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MIcro Exam II - Lectures 7-9
Exam II Flashcards - Lectures 7-9
207
Microbiology
Professional
04/20/2011

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Term
Review Chart on Gram+/-
Definition
Review
Term
Pathogenic Gram + (cocci)
Definition
Staph, Strep, Entero
Term
Pathogenic Gram + (spore formers)
Definition
Bacillus, Clostridium
Term
Pathogenic Gram + (rods)
Definition
Corynebacterium, listeria
Term
Spores
Definition
Keratin coat, DPA (hallmark of spores, allows it to cluster Ca++), no metabolism.
Term
Gram - (rods)
Definition
Neisseria (diplococcus), treponema and Borrelia (spirochetes)
Term
Gram - (Others)
Definition
Chladydia, Rickettsiae - obligate intracellular parasites.
Term
Acid Fast
Definition
Mycobacterium
Term
Mycoplasma
Definition
No cell wall
Term
Function of Ca++ in spores
Definition
Stabilize DNA, protect from oxidative dmg.
Term
Bacillus shape, oxygen
Definition
Fac. aerobe, streptobacillus
Term
B. anthracis virulence factors
Definition
Capsule (makes it slippery), spores, toxins
Term
B. anthraces Toxins
Definition
Protective Antigen (PA), Edema Factor - which is PA+EF, allows fluid to accumulate, Lethal Factor (LF) Stimulates macs to release cytokines, which lyses the macs.
Term
How B. Anthracis causes damage
Definition
Cutaneous, Inhalation, GI
Term
Cutaneous Anthrax
Definition
Majority of cases, Red w/in 12-36 hrs, 20% mortality, progresses to sepsis.
Term
Inhalation Anthrax
Definition
Alveolar macs ingest spores, 95% fatal. Can progress to hemorraghic meningitis.
Term
GI Anthrax
Definition
Mortality near 100%
Term
Anthrax diagnosis
Definition
DFA (direct Fluor. Antibody), Lysis w/ gamma phage (virus that only infects it), PCR
Term
Treatment of B. anthraces
Definition
Ciproloxacin for cutaneous, could use penicillin and doxycycline but penicillin resistance has been reported, Cipro or doxycycline for inhalation
Term
B. cereus causes
Definition
food poisoning, eye infections, spore former.
Term
3 forms of B. cereus
Definition
Emetic form - heat stable enterotoxin, Diarrheal form - infection not intoxication, Ocular form - less common - eye injury
Term
Autoclave indicator
Definition
B. stearothermophilus
Term
Listera shape, oxygen preferences
Definition
Fac. aneaerobe, rod.
Term
How to clear Listeria
Definition
Need CMI, abs not effective.
Term
Listeria is a facultative intracullular ____
Definition
Parasite
Term
What forms actin rockets
Definition
Listeria - won't be exposed to I/S. In lunch meat, dairy.
Term
Diseases caused by Listeria
Definition
Food poisoning, at risk are elderly, prigs, newborns, CMI defects.
Term
Treatment of Listeria
Definition
penicillin OR gentamicin + penicillin or ampicillin
Term
Where to find Listeria
Definition
Lunch meat, dairy, soft cheeses, raw veggies.
Term
Corynebacterium is opportunistic (T/F)
Definition
True, few are true pathogens.
Term
What causes Diphtheria
Definition
Corynebacterium
Term
Symptoms of Diptheria
Definition
Pseudo membrane in throat, bull neck, cardiac complication from A-B exotoxin.
Term
What type of toxin is diphtheria toxin?
Definition
A-B toxin - 2 parts
Term
A-B toxin, explain
Definition
A-Action - A subunit goes in and stops translation, B is for binding.
Term
What 2 cell types do B toxins bind?
Definition
Heart, nerve
Term
Treatment of Diptheria
Definition
Antitoxin, Ab - penicillin or erythromycin to remove bacteria.
Term
What kind of vaccine is for diphtheria?
Definition
Toxoid
Term
Size/shape/Oxygen Pref. of mycobacterium
Definition
Aerorbic acid fast rods
Term
Damage of mycobacterium is from what
Definition
Your I/S.
Term
Clinical test for Mycobacterium?
Definition
PPD, Acid Fast stain.
