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Micro exam
Lab exam 1
45
Biology
Undergraduate 1
12/02/2012

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Term
What order of gram stain?
Definition
Crystal violet, gram's iodine, ethyl alcohol, safranin
Term
What is a differential stain?
Definition
Postive staining that uses a single dye, all cells are same color.
Term
What is moderant?
Definition
A chemical that fixes dye in or on cells by forming an insoluble compound and retention of the dye
Term
what is the primary stain in gram stain?
Definition
the crystal violet
Term
what is the mordant stain in a gram stain?
Definition
gram's iodine
Term
What is the decolorizer in a gram stain?
Definition
the ethyl alcohol
Term
What is a gram + bacteria mean?
Definition
has a thick cell wall and no outer membrane
Term
What is a gram - cell mean?
Definition
it has an outer membrane, a cytoplasmic membrane, and a thin cell wall.
Term
What are properties of nutrient agar? (2)
Definition
1-general purpose
2-not selective or differential
Term
What type of medium is blood agar?
Definition
differential and has extra nutrients
Term
What are properties of MacConkey agar? (4)
Definition
1-cotains bile salts
2-selects for gram -
3-selective and differential
4-a pH indicator
Term
What properties of mannitol salts agar? (3)
Definition
1-differential medium
2-seperates skin from streptococci
3-selects for halotolerant
Term
What type of agar us used for a urine culture?
Definition
Nutrient and regular agar, sometimes MacConkeys
Term
What is a postive oxidase test?
Definition
test subject turns purple
Term
What is a negative oxidase test?
Definition
subject has no change or is yellow
Term
What is a postive catalase test look like?
Definition
bubbles form
Term
What is an ELISA?
Definition
Enzyme linked imunosorbent assay
Term
What are characteristics of mueller-hinton plate? (2)
Definition
1-rich medium that doesn't inhibit growth
2-differential plate
Term
What color are gram - bacteria after the ethly alcohol is added?
Definition
clear
Term
what color is gram - bacteria when safranin is added?
Definition
red
Term
What properties of pseudomonas? (5)
Definition
1-gram 1
2-rod shapped
3-strict aerobe
4-catalase +
5-oxidase +
Term
What are properties of Bacillus Subtilis? (5)
Definition
1-gram +
2-rod shaped
3-strict aerobe
4-catalase +
5-oxidase +
Term
What properties of clostridium butyricum? (5)
Definition
1-gram +
2-rod shaped
3-strict aerobe
4-Catalase -
5-oxidase -
Term
What properties of Escherichia coli? (5)
Definition
1-gram -
2-rod shaped
3-faculatative anaerobe
4-catalase +
5-oxidase -
Term
What are properties of Neisseria meningiditis? (5)
Definition
1-gram -
2-diplococci
3-faculatative anaerobe
4-catalase +
5-oxidase +
Term
What are properties of Proteus vulgaris? (5)
Definition
1-gram -
2-rod shaped
3-faculatative anaerobe
4-catalase +
5-oxidase -
Term
What properties of Serratia marcesens? (5)
Definition
1-gram -
2-rod shaped
3-faculatative anaerobe
4-catalase +
5-oxidase -
Term
What properties of Shingella dysenteriae? (5)
Definition
1-gram -
2-rod shaped
3-faculatative anaerobe
4-catalase +
5-oxidase -
Term
What are properties of Staphylococcus epidermidis? (5)
Definition
1-gram +
2-cocci
3-faculatative anaerobe
4-catalase +
5-oxidase -
Term
What are properties of Streptococcus pyogenes? (5)
Definition
1-gram +
2-cocci
3-faculatative anaerobe
4-Catalase -
5-oxidase -
Term
What properties of Vibrio cholera? (5)
Definition
1-gram -
2-curved rod
3-strict aerobe
4-catalase +
5-oxidase +
Term
What is beta hemolysis?
Definition
complete lysis of RBC, produces a golden halo
Term
What alpha hemolysis?
Definition
partial lysis of RBC, produces a greensih tint
Term
What is epidemiology?
Definition
The study of epidemics, who gets sick and why
Term
what is an epidemic?
Definition
More than the usual number of cases
Term
What is a pandemic?
Definition
entire, effects worldwide
Term
What was the bacteria that affected Milwaukee?
Definition
Cryptosparidium
Term
Where did the crytosparuduim come from in milwaukee?
Definition
Came from human waste that ran into the water supply
Term
What is qualitative bactiera data?
Definition
what the bacteria actually look like
Term
What is qyantitative bacteria data?
Definition
the numbers of bacteria
Term
What causes dysentery?
Definition
Entamoeba
Term
What die Guardia cause?
Definition
diarrhea
Term
What dies Plasmodium cause?
Definition
Malaria
Term
what does toxoplasma cause?
Definition
stillbirths, fetal anomalies, CNS disease in immune compromised individuals
Term
How does yeast repropduce?
Definition
by budding
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