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Mcvay Microbiology
Chapter 5 and half of Chapter 21 Quiz 2
69
Biology
Undergraduate 2
02/15/2011

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Cards

Term
Control mechanism can be what?
Definition
Physical, chemical, or a combination of  both
Term
What are physical methods?
Definition

  • Heat
  • Irradiation
  • Filtration
  • Mechanical removal

Term

What are Chemical methods?

 

Definition

Chemical method use  a variety of anti microbial chemical which  depends on

1. the circumstances 

2. the degree of control required

Term
Reasons for  controling bacteria growth are what? 
Definition

  • infectious disease
  • food spoilage
  • transmission of food- borne illness

Term
Sterilization 
Definition

 

  • The removal of all  microorganisms
  • completely free of microcrobes, viruses, and endospores

 

Term
How is sterilization achieved?
Definition

 

  • Filitration
  • Heat 
  • irradition 

Physical methods!

 

Term
Disinfection
Definition

  • eliminate most pathogens
  •  BUT some viable microbes do exist 

Term
What are disinfectants used on?
Definition

  • inanimate objects and surface

Term
What are antiseptics used on?
Definition
Antiseptics are used on living tissue.
Term
Pasterurization
Definition
 heat treatment used to redue organisms that cause food spoilage.
Term
Decontaminations
Definition
Treatment used to reduce pathogens to a safe level
Term
Degerming
Definition
Used to decrease the number of microbes in an area -- especially on the skin
Term
Preservation
Definition

  • Used to delay spoilage of perishable items
  • Uses the additions of growth- inhibiting ingredients

Term
Batericidal
Definition

  • agents that kill vegetatative cell but not endospres

Term
Bacteriostatic
Definition

  • Agents that prevent or retard the multiplication of bacteria but do not necessarily kill cells

Term
Sterilants and Sporocides
Definition
Disinfectants that kills endodpores
Term
In daily life what is the most imprtant step to achieveing microbial control?
Definition
Handwashing
Term
Soap acts as what and aids? 
Definition
It acts as a wetting agent and aids in mechanical removal of microorganisms by removing  numerous organisms from the outer layer of the skin.
Term
Why is not normal flora not affected by hand washing?
Definition
Because it  in deeper layers of the skin
Term
Pathogens are most likely to be found in where? 
Definition
Hospitals
Term
Why do numerous organism develop  antimicrobial resistance?
Definition
Because they are exposed to high concentrations of antibiotics
Term
What do insturmnet go through in order  to avoid intorducing infection deep tissue?
Definition
Sterilized
Term
In the  microbiology laboratory,  what eliminates unwanted organism in growth?
Definition
Assptic technique and sterile media
Term
What is used to prevent contamination in Microbiology lab?
Definition
Biological safety cabinets
Term
Food quality is enhanced through the convention of What?
Definition
Microbial growth and contamination
Term
In food how is the prevention of microbial growth and contamination achieved? 
Definition
through physical removal and chemical destroying of the organisms
Term
True or false irradiation can treat some food.
Definition
True
Term
What ensures that drinking water is safe?
Definition
Water treatment
Term
What is generally used to disinfect water?
Definition
Chlorine
Term
What is one of the organisms that is not easily eliminated with chlorination?
Definition
cryptosporidium sp.
Term
The selection of an antimicrobial procedure depends on what factors
Definition

  1. Type microbe
  2. Extent of contaminantion
  3. enviroment
  4. Risk of infection
  5. Composition of infected item

Term

Bacterial endospores

(resistant microbes)

Definition
Resistant to heat, drying and numerous chemicals
Term
Protozoan cyst and oocytes
Definition
Generally excreted in feces and causes diarrheal disease
Term
Mycobaterium  species 
Definition
Cell wall structure initiates resitance
Term
Pseudomonas
Definition
Can  grow in presence of many chemical disinfectants
Term
Viruses
Definition
More resistant to chemical killing
Term
The time it takes to kill a colonyis directly  conected to..
Definition
the  population size
Term
D value
Definition
Time required to kill  90%  of the population  under specific conditions
Term
What decreases time to reach disinfection or sterilization?
Definition
Washing
Term
What influences the effectiveness of antimicrobial?
Definition
 enviromental conditions
Term
What  specific  things can increase or decrease effectiveness?
Definition

  1. PH
  2. temperature 
  3. presence of organic materials

Term
How does organic material decrease effectiveness?
Definition
it can interfer with the penetration of the antimicrobial agent
Term
How are medical items catergorized ?
Definition

According  to their potential risk of disease transmission

 

Term
Critical items are  needle and scalpels. Why?
Definition
Because they come in contact with the body tissue
Term
Semi critical  medical items do what?
Definition

Come in contact with mucous membrane but do not penetrate

Ex :  an endoscope.

Term
Non crictical items
Definition

 Items that contact unbroken skin only and show little risk of transmission

Ex: stethoscope

Term
Why is composition important?
Definition
Because some methods are inapproriate for certain items
Term
What is the most useful for microbial control and why?
Definition
Heat treatment is because it is fast reliable, safe and inexpensive.
Term
Heat methods
Definition
Moist and dry heat
Term
Moist heat breaks down in to
Definition

Boiling 

Pasturization

Pressurized steam

Term
Boiling 
Definition
destroys most microorganisms and viruses. BUT IS NOT an effective means of sterilization because it does not destroy endospores.
Term
Pasteurization
Definition
Does NOT sterilize but significantly reduces organisms. It is used to increase the shelf life of food
Term
What is HTST?
Definition
High Temp Short time is when an organism is heated to  72 C and held for 15 seconds
Term
Ultra- high temperature 
Definition
UHT is when the temp is held at 140-150C and held for several seconds and then cooled rapidly
Term
Pressured steam 
Definition
The preferred method of sterilization. 
Term
What temp and time is used for pressured steam sterilization
Definition
121C and 15 psi in 15 min
Term
WHat does flash auto clave sterilize at?
Definition

135 C  

15ps

3 min

 

Term
When are Prion ( abnormal cellular protien) destroyed? 
Definition

132C 

15psi

4.5 hours

Term
Which is better dry heat or moist heat? Why? 
Definition

moist because dry heat  requires longer time with higer tempsQ

 200cFOR 1.5 HOURS

 

Term
Incineration 
Definition
method of dry heat sterilization that oxidizes the cell into ashes. 
Term
Heat sensitive material must undergo what type of methods?
Definition

  • filtration
  • irradiation
  • High-pressure treatment

Term

radiation

Definition
uses energy released from waves based on the wavelength and frequency
Term
radiation can break down into two categories
Definition

  1. ionizing
  2. non ionizing

Term
ionizing radition
Definition
strips electrons from atoms
Term
What does ionizing radiation damage?
Definition
Dna and potentially the plasma membrane.
Term
in ionizing radiation endosprores are completely ridded of.
Definition
False
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