Term
Primary infection of Mycobacterium
Definition
Taken up by macs - aerosolized transmission. Ghon's complex forms - inflammation, WBCs, tissue destruction.
Term
Secondary Infection of Mycobacterium
Definition
Reactivation of infection, Pulmonary TB or miliary TB, Granulomas - inflammation, chronic fever, night sweats, weight loss, cough.
Term
Treatment of TB
Definition
RIP, Rifamycins (inhibit transcription) Isoniazid (cell wall synthesis inhibitor), Pyrazinamide (unclear mode)
Term
Prevention of TB
Definition
BCG vaccine.
Term
What causes leprosy
Definition
Mycobacterium leprae
Term
What type of immunity is needed to clear M. leprae?
Definition
CMI - it's an intracellular bacteria.
Term
Forms of Leprosy?
Definition
Lepromatous leprosy - highly infectious, skin destruction, takes >1 yr. of treatment. Tuberculoid leprosy - Nerve damage, low infectivity, hypopigmented skin lesions.
Term
Which form of leprosy is the most serious?
Definition
Lepromatous leprosy.
Term
Epidemiology of Mycobacterium avium?
Definition
Pulmonary disease or disseminated disease, especially in immunocompromised, immunosuppressed people.
Term
Treatment of M. avium
Definition
Prophylaxis in HIV patients - azithromycin, treat infections w/ refampin or isoniazid.
Term
Shape/oxygen prefs. of Clostridium?
Definition
Gram + rods, mostly anaerobic
Term
Where does clostridium live?
Definition
Soil, sewage, GI of humans and animals.
Term
C. Perfringens infection site…
Definition
Wound infection/gas gangrene or gastroenteritis.
Term
C. perfringens infection site that is most severe
Definition
Wound infection. Tissue destroyed – blood supply cut off – necrosis. Myonecrosis – spreads deeper and kills muscle. Diabetics get this in ischemic limbs (lack of blood flow and therefore oxygen). Treat by keeping wounds CLEAN and penicillin. High mortality for would infections because more than 12 toxins
Term
Lecithinase is associate w/ which bacteria
Definition
C. perfringens - hydrolyzes host cell membranes and mediates massive cell destruction, tissue destruction, hepatic toxicity, and myocardial dysfunction.
Term
What disease does Clostridium tetani cause, and where?
Definition
Tetanus, wound infections - fatal in newborns when umbilical stump gets infected.
Term
Where is C. tetani found?
Definition
Soil and GI tracts of humans and animals.
Term
Two important toxins of C. tetani?
Definition
Tetanolysin (hemolysin) and Tetanospasmin (A-B toxin, a neurotoxin).
Term
Treatment of C. tetani
Definition
Wound debridement, Metronizadol, Antitoxin.
Term
Clostridium botulinum causes what?
Definition
Food poisoning, wound botulism, and infant botulism.
Term
Why do you die with botulism?
Definition
You can't work your lung muscles.
Term
Treatment of botulism?
Definition
Removal from GI tract if it's there - metronizadole or penicillin, Can also use an antitoxin.
Term
Prevention of C. botulinum?
Definition
Destroy spores in food, prevent germination, destroy the toxin.
Term
Clostridium dificile is a component of normal flora (T/F)
Definition
True for 5% of the population.
Term
What causes Pseudomembranous colitis?
Definition
C. dificile, due to clindamycin wiping out normal flora.
Term
Treatment of C. dificile?
Definition
Stop antibiotic you were taking and switch to a safe one.
Term
Lactobacillus shape, oxy preferences, etc…
Definition
Gram + rod, anaerobe, found in the mouth.
Term
Is Lactobacillus acidogenic or acidouric?
Definition
Both, easy one.
Term
Actinomyces gram status, shape, oxy prefs
Definition
Gram + Fac. anaerobe or strict anaerobe.
Term
Who has filamentous hyphae
Definition
Actinomyces
Term
Does Actinomyces have a role in caries?
Definition
Yes. A. israelii is part of normal flora but can be opportunistic, can find in plaque, gingivitis, granulomatous lesions. Treat w/ penicillin. Too much for one flashcard?
Term
Most Gram- are rods except...
Definition
Neisseria (diploccus) and Treponema and Borrelia (spirochetes)
Term
Gram- have what in their cell wall
Definition
Lipopolysaccharide.
