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Micro Bio University of Kansas: Dr Ford
Lecture 10-14
50
Biology
Undergraduate 2
12/09/2010

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Term

What is special about autotrophs?

 

Definition
First organism on earth. Pathway is used in all forms of life.
Term
What do humans use in the Electron transport chain, as a electron acceptor and an electron donor?
Definition
In electron transport cahin, humans use glucose as an electron donor and oxygen as an acceptor.
Term
Name autotroph electron donors?
Definition

Hydrogen gas-(H2)

Formic acid- CH2O

Ammonia-NH3

Nitrate-NO3

Hydrogen Sulfide-H2S

Term
Name autotroph electron acceptors?
Definition

Oxygen-O2

Sulfate-SO4

Nitrate-NO2

Nitrogen gas-N2

Term
Passing electrons to O2 is a favorable chemical process but requires what?
Definition
activation energy
Term
What are Electron transport chain proteins?
Definition

Enzymes that lower the energy barriers, alowing electrons to pass.

-Energy is released each time an electron passes through an enzyme.

-Some enzymes can use tha energy to move protons out of the cytoplasm.

 

Term

Where does the electron transport chain take place?

 

Definition
On the membrane of the mitochondria.
Term
What happens in the E.Coli Electron transport chain?
Definition

1) 2 electrons are passed from NADH to NDH-1(releasing 4 H+)

2) Electrons go from NDH-1 to Quinones(attaching 2 H+ to it)

3)Goes from Quinones to Cytochrome BO(realeasing 4 H+)

Term
What happens in the Mitochondrial ETC when NADH is used? How many complexes does it consist of?
Definition

It consists of 4 complexes. 

1)Complex 1 takes electrons from NADH and passe's them to Coenzyme Q(releasing 4 H+)

2)Coenzyme Q delivers electrons to complex 3(releasing 2H+)

3)electrons pass to cytochrome C (releasing 2 H+)

4)Cytochrome C moves it electrons to complex 4, which are then transfered to O2 making H2O (releasing 2H+)

Term
What happens in the Mitochondrial ETC when FADH2 is used?
Definition

1)FADH2 transfers its electrons to complex 2

2)Coenzyme Q picks up electrons and delivers them to complex 3(releasing 2H+)

3)Electrons from complex 3 to cytochrome C(releasing 2H+)

4)Cytochrome C moves electrons to complex 4

5)Complex 4 transfers electrons to Omaking water.(releasing 2H+)

Term
How many protons are made with NADH and FADH2? Where are they pumped?
Definition

NADH moves 10 protons into the periplasm.

FADH2 moves 6 protons into the periplasm,

Term
What is the overall equation of Cellular respiration? How does this relate to photosynthesis?
Definition

C6H12O6 + 6O2 -----> 6CO2 + 6H2O


Photosynthesis is just the reverse of cellular respiration.

Term
What bacteriocholorophyll do purple photosynthetic bacteria use?
Definition
Bacteriachlorophyll P870.
Term

In what spectrum do purple photosynthetic bacteria absorb light?

What are there electron donor and acceptor?

What do purple photosynthetic bacteria not produce from H2O?

Definition

-Purple photosynthetic bacteria absorb light in the infared spectrum. 

-P870 is both the electron donor and acceptor.

-They do not produce oxygen from H2O.

Term
Where does photosynthesis take place in purple photosynthetic bacteria?
Definition
It occurs on a membrane and P870 is located on photosystem 2.
Term
What happens during photosynthesis for purple photosynthetic bacteria?
Definition

1) light excites an electron on photosystem 2.

2) 2 electrons are donated to Quinone.

3)Quinone becomes Quinol(QH2)

4)Quinol transfers electrons to cytochrome BC(two H+ are released)

5) electrons transfer to cytochrome c and return to photosystem 2.

Term

What photosystems do cyanobacteria use?

What is there electron source?

Definition

-Photo-system 2(P680) and Photo-system 1(P700)

-They use H2O as an electron source.

-They produce O2 from H2O.

Term
How do Cyanobacteria carry out photosynthesis?
Definition

1) 4 photons of light take 4 electrons from two molecules of H2O(releasing 4 H+)

2) electrons transfer to two quinones.(becoming Quinol(QH2))

3) two quinols transfer electrons to cytochrome bf(releasing 4 H+)

4)Plastocyanin transfers electrons to photo-system 1(releasing 4 H+)

5) light excites the electrons enabling the transfer to ferredoxin and NADP+(making NADPH)

Term
What are the NADPH and ATP used for after cyanobacteria carry out photosynthesis?
Definition

The NADPH and ATP are used to make glucose.

The cost of one glucose molecules= 6CO2+ 18atp+ 12 NADPH

Term

Where does Glycolisis occur?

Where does the citric acid cycle occur?

Where does the electron transport chain take place?

 

Definition

Glycolisis occurs in the cytoplasm.

The citric acid cycle occurs in the matrix of the mitochondria.

The electron transport chain is on the inner membrane of the mitochondria.

Term
What are the reactants and products of glycolisis?
Definition

1 glucose molecule and 2 ATP are needed to carry out glycolysis.

2 NADH, 4 ATP and 2 pyruvate are the products.

Term
What are the steps of Glycolysis?
Definition

1)Hexokinase adds a phosphate to pyruvate.(1 atp used)

2)Glucose isomers move a C-O bond.

