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Micro CH 12
Disinfection/Sterilization
59
Biology
Undergraduate 2
03/25/2011

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Term
sterilization
Definition
killing or removal of all microorganisms in a material or on an object
Term
disinfection
Definition
reduction of the number of pathogenic microorganisms to the point where they no longer pose any danger of disease
Term
antiseptic
Definition
chemical agent that can safely be used externally on living tissue to destroy microorganisms or to inhibit their growth
Term
disinfectant
Definition
chemical agent used on inanimate objects to destroy microorganisms. most do not kill spores
Term
sanitizer
Definition
chemical agent typically used on food-handling equipment and eating utensils to reduce bacterial numbers so as to meet public health standards. may simply refer to thorough washing with only soap or detergent
Term
bacteriostatic agent
Definition
agent that inhibits growth of bacteria
Term
germicide
Definition
agent capable of killing microbes rapidly; some effectively kill certain microbes but inhibit others
Term
bacteriocide
Definition
agent that kills bacteria, most such agents do not kill spores
Term
viricide
Definition
agent that inactivates viruses
Term
fungicide
Definition
agent that kills fungi
Term
sporocide
Definition
agent that kills bacterial endospores or fungal spores
Term
action of soaps and detergents
Definition
lowers surface tension, makes microbes accessible to other agents
Term
actions of surfactants
Definition
dissolves lipids, disrupts membranes, denatures proteins, & inactivates enzymes in high concentrations
act as wetting agents in low concentrations
Term
actions of acids
Definition
lowers pH and denatures proteins
Term
action of alkalis
Definition
raises pH and denatures proteins
Term
actions of heavy metals
Definition
denatures proteins
Term
actions of halogens
Definition
oxidize cell components in absence of organic matter
Term
actions of phenols
Definition
disrupts membranes, denatures proteins, and inactivates enzymes; not impaired by organic matter
Term
actions of oxidizing agents
Definition
disrupts disulfide bonds in proteins
Term
action of alkylating agents
Definition
disrupts structure of protein and nucleic acids
Term
action of dyes
Definition
may interfere with replication or block cell wall synthesis
Term
uses of soaps/detergents
Definition
hand washing, laundry, sanitizing kitchen and dairy equipment
Term
uses of surfactants
Definition
cationic detergents used to sanitize utensils
anionic detergents used to launder clothes & clean household objects
quaternary ammonium chloride compounds are sometimes used in antiseptics on skin
Term
uses of acids
Definition
food preservation
Term
uses of alkalis
Definition
found in soaps
Term
uses of heavy metals
Definition
silver nitrate in baby's eyes to prevent gonococcal infections
mercury compounds to disinfect skin & innanimate objects
Term
uses of halogens
Definition
chlorine is used to kill pathogens in water and to disinfect utensils
iodine compounds are used as skin antiseptics
Term
uses of alcohols
Definition
isopropyl used to disinfect skin
ethylene glycol & propylene glycol used in aerosols
Term
uses of phenols
Definition
phenol: disinfect surfaces and destroy discarded cultures
amylphenol: destroys vegetative organisms & inactivates viruses
chlorhexidine gluconate: effective as a surgical scrub
Term
uses of oxidizing agents
Definition
hydrogen peroxide is used to clean puncture wounds, potassium permanganate to disinfect instruments
Term
uses of alkylating agents
Definition
formaldehyde used to inactivate viruses without destroying antigenic properties
glutaraldehyde to sterilize equipment
betapropiolactone to destroy hepatitis viruses
ethylene oxide to sterilize inanimate objects that would be harmed at high temps
Term
action of dry heat
Definition
denatures proteins
Term
action of moist heat
Definition
denatures proteins
Term
action of pasteurization
Definition
denatures proteins
Term
action of refrigeration
Definition
slows the rate of enzyme controlled reactions
Term
action of freezing
Definition
greatly slows the rate of most enzyme-controlled reactions
Term
action of drying
Definition
inhibits enzymes because of lack of water
Term
action of freeze-drying
Definition
dehydration inhibits enzymes
Term
action of UV light
Definition
denatures proteins and nucleic acids
Term
action of ionizing radiation
Definition
denatures proteins and nucleic acids
Term
action of microwave radiation
Definition
absorbs water molecules, then releases microwave energy to surroundings as heat
Term
action of strong visible light
Definition
oxidation of light-sensitive materials
Term
action of sonic & ultrasonic waves
Definition
cause cavitation
Term
action of filtration membranes
Definition
mechanically removes microbes
Term
action of osmotic pressure
Definition
removes water from microbes
Term
uses of dry heat
Definition
oven heat to sterilize glassware and metal
open flame to incinerate microorganisms
Term
uses of moist heat
Definition
autoclaving sterilizes hospital and lab equipment not damaged by heat and moisture
pressure cooking sterilizes canned foods
Term
uses of pasteurization
Definition
kills pathogens in milk, dairy, and beer
Term
uses of refrigeration
Definition
keeps fresh foods for a few days; does not kill most microorganisms
Term
uses of freezing
Definition
used to keep fresh food for several months; does not kill microorganisms; used with glycerol to preserve microorganisms
Term
uses of drying
Definition
used to preserve some fruits and vegetables, sometimes used with smoke to preserve sausages and fish
Term
uses of freeze drying
Definition
used to manufacture some instant coffees
used to preserve microorganisms for years
Term
uses of UV light
Definition
used to reduce number of microbes in air in operating rooms, animal rooms, and where cultures are transferred
Term
uses of ionizing radiation
Definition
used to sterilize plastics and pharmaceutical products used to preserve foods
Term
uses of microwave radiation
Definition
cannot be used reliably to destroy microbes except in special media-sterilizing equipment
Term
uses of strong visible light
Definition
can be used with dyes to destroy bacteria & viruses
may help sanitize clothing
Term
uses of sonic and ultrasonic waves
Definition
not a practical means of killing microbes
useful in fractionating and studying cell components
Term
uses of filtration membranes
Definition
used to sterilize media, pharm. products, and vitamins
used in manufacturing vaccines
used in sampling microbes in air & water
Term
uses of osmotic pressure
Definition
used to prevent spoilage of foods such as pickles and jellies
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