Term
What is the mechanism of LPS?
Definition
Endotoxin, released when bacteria lyse, active at the site of infection, reacts w/ macs and causes shock in large quantities.
Term
Which is unstable, toxic in tiny amounts, small, and specific to a cell (exotoxin or endotoxin)
Definition
Exotoxin.
Term
Which is needed in higher doses, stable, can't be converted to a toxoid, causes a fever (endotoxin or exotoxin)?
Definition
Endotoxin
Term
Shape, arrangement, gram status of Neisseria?
Definition
Gram-, aerobic diplococci.
Term
What diseases does Neisseria cause?
Definition
N. gonorrhea (STD), and N. meningitides (meningitis).
Term
What diseases cause meningitis in < 6 mos.?
Definition
Listeria, E. coli, S. agalactiae.
Term
What diseases cause meningitis in > 6 mos. people?
Definition
S. pneumoniae, M. mengitides, H. influenzae.
Term
Who is most at risk of N. meningitides?
Definition
Children and young adults. Mortality near 100% if not treated.
Term
What is the mortality of meningitis?
Definition
Near 100%.
Term
What is the disease process of N. meningitides?
Definition
Exposure through close contact with secretions, May lead to colonization, Bacteria engulfed by epithelial cells of the mucosa, Penetrate into blood vessels then cross blood brain barrier, Grow in CSF, Symptoms: fever, sore throat, headache, fever, stiff neck, convulsions, Bacteria shed endotoxin into circulation and macs are stimulated, Cytokines lead to vascular hemorrhage and coagulation, IF they recover 10%-20% of patients will have lasting cognitive impairment
Term
What is the diagnosis/treatment of N. meningitides?
Definition
Spinal tap, treat w/ penicillin, also rifampin, ciprofloxacin, tetracycline.
Term
Is there a N. meningitides vaccine?
Definition
Yes, mostly for high risk people - military, students, kids <2.
Term
What are the virulence factors of N. meningitides?
Definition
Capsule, Endotoxin - LPS, Pili, Adhesins, IgA protease, Transferrin binding protein (iron for the bug), LOS - lipooligosaccaride - which inflames the innate I/S.
Term
What is the 2nd most prevalent STD in the US?
Definition
Gonorrhea (1st is Chlamydia). Incidence exceeds reported cases b/c of asymptomatic carriers.
Term
What are the symptoms of gonorrhea?
Definition
Pelvic inflammatory disease, septic arthritis, conjuctivitis and blindness in newborns.
Term
What are the stages of infection of N. gonnorrhea?
Definition
Direct contact – only short term survival on fomites, Attach to epithelial surface with fimbriae, pili and Opa protein, Invade underlying connective tissue, 2-6 days you get inflammatory reaction that may or may not lead to symptoms, asymptomatic in 10% of men and 50% of women, asymptomatic carriers are the reservoir, Stimulate complement, but do not produce lasting immunity
Term
What is the diagnosis of N. gonorrhea?
Definition
Diagnosis of Gram- diplococci (possibly IN neutrophils) from exudates.
Term
What is the treatment of gonorrhea?
Definition
Cephalosporins, and not penicillin.
Term
What are the virulence factors of N. gonorrhea?
Definition
NO CAPSULE, fimbriae, pili, and Opa protein. IgA, B-lactamase, lactoferrin-binding proteins, Por protein, LPS and LOS
Term
Does N. gonorrhea have a capsule?
Definition
NO
Term
Which bacteria exhibits the Por Protein?
Definition
N. gonorrhea.
Term
Describe the gram status, oxy. prefs and shape of Enteobacteriaceae family?
Definition
Gram- rods, aerobic, or fac. anaeorbes.
Term
What is the common site of infection of enterobacteriaceae?
Definition
GI tract. Can also be in CNS, lower resp. tract, blood stream, UTI
Term
What family contains lactose fermenters?
Definition
Enterobacteriacae.
Term
What is the shape/oxy prefs, gram status of E. coli?
Definition
Fac. anaerobic rod, gram-.
Term
What is ETEC/EHEC?
Definition
E.coli, ETEC – enterotoxigenic E. coli Not person-person spread. Very large amt. needed for infection. EHEC – enterohemorrhagic E. coli – E. coli 0157:H7, for example. Small amt. needed for infection - ~100 bacteria. Low infection dose = easy to cross-contaminate.