3)Phosphofructokinase phosphorolates becoming 1,6 diphosphate.(1 atp used)

4) Aldolase splits the molecule creating DHAP and G3P

5)Triosphosphate isomers convert DHAP to G3P(2 G3P exist)

6)G3P dehydrogenase oxidizes the 1 carbon(creating 2 NADH)

7)Phophoglycerate kinase removes a phosphate group.(2 atp made)

8)Phosphoglyvertomutase moves the remaining phosphate to the middle carbon

9)enolase removes H2O

10)Pyruvate kinases remove a phosphate and add it to ADP(2 atp made)

Term
What are some Microbial electron acceptors?
Definition

Sulfate-SO4-

Nitrate-NO3

Sulfur-SO2

CO2

Term
What is the difference between aerobic respiration and anaerobic in terms of electrons?
Definition

In aerobic respiration electrons are passed to O2,

while in fermentation(anaerobic) they are passed to endogenous organic molecules.

Term
How is lactic acid formed in lactic acid fermentation? What organisms carry out this process?
Definition
Lactic acid is produce by the reduction of pyruvate. This process is used by animals muscles and microbes. The lactic acid will be oxidized back to pyruvate in animal cells.
Term
What happens to pyruvate in ethanol fermentation?
Definition
The pyruvate is decarboxylated to form acetaldehyde. The acetaldehyde is reduced to form ethanol.
Term
What are Saccharomyces?
Definition
Saccharomyces are a species of yeast taht are used to make beer. They are used for ethanol fermentation and baking. (They are better able to withstand ethanol then bacteria.)
Term
Where does the citric acid cycle occur? What occurs before it?
Definition

The citris acid cycle occurs in the matrix of the mitochondria. It occurs right after the transisition step.

Transition step reactants: 2 pyruvate go in

Products: 2 acetyl Co-enzyme A, 2 Co2, and 2 NADH  are made.

Term
How is pyruvate made into Acetyl Co-enzyme A?
Definition
O=C=O is removed from pyruvate and a Sulfur-(Co-enzyme A) bonds making Acetyl CoA.
Term
What are the products and reactants for Citric Acid Cylce?
Definition

reactants: 2 acetyl CO-A

products: 4 CO2,2 ATP, 6 NADH, 2 FADH2

 

note: during the cycle 2 Carbon will have to cycle through multiple times to be completely degraded.

Term
For what are Citric Acid Cycle intermediates used for?
Definition

Citric acid cycle intermediates are used in other processes.

-alpha ketoglutarate is used in amino acids.

-Oxaloacetate is used for amino acid and nucleotides.

(both intermeidates of Citric acid cycle)

Term
What are Anapleric reactions?
Definition
Anapleric reations make new intermediates for metabolic pathways.
Term
____ nitrogen is required for the synthesis of amino acids. ____% of the atmosphere is nitrogen but N2. Nitrogen can only be reduced to ammonia by bacteria using ______.  _______ in optimal conditions will need______ATP and _____NADH to make N2 into ammonia and under average conditions needs _____ATP and ___ NADH. The ______enzyme can only function in a ____ free environment.
Definition
Reduced nitrogen is required for the synthesis of amino acids. 70% of the atmosphere is nitrogen but N2. Nitrogen can only be reduced to ammonia by bacteria using NitrogenaseNitrogenase in optimal conditions will need 12 ATP and 3 NADH to make N2 into ammonia and under average conditions needs 16 ATP and 4 NADH. The Nitrogenase enzyme can only function in a oxygen free environment.
Term
Bacteria species that are crucial to Nitrogen fixation?
Definition

Azotobacter=live in soil

Rhizobium=infect plant roots

Nodules=Tumors in plant roots that form around rhizobium

Term
What do Rhizobaum do?
Definition
There is  a mutualistic symbiosis with legumes. The plant provides the ATP and NADH while the bacteria provide the nitrogen.(this is important in farming)
Term
What enzymes are key to Nitrogen Assimmilation? What does Nitrogen Assimilation do?
Definition

Alanine Dehydrogenase= which adds ammonia to pyruvate

Glutatmate= adds ammonia to a-Ketogluterate

 

Nitrogen Assimilation is the process in which Nitrogen are added to amino acids.

Term
What are Transminases?
Definition
An enzyme that moves amino acid groups. They are used to make other amino acids by moving groups of glutamate and alanine onto other amino acids.
Term
Molecules and energy?
Definition
Molecules have kinetic energy and are constantly in motion. Heating speeds this up and cooling slows them down.
Term
What is Diffusion?
Definition
Diffusion= Movement of a substance from an area of high concentration to a lower concentration until evenly dispersed.
Term
How does membrane transport work as far as what is allowed through?
Definition

Membranes are selectively permeable. Some molecules pass through easily while other cannot.

-Non polar molecules can pass through membranes.

-Polar and ionic compounds cannot pass though membranes with out assistance.

Term
What is passive transport?
Definition
Diffusion occurs across a membrane. No energy is used.
Term
What is facilitated diffusion?
Definition
Polar and ionic compounds cannot pass through a phospholipid membrane. The use of special proteins such as channel proteins are needed.
Term
What is active transport?
Definition

hen a cell uses energy to move a solute against a concentration gradient.

-Can use ATP to transport compounds

Term
What is antiporter? What is symporter?
Definition

Antiporter is one molecule moves in while another moves out.

Symporter is moving two molecules simultaneously.

Term
What is Gluconeogenesis?
Definition
It is the reverse fo glycolisis. The process of using pyruvate to make glucose. The first step is energetically expensive.
Term
What are the steps of Gluconeogenesis?
Definition

1)use ATP to add CO2 to pyruvate.(forms Oxaloacetate)

2)Phosphate from GTP binds to the middle bond, releasing CO2.(forming Phosphenol Pyruvate)

3) Glycolisis and Gluconeogenisis enzymes are inhibited by the amount of glucose. They also inhibit ATP levels.

-glucose acts as a inhibitor for point of control enzymes

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