects from O2
Definition
superoxide free radicals (O2-)
infear with proteins
specialize enzyme deals with this problem

Hydrogen Peroxide: (H2O2)
enzyme fix

Singlet O2: e- get boosted and fall back

Hydroxyl Radicals: OH-
disrupt protein and lipid
Term
Obligate Aerobes
Definition
STRICT!!!
must have O2 to survive
Term
Obligate Anaerobes
Definition
cannot have O2
ex. botchulism
Term
Facultative Anaerobes
Definition
Prefer O2 but can survive without it
Term
Microaerophiles
Definition
need a little bit of O2
too much O2 will kill them
Term
Aerotolerant
Definition
do not need O2 but can survive in the presence
Term
What is the average life span?
Definition
20-30min
Term
How does a cell grow?
Definition
grows to critical size
either will die or split through binary fission
Term
Binary Fission
Definition
dna replication
cell elongates
dna splits
septum forms
2 identical cells
Term
Growth Rate?
Definition
change in # of cells in a culture over a period of time
Term
What is on the axis of the growth rate?
Definition
# of cells on y
time on x
Term
Phases of growth rate?
Definition
lag
log
stationary
decline
Term
What does it lean if there is no lag?
Definition
taken from identical media
Term
what does it mean if there is a straight drop off of decline?
Definition
lysis, maybe too much water
Term
What happens if there is no stationary phase?
Definition
major environment change
Term
Affectors of Growth Cycle?
Definition
Temperature
pH
water
Chemicals
Term
What happens if a cell gets to cold?
Definition
it will either die, or metabolism will slow down
Term
What happens if the cell gets too hot?
Definition
Proteins will denature
Term
Psychrophiles
Definition
hate heat
optimum= 10c
glaciers, Antarctica
Term
Faculative Psychrophiles
Definition
optimum= 20c
refrigerator
Term
mesophiles
Definition
most common!!!
optimum 37c
Term
thermophiles
extreme thermophiles
Definition
40-75
extreme= >75
Term
whats our average pH?
Definition
7.4
Term
does media have to have the same pH? if so how do we accomplish this?
Definition
yes
buffers!
Term
three names of cells based on pH?
Definition
acidophiles <6
alkaphiles >8
Neutraphiles 6-8
Term
Halophiles?
Definition
prefer salty environments
>15
hypertonic solutions
Term
Halotolerant
Definition
with stand up to 15% salt!
gram +
Term
Xeriphiles
Definition
dry environments
Term
Chemicals affect what?
Definition
Carbon, Hydrogen, Oxygen, Nitrogen, Phosphorus, Sulfer, Trace Elements
Term
Chemically Defined (synthetic) Media
Definition
know exactly what its made of
Term
Complex (nonsythetic)
Definition
mixed with auger, dont know whats in it, most of what we use!
Term
Anearobic Media
Definition
becomed Reducing Media
obligate anearobes
chemicals in media which release H
Term
Selective Media does what?
Definition
tests for gram + or gram -; only allows one to grow
Term
Differential Media?
Definition
specific nutrient to see if is metabolized by the bacteria; if so a color change appears.
Term
What does the Maconkey plate show metabolizing of?
Definition
lactose
Term
What does the EMB plate test for?
Definition
lactose
Term
What does the MSA plate test for?
Definition
mannitol
Term
Sharing/Preserving Media
Definition
refrigerating- up to 2 weeks
deep freezing- months-year
freeze-drying- long periods of time
Term
Controlling Growth:
Definition
Disinfection
Antisepsis
Asepsis
Sterilization
Commercial Sterilization
Term
Disinfection
Definition
killing or inhibiting growth on nonliving things
Term
Antesepsis
Definition
disinfecting living things
Term
Asepsis
Definition
absence of contamination
Term
Sterilization
Definition
complete destruction of microorganisms
Term
Commercial Sterilization
Definition
getting rid of medically important bacteria
Term
4 ways to destroy bacteria
Definition
harm cell wall
denature proteins
damage DNA/RNA
damage ribosomes
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