Term
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Definition
- tests for citrase activity
- citrate --> oxaloacetate via citrase
- color change from green --> blue (acidic --> basic)
- blue = Positive for citrase activity |
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Term
| Sulfide Indole Motility (SIM) Agar |
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Definition
- tests for H2S, motility, indole
- H2S production characterized by formation of black ppt.
- motility: if turbid, yes. if not, no
- indole production: add Kovac's reagent; if red ring forms, then (+) for indole (tryptophan --> indole by bacterial enzyme tryptophanase) |
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Term
| Motility Indole Ornithene (MIO) Agar |
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Definition
- tests for indole, motility, and ornithene decarboxylation
- motility: measured by turbidity
-ornithene decarboxylation: if purple, then (+) (BASIC); if yellow, (-) acidic
- Indicator = brom-cresol purple
- indole = red ring means (+) for indole |
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Term
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Definition
- tests for urease activity
- (NH2)2CO (urea) --> CO2 + H2O + NH3 (ammonia)
- color change from yellow to bright pink indicates that urease is present
- yellow (acidic) --> pink (basic) |
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Term
| Carbohydrate Broths (glucose, sucrose/dextrose/lactose) |
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Definition
- tests for carbohydrate fermentation into acidic end products: CHO --> OH- (acidic) + CO2
- indicator = phenol red
- yellow = (+) for acidic end products, red = (-) |
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Term
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Definition
- tests for ACIDIC END PRODUCTS of glucose fermentation
- glucose (ferm)--> OH- (acidic) + CO2
- red = (+) for acidic end products |
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Term
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Definition
- tests for NONACIDIC END PRODUCTS of glucose fermentation
- glucose (utilization) --> non-acidic end products
- pink = (+) for non-acidic end products, yellow = (-) |
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Term
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Definition
- tests for nitrate reductase activity
- NO3(2-) (nitrate) --> NO2(-) --> N2(g)
- test by adding reagents A & B --> if color change, (+) for nitrate reductase; if no color change, add reagent C(zinc)--> if color change then, (-) for nitrate reductase |
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