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MBG 2400 LEE Concept #3 RNAi
RNAi, interference
11
Biology
Undergraduate 2
10/14/2013

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Term
How long are miRNA/siRNA/piRNA?
Definition
20-30 nucleotides
Term
What other forms of RNA are there, in addition to mRNA, tRNA and rRNA?
Definition
micro RNA
small interfering RNA
piwi interacting RNA
Term
Are siRNA & miRNA single or double stranded?
Definition
double stranded
Term
Are piRNA single or double stranded?
Definition
single stranded
Term
True or False
miRNA acts in the cytoplasm to suppress transcription
Definition
False
miRNA acts in the nucleus to silence transcription
Term
Where does siRNA act? What does siRNA do?
Definition
In the cytoplasm
Degrades mRNA & inhibits translation
Term
Where does miRNA act and what does it do?
Definition
In the nucleus
Silences transcription & remodels chromatin
Term
What complex works in conjunction with siRNA?
Definition
RISC
Term
What complex works in conjunction with miRNA?
Definition
RITS
Term
What is RNAi?
Definition
RNA interference
Term
How are small RNA fragments formed?
Definition
-introduction of a small segment of DNA into the cell
-this segment is transcribed in both directions
-the 2 RNA strands that are produced find each other
-this is bad, cell recognizes this
-machinery cuts the double stranded RNA fragment
-pieces of these migrate to a DNA strand and bind to it, blocking transcription
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