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Micro exam 2
definition
59
Microbiology
Undergraduate 3
02/26/2012

Additional Microbiology Flashcards

 


 

Cards

Term
Virion
Definition
individual mature virus material
Term
spikes
Definition
exposed proteins on the outside of an envelope virus
Term
bacteriophage
Definition
a virus that specifically infects bacteria
Term
Restriction Endonuclease
Definition
an enzyme present normally in cells that cleaves specific locations on DNA; used to splice genes in genetic engineering
Term
Host Range
Definition
Limitation imposed by the characteristics of the host cell on the type of virus that can successfully invade it
Term
Chronic latent state
Definition
can last weeks or host's lifetime; several can periodically reactivate
Term
Oncoviruses
Definition
mammalian viruses capable of initiating tumors.
Term
Prions
Definition
  • misfolded  proteins that contain no nucleic acid
  • resistant to visual sterilization techniques
  • can cause transmissible spongiform encephalopathies
Term
Satellite Virus
Definition

dependent on the other viruses for replication

ex: adeno-associated virus, delta agent

Term
nutrition
Definition
process by which chemical substances (nutrients) are acquired from the environment and used in cellular activities
Term
essential nutrients
Definition
must be provided to an organisim 
Term
macronutrients
Definition
  • required in large quantities
  • plays roles in cell structure and metabolism
Term
micronutrients
Definition
  • required in small amounts
  • involved in enzyme function and maintenance of protein structure
Term
heterotroph
Definition
must obtain carbon in organic form made by the living organisms 
Term
autotroph
Definition
  • an organism that uses CO2 as its carbon source
  • not nutrionally dependent on other living things
Term
chemotroph
Definition
gain energy from chemical compunds
Term
phototrophs
Definition
gain energy from photosynthesis
Term
osmosis
Definition
diffussion of water
Term
aerobe
Definition
uses O2 & can detoxify it
Term
facultative anaerobe
Definition
able use or not use O2
Term
microaerophillic
Definition
requires only a small amount of O2
Term
anarobe
Definition
does not use O2
Term
obligate anarobe
Definition
can't use or detoxify O2
Term
aerotolerant
Definition
does not use O2 but it is able to grown in an O2 environment
Term
capnophile
Definition
grows best in an CO2 environment
Term
alkalineophile
Definition
grows at extreme alkaline pH
Term
halophile
Definition
grows at high concentration of salt 
Term
barophile
Definition
can survive under extreme pressure but will rupture if exposed to normal atmospheric pressure
Term
mutualism
Definition
both members benefit
Term
commensalism
Definition
1 member benefits, the other isn't harmed
Term
parasitism
Definition
1 benefits while the other is harmed
Term
synergism
Definition
members cooperate & share nutrients
Term
antagonism
Definition
some members are inhibited or destroyed by others
Term
turbidometry
Definition
measure of cloudiness, reflects bacterial population size
Term
genotype
Definition
all types of genes canstitute the genetic makeup
Term
phenotype
Definition
expression of the genotype creates observable traits (physical)
Term
transcription
Definition
info shared on the DNA molecule is conveyed to RNA molecules
Term
translation
Definition
process in which the infor contained in RNA molecule is used to produce proteins
Term
polyribsomal complex
Definition
allows for the synthesis of many protein molecules simultaneously from the same mRNA molecule
Term
mutation
Definition
a change in phenotype due to change in genotype
Term
wild type
Definition
a natural, nonmutated characteristic (wild strain)
Term
binary fission
Definition
division of bacterial cells (transverse)
Term
sterilization
Definition
process to destroy all viable microbes; including viruses & endospores
Term
disinfection
Definition
process to destroy vegetative pathogens NOT endospores on inanimate objects
Term
antiseptic
Definition
disinfectant applied directly to exposed body surfaces
Term
sanitization
Definition
any cleansing technique that mechanically removes microbes
Term
thermal death time
Definition
shortest length of time required to kill all test microbes at a specfied temperature
Term
thermal death point
Definition
lowest temperature required to kill all microbes in a sample in 10mins
Term
tyndallization
Definition

internittent sterilization for substances that cannot withstand autoclaving 

  • repeated steaming and incubation cycles needed
  • disinfectant

 

 

 

Term
pasteurization
Definition

heat is applied to kill potential agents of infection and spoilage w/o destroying the food flavor or value

  • not sterilization
  • lowers microbe count
Term
desiccation
Definition

gradual removal of H2O from cells, leads to metabolic inhibition

  • cells still retain ability to grow when H2O is reintroduced
Term
lyophilization
Definition
freese drying; preservation
Term
filtration
Definition

physical removal of microbes by passing a gas or liquid through filter

  • used to sterilize heat sensitive liquidds & air in hospital isolation units & industrial clean rooms
Term
halogens
Definition

group of chemicals w/anti microbial applications 

  • chlorine: denatures proteins by disrupting disulfide bonds
  • iodine (betadine): interferes w/disulfide bonds of proteins
Term
phenolics
Definition

disrupts cell walls & membranes & precipitate proteins

 

Term
alcohols
Definition

ethyl, isopropyl in solutions of 50-95%

  • acts as surfactants, dissolving membrane lipids & coagulating proteins of vegetative bacterial cells & fungi
Term
hydrogen peroxide
Definition
provides highly reactive hydroxyl-free radicals that damage protein & DNA while also decomposing to O2 gas (toxic to anarobes)
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