Term
What bacteria is most associated with UTI?
Definition
E. coli.
Term
What is the most common cause of Gram negative sepsis?
Definition
E. coli.
Term
What are the virulence factors of E. coli?
Definition
Pili, enterotoxins, endotoxins, shiga toxins.
Term
What is the shape/gram status, oxy prefs of salmonella?
Definition
Gram-, fac. anaerobes, rod.
Term
Where is salmonella found?
Definition
Animals and animal products. Transmitted oral/fecal, contaminated food/water.
Term
What causes Typhoid Fever?
Definition
Salmonella typhi. Most serious in the genus, severe diarrhea.
Term
What is the treatment for Salmonella typhi?
Definition
Chloramphenicol - binds ribosome and stops protein synthesis.
Term
Which is more serious - Salmonella typhi or Salmonella typhimurium?
Definition
Typhi. Typhimurium - found in poultry/eggs.
Term
What are the toxins of salmonella?
Definition
Enterotoxins, Fimbriae, cytotoxin, survive intracellularly.
Term
What is the shape/oxy prefs, gram status of shigella?
Definition
Gram-, fac. anaerobic rods.
Term
How is shigella spread?
Definition
Fecal/oral.
Term
What is the most severe form of shigella?
Definition
Shigella dysenteriae.
Term
Which is more invasive - Salmonella or Shigella?
Definition
Salmonella - Shigella has Endotoxin, Shiga toxins,
Term
What is the shape/oxy prefs gram status of Yersinia?
Definition
Fac anaerobic rods, Gram-, causes zoonotic disease.
Term
What causes the bubonic plague?
Definition
Y. pestis. CDC cat. A. bioweapon. 75% mortality if not treated.
Term
What are the reservoir/vector of bubonic plague?
Definition
Rodents are the reservoir, fleas are the vector.
Term
What causes the pneumonic plague?
Definition
Y. pestis, aerosol variant of bubonic plague. 90% mortality.
Term
What is the reservoir of Y. enterocolitica?
Definition
Farm animals.
Term
Rank Y. pestis, Y. enterocolitica, and Y. pseudotuberculosis?
Definition
Pestis/Entero/Pseudo.
Term
What does Klebsiella pneumoniae cause?
Definition
UTI, wound and soft tissue infections, pneumonia. Gram- rods.
Term
What has the enzyme urease?
Definition
Proteus mirabilis. Causes UTIs, gram- rods. Urease splits CO2 and ammonia, causing pH balance problems in pee.
Term
Describe shape, gram status and oxy prefs. of Vibrio cholerae?
Definition
Gram-, Fac. anaerobic curved rods.
Term
What disease does Vibrio cholerae cause?
Definition
Cholera, aka butt urine. 20L/day. Results in hypovolemic shock.
Term
How is V. cholerae transmitted?
Definition
Contaminated shellfish, water.
Term
What is the treatment for cholera?
Definition
Oral vaccine - multiple doses per year. Hygiene is KEY. Treat w/ fluids and electrolytes.
Term
Place the virulence of the following in order...Y. pestis, Vibrio and Salmonella, Strep Pneumoniae, Shigella.
Definition
Vib/Sal, Shigella, Strep Pneum., Y. pestis.
Term
Describe the shape, and oxy prefs of Campylobacter...
Definition
Vibrio (spiral), microaerophiles.
Term
Name the 2 Camylobacter species that cause gastroenteritis in the US...
Definition
C. jejuni - major foodborne illness in developed countries, zoonotic (sheep/chickens), water, milk, meat. Rehydration is sufficient. C.coli - associated w/ pork.
Term
What is the association between Campylobacter and Guillain-Barre (pronounced Gil-ayn Bare) Syndrome?
Definition
Antigenic crossreactivity btw LPS and peripheral nerve gangliosides. Causes peripheral nerve dmg, auto-immune disease.
Term
Describe gram status and shape of Helicobacter?
Definition
Gram-, pleomorphic rods.
Term
What is the transmission of Helicobacter?
Definition
Fecal-oral, humans are the reservoir (40-50% of adult stomachs have it) flies can be vectors. Zoonotic from cats, dogs, other mammals.
