Shared Flashcard Set

Details

Micro Lab 8
Diagnostic Mycology
18
Medical
Professional
04/19/2009

Additional Medical Flashcards

 


 

Cards

Term
Hair, skin, nails sample collection
Definition
storage at 4C or room temperature is acceptable
Term
sterile body fluids and urine samples
Definition
stored at 4C for up to 12 hours ***Blood and CSF must be processed immediately***
Term
vaginal secretions samples
Definition
direct observation, clinical diagnosis; yeast infections (yum)
Term
respiratory secretions samples
Definition
no rush, specimens may be refrigerated if necessary
Term
tissue specimens
Definition
aseptic procedures, immediate transport to lab
Term
KOH preparation
Definition
clears keratinized tissue so fungi can be visualized easier
Term
India Ink stain
Definition
highlights hyaline capsule of cryptococcus neoformans
Term
Giemsa and Wright's stains
Definition
detect Histoplasma capsulatum in bone marrow or blood
Term
Periodic Acid-Schiff (PAS) and Gomori's Methenamine Silver (GMS) stains
Definition
best for demonstrating fungi in TISSUE
Term
Gram stain
Definition
not a routine procedure, but can visualize Candida and Cryptococcus
Term
Fungi culturing rules
Definition
need media w/ Carbon (glu) and Nitrogen; growth at 30C; for most cultures must wait 4 weeks to be negative
Term
Culturing of oral thrush or vaginitis yeast infections
Definition
only need to wait 7 days
Term
culturing dimorphic fungi
Definition
must wait up to 8 weeks for neg result
Term
inhibitory mold agar (IMA)
Definition
enriched medium w/ gentamicin or chloramphenicol that supports growth of almost all fungi; inhibits bacteria
Term
Brain-heart infusion agar w/ 5% blood
Definition
supports the growth of dimorphic fungi
Term
Serum assays
Definition
cryptococcal and histoplasma antigen tests only
Term
septate hyphae
Definition
all members of aspergillus
Term
non-septate hyphae
Definition
rhizopus and mucor
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