Term
Obligate aerobe,
microaerophile,
facultative anaerobe,
aerotolerant anaerobe,
obligate anaerobe,
capnophile |
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Definition
| Name the 6 bacterial growth requirements for oxygen and carbon dioxide. (the categories) |
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Term
| Organisms that do not require oxygen for life and reproduction. |
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Definition
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Term
| Grow only in the absence of molecular oxygen. |
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Definition
| What is an obligate anaerobe? |
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Term
| One or more enzymes (ie superoxide dismutase and catalase) |
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Definition
| What substances do organisms that use oxygen have to protect themselves from superoxide anions? |
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Term
| Anaerobes outnumber aerobes at mucosal surfaces (linings of oral cavity, GI tract, GU tract) |
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Definition
| Where do anaerobes outnumber aerobes, except where? |
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Term
Propionibacterium acnes
Eubacterium spp.,
peptostreptoccus |
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Definition
| These organisms are found on the skin. |
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Term
Bacteroides vulgatus
Bacteroides thetaiotaomicron
Bacteroides distasonis
Bifidobacterium
Clostridium
Eubacterium
gram-positive cocci |
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Definition
| These organisms are found in the GI tract. |
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Term
| Spore-forming anaerobic bacilli (known as "clostridia) |
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Definition
| What type of bacteria are those in the genus Clostridium? |
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Term
Two types of food poisoning:
Type A: relatively mild, self-limited GI tract illness
Type C: more serious (but rarely seen) type of GI tract illness |
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Definition
| What disease does Clostridium perfringens cause? |
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Term
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Definition
| What disease does Clostridium botulimum cause? |
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Term
| Tetanus (caused by the neurotoxin tetanospasmin) |
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Definition
| What disease does Clostridium tetani cause? |
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Term
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Definition
| The organism that causes gas gangrene is...? (also called myonecrosis) |
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Term
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Definition
| Pseudomembraneous colitis is the key illness caused by this organism. (also antibiotic-associated diarrhea). |
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Term
actinomyces
bifiobacterium
eubacterium
mobiluncus
lactobacillus
propionibacterium |
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Definition
| Examples of non-spore-forming, anaerobic, gram-positive bacilli. |
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Term
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Definition
| Key symptom is "nuggets" found in pus, in a patient who is immunocompromised with sinus tract drainage. |
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Term
| Mobiluncus curtisii + Bacteroides spp., gram-positive cocci, Peptostreptococcus spp., Garnerella vaginalis, Mobiluncus spp. |
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Definition
| Bacterial vaginosis is a synergistic infectious process involving which bacteria? |
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Term
B. fragilis group
Porphyromonas spp
Prevotella spp
Fusobacterium spp. |
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Definition
| The major grouops of anaerobic gram-negative bacilli include...? |
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Term
1. Porphyromonas gingivalis
Porphyromonas endodontalis |
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Definition
| Patient goes to dentist with an absecess. Dentist cultures the tooth Which of the following bacteria can possibly cause the infection? |
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Term
| Must be transported/processed as fast as possible AND with minimum exposure to oxygen. |
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Definition
| What are 2 important factors that must be taken into consideration when transporting specimens for anaerobic culture? |
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Term
| Ones collected via needle/syringe. |
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Definition
| What specimen is considered a better specimen for anaerobic culture than a swab? |
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Term
| 1. Macroscopic exam of specimen; 2-Prep of gram-stained smears for microscopic exam; 3-innoculation of appropriate plated/tubed media; 4-anaerobic incubation of inoculated media |
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Definition
| What procedures shold be performed on clinical specimens to recover anaerobic bacteria? |
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Term
| Those that have never never been exposed to oxygen or have been exposed only briefly. |
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Definition
| What is the ideal anaerobic incubation system? |
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Term
| 5-7 days to allow growth of slow-growing anaerobes; up to 3 weeks whenever Actinomyces spp. are suspected |
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Definition
| How long are anaerobic cultures routinely held in the lab? |
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Term
| 1. Foul odor upon opening anaerobic jar/bag; 2-colonies on anaerobically incubated SBA plates but not on the CO2-incubated SBA or CHOC plates; 3-good growth (>1mm in diameter) of gray colonies on the BBE plate; 4-colonies on either KBLB agar or anaerobic SBA plates that fluoresce brick-red under UV light or are brown-black in ordinary light; 5-double zone of hemolysis on SBA incubated anaerobically |
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Definition
| What evidence indicates the presence of anaerobes in cultures? |
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Term
| 1. Describe colony morphology and whether rowth occurred on each of selective & non-selective anaerobic SBA plate media used; 2-describe the gram-stain reaction/cell morphology; 3-set up aerotolerance test; 4-inoculate pure culture/subculture plate and add appropriate disks |
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Definition
| How are suspected colonies of anaerobes processed? |
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Term
| Special potency disks can be used. They may provide presumptive ID of the suspected anerobic organism |
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Definition
| How is the true gram stain reaction of an anaerobe determined? |
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Term
| Clostridium, Actinomyces, Propionibacterium, Bifidobacterium, Lactobacillus |
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Definition
| Aerotolerant anaerobes include: |
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Term
| WSodium polyanethol sulfonate (SPS) disk |
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Definition
| What disk is used to presumptively ID Peptostreptococcus anaerobius? |
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Term
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Definition
| What organism, growing on anaerobic culture, might show a double zone of hemolysis on anaerobically incubated SBA plate; gram stain reveals BOXCAR shaped,gram+ bacillus. |
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Term
| surgical therapy, hyperbaric oxygen, antimicrobial therapy, anti-toxins |
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Definition
| What are approaches to treating anaerobic infections? |
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