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| The ability of microbes to utilize N2, as a nitrogen source is called.... |
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| bacterial growth refers to an increase in the ____ of bacterial cells |
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| an unknown organism grows at the top of a tube of thioglycolate broth.. This organism is _____ for oxygen requirements |
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| salt agar tends to inhibit the growth of most organisms except staph. this is an example of _____ media |
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| this method , cells are added to melted agar, and poured into a dish |
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| bacteria reequire nitrogen for the synthesis of |
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| what do all these bacteria have in common? Bacteria in the rumen of cattle, sheep bacteria in a sewage treatment plant, bacteria growing in the middle ear of chronic otitis, bacteria on the teeth |
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| bacteria are most likely growing in biofilms... |
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| an organism that grows both in the presence and the absence of o2 and uses oxygen when it is avail |
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| culture medium consisting of agar, peptone, and beef heart extract.. |
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| niacin, when added to a medium would be considered |
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| martian soil is inoculated into a glucose-containing med. the radioactive form of c, is used in the glucose. after incubation for 5 days, what provides evidence suggesting there is life on mars |
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| radioactive carbon dioxide (conversion of c as glucose to c as co2 indicates the metabolism...thus life |
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| a medium containing lauryl sulfate inhibits growth of g-pos bacteria.. theis medium is |
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| a medium that inhibits the growth of certain bacteria while permitting the growth of others is described as |
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| to look for bacteria that degrade petroleum, a culture med contining crude oil, sodium nitrate, phosphate buffer and magnesium sulfate is inoculated with soil.. this medium is |
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| if there is not a formula for crude oil, the medium would be described as |
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| what is the third step in binary fission |
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| inward growth of membrane |
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| which biosafety leve, has ... open lab benchtop, gloves, lab coat, and face prtotection |
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| psychrotrophic org are often responsible for food spoilage in refrigerated foods, t or f |
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| t or f... adding salts to a solution increases osmotic pressure and is used to preserve food |
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| microaerophiles do not require oxygen for growth t or f |
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| aerotolerant organisms can grow in the presence of oxygen because their cell wall protects them from toxic prod of oxygen... t or f |
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| microaerophiles do not require oxygen for growth |
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| in a direct microscopic cnt, dead cells are easily differenctiated from live cells t or f |
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| why is homemade potato salad refig overnight and served without any cooling on a warm day bad? |
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Definition
| lg volume of pot salad is probably never completely cooled before serving. second not keeping it cold the next day will allow more bactria to grow. |
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| what is the disadvantage of using turbidity to estimate the number of viable bact cells in a broth |
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Definition
| turbidity method counts dead celss as well as those that are viable and turbidity cannot be used on very dilute population |
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Term
| slower growing bact would have a ____ slope than those with a faster generation time |
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Definition
| greater slope (therefore; faster-growing bacteria would have a greateater slope than would those with a slw gen time |
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Term
| t or f, psychrotrophic org are often responsible for food spoilage in refrigerator |
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Definition
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| adding salts to a solution increases the osmotic press and is used as a preservative t or f |
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| microaerophiles do not require oxygen for growth |
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| aerotolerant org can grow in a the presence of oxygen because their cell wall protects them from toxic products of oxygen |
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| during the stationary phase, bacteria cont to undergo binary fission |
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| candle jars work well for cultivating anaerobic bacteria t or f |
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| when the spread plate tech is used to inoculate culture media some colonies form within the agar- t or f |
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| in a direct microscopic cnt, dead cells are easily diff from live t or f |
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| turbidity or cloudiness of a cell culture measured by spectophotometry is useful in detecting sm amt of microbial contamination in liq t or f |
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| cells growing in a rich med will have a steaper log phase slope than will those growing in a nutrient-poor med t or f |
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| shrinking of the cells cytoplasm in response to osmotic loss is called |
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| the enzyme ___ catalyzes the conversion of peroxide to water and oxygen... |
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| the ___ is the most common tech used to obtain pure cultures of microbes (3 words) |
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| bacterial growth refers to an increase in the _____ |
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Definition
| number of bacterial cells |
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Term
| an unknown org grows at the top of a tube of thioglycolate broth, the org is best described as..... for oxygen req |
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Definition
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| salt agar tends to inhibit the growth of most organisms except staph.. this is ____ media |
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Definition
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| in a ____ cells are added to melted agar |
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| a type of cell to cell communication that allows bact growing in biofilms to communicate and coordinate their activity is known as ____ |
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| in a pour pl tech, 32 bacterial colonies are counted on the 10 (-2) dilution of milk... how many bacteria were in the org milk |
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| infections concerd with biofilms ... the bacteria in biofilms are more likely to be __________ |
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| what tech will allow you to get isolated colonies on agar |
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Definition
| useing a streak plate technique to inocultae |
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Term
| a medium containing lauryl sulfate inhibits growth of g-pos bacteria.. this medium is ___- |
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| s-s agar inhibits g-pos bacteria. on this medium is ______ |
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Definition
| both selective and differential |
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| a bacterial species that grows fastest at 55c, which means it is a ______ |
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| a _____ would be the most practical way to determine the number of bacteria in cottage cheese |
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| nitrogen will be found in proteins, which include ____ |
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| describe binary fission steps |
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Definition
| after dna rep and cell elongation the membrane grow inward between the 2 new formed cells |
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| clostridium can be cultured in an anaerobic incubator or in the presence of atmospheric oxygen if thioglycolate is added to the nugrient broth the addition of thiog... makes the medium |
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| bacteria grown with a campypak that produces 5% co2 and 15% o2, this bacterium is a ____ |
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Definition
| microaerophile (atmosphere provided by the campypak has reduced lev of o2 perfect for microaerophile) |
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| bacteria that spoil food in the fridge are most likely _____, they prefer 20C |
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| if 52 bacterial colonies grow on agar inoculated with 1:1000 of hamburger hwo many bact originated |
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