Term
How does helicobacter survive the conditions in the stomach?
Definition
Urease - causes peptic ulcers. Can cause esophageal carcinomas.
Term
How is helicobacter treated?
Definition
Antibiotics clarithromycin and proton pump inhibitors.
Term
What is the gram status, shape and oxy prefs of Pseudomonas?
Definition
Gram-, obligate aerobe rods.
Term
Where is pseudomonas normally found?
Definition
Soil, water, hospitals - drinking fountains, sinks, soap, dishes.
Term
Pseudomonas doesn't form biofilms - T/F?
Definition
False - it does form biofilms.
Term
What produces a greenish pus, sweet smelling infection?
Definition
Pseudomonas.
Term
Is pseudomonas part of the normal flora?
Definition
No.
Term
Typical pseudomonas infections?
Definition
UTIs, swimmers ear, wound infections in burn patients, pneumonia, sepsis, osteomyelitis.
Term
What are the Virulence factors for Pseudomonas?
Definition
Capsule, pili, LPS, exotoxins, elastase, hemolysins, ABX resistance.
Term
T/F - Monotherapy is effective in treating Pseudomonas.
Definition
False - almost always ineffective.
Term
What is the shape/gram status/oxy prefs of Haemophilus?
Definition
Pleomorphic Gram- rods, often form long filaments.
Term
T/F Haemophilus infleunzae causes the flu.
Definition
False - meningitis.
Term
T/F Haemophilus influenzae is the most common cause of meningitis 3 mos-3 yrs int he US.
Definition
False - most common in the world, but we vaccinate.
Term
Other diseases caused by Haemophilus influenzae?
Definition
Pharyngitis, pneumonia in adults, conjuctivitis, sinusitis, cellulitis.
Term
What is unique about the HiB vaccine?
Definition
Conjugate polysaccharide (capsule) + protein (helps improve response to the vaccine in kids).
Term
What is the gram status/shape of Bordetella pertussis?
Definition
Gram- coccus, strict aerobe.
Term
What causes whooping cough?
Definition
Bordetella pertussis.
Term
How is B. pertussis transmitted?
Definition
Aerosol - highly contagious. Increasing in the US in older kids and adults.
Term
T/F pertussis toxin is an A-B toxin.
Definition
True.
Term
What is the mechanism of damage of whooping cough?
Definition
Bacterium attach to epithelial cells by fimbria.
Term
How is B. pertussis infections treated/prevented?
Definition
Toxoid vaccine (pertussis toxin) and macrolides (like erythromycin) - protein synthesis inhibitors.
Term
T/F Legionella pneumophilia is person-to-person spread.
Definition
False - common src (like cooling towers, A/C). Sporadic worldwide occurrence from aerosols.
Term
What type of immunity do you need to recover from Legionella pneumophilia?
Definition
CMI - it's intracellular. 75% mortality if immunocomrpomised, 15% if healthy.
Term
What diseases does Legionella pneumophila cause?
Definition
Legionnaire's disease (pneumonia) and Pontiac fever (flu-like, no pneumonia).
Term
T/F Legionella pneumonia is a facultative intracellular parasite.
Definition
True - need CMI to clear.
Term
Describe shape/oxy prefs/gram status of Legionella pneumophila.
Definition
Gram- rods, fastidious obligate aerobes.
Term
How is Legionella pneumophila treated?
Definition
Macrolides and fluroquinolones.
Term
What is gram status/oxy prefs/shape of Bacteroides?
Definition
Gram- pleomorphic rod shaped anaerobes.
Term
T/F Bacteroides is normal flora of the GI tract and oral cavity.
Definition
True - some can cause disease in oral cavity.
Term
T/F Bacteroides produces colleganes.
Definition
True - important in progression of periodontal disease.
Term
What causes trench mouth?
Definition
Bacteroides.
Term
How is Bacteroides treated?
Definition
Clindamycin.
Term
B. fragilis causes anaerobic infection in the peritoneal cavity - T/F.
Definition
True. Causes abcesses.
Term
What is the most common source of infection in the blood?
Definition
Bacteroides.
Term
What is the gram status/oxy prefs/shape of Porphyromonas gingivalis?
Definition
Gram- anaerobe rod.
Term
What is the role of Porphyromonas gingivalis in periodontal disease?
Definition
Degrades collagen in perio disease.
Term
Which has higher virulence - Porphyromonas gingivalis or Prevotella inermedia?
Definition
P. gingivalis.
Term
What is black when grown on blood agar?
Definition
Prevotella intermedia and Porphyromonas gingivalis.
Term
Name the genus that contains oral spirochetes.
Definition
Treponema - suspected to be main causer of perio disease.
Term
Describe the shape/gram status/oxy prefs of Treponema.
Definition
Gram- anaerobes, spirochetes.
Term
What causes Syphilis?
Definition
Treponema pallidum. #3 STD in the US.
Term
How is T. pallidum visualized?
Definition
Dark field microscopy. DFA test also used to diagnose.
Term
How is syphilis treated?
Definition
Pen G(injected).
Term
What is the 1st stage of syphilis?
Definition
Canker at site of infection.
Term
What is the 2nd stage of syphilis?
Definition
Bacteremia w/ rash.
Term
What is the 3rd stage of syphilis?
Definition
Organ damage, neurological dmg - granulomas called GUMMAS.
Term
What is congenital syphilis - and dental ramifications?
Definition
From mom to baby - crosses placenta. Hutchinson's incisors and Mulberry Molars.
Term
What is the shape/oxy prefs/arrangement of Borellia burgdorferi?
Definition
Gram- spirochetes.
Term
What causes Lyme disease?
Definition
Borellia burgdorferi.
Term
Where does Lyme disease occur mostly?
Definition
Endemic in US, NE and mid-atlantic states.
Term
Fleas are the vector for Lyme disease - T/F.
Definition
False - ticks.
Term
What are the symptoms of Lyme disease?
Definition
Rash at bite, neuro symptoms if not treated, cardiac dysfunction, arthritis.
Term
How is Lyme disease treated - what about late stage?
Definition
amoxicillin, doxycycline, tetracycline. Late stage is penicillin.
Term
What is the gram status of Mycoplasmas?
Definition
Trick question - no cell wall so no gram status.
Term
What does Mycoplasma cause?
Definition
Pneumonia.
Term
How is Mycoplasmas treated?
Definition
Erythromycin, tetracycline, fluoroquinolone.
Term
Describe the gram status and shape of Rickettsia.
Definition
Gram- cocci, obligate intracellular parasites.
Term
T/F Mycoplasma can't make ATP on its own.
Definition
False - Rickettsia can't.
Term
What is the vector for Rickettsia?
Definition
Arthropods (I think later it says no vector - air).
Term
What causes typhus, Rocky Mtn. Spotted Fever, Q fever?
Definition
Rickettsia.
Term
What causes typhus?
Definition
Rickettsia prowazekii.
Term
What is the vector for Rickettsia prowazekii?
Definition
Louse.
Term
What are the symptoms of typhus?
Definition
Rash everywhere BUT face. Fever, headache, fatigue.
Term
How is typhus treated?
Definition
Tetracycline.
Term
What causes Rocky Mtn. Spotted Fever?
Definition
Rickettsia rickettsii.
Term
Where is Rocky Mtn. Spotted Fever found?
Definition
SW US and Eastern seaboard.
Term
How is Rocky Mtn. Spotted Fever spread?
Definition
Humans via ticks.
Term
Where does Rocky Mtn. Spotted Fever grow in the cell?
Definition
The nucleus.
Term
How is Rocky Mtn. Spotted Fever treated?
Definition
Tetracycline (same for all Rickettsia).
Term
T/F Chlamydia is an obligate intracellular parasite?
Definition
True.
Term
What is the leading STD in the US?
Definition
Chlamydia - may have co-infection w/ gonorrhea.
Term
What is infected in Chlamydia?
Definition
Urethra/Fallopian Tubes, also eye infections (but rare in US, treatable).
Term
What does Chlamydia trachomatis cause?
Definition
Chlamydia.
Term
What does Chlamydia pneumoniae cause?
Definition
Pneumonia.
Term
Which bacteria have A-B toxins?
Definition
B. pertussis, Clostridium, Vibrio, Corynebacterium.
Term
Which bacteria has 12 toxins?
Definition
C. perfringens
Term
Large drift, small drift (Influenza A/B/C).
Definition
A/B
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