Term
|
Definition
| Def: Nucleotide sequences from two different sources, often two different species, are combined in vitro into the same DNA molecule |
|
|
Term
|
Definition
| Def: small circular DNA molecules that replicate separately from the bacterial chromosome |
|
|
Term
|
Definition
| Def: an enzyme that seals the bonds between restriction fragments |
|
|
Term
|
Definition
| A DNA molecule that can carry foreign DNA into a host cell and replicate there |
|
|
Term
|
Definition
Def: The collection of recombinant vector clones produced by cloning DNA fragments from an entire genome
- Made using bacteria
- When made using bacteriophages, stored as a collection of phage clones |
|
|
Term
| Bacterial artificial chromosome (BAC) |
|
Definition
- Large plasmid that has been trimmed down and can carry a large DNA insert
- Another type of vector used in DNA library construction |
|
|
Term
| complementary DNA library |
|
Definition
- Library made by cloning DNA made in vitro by reverse transcription of all mRNA produced by a cell
- Represents only part of the genome (only part that is transcribed) |
|
|
Term
Nucleic acid probe
Nucleic acid hybridization |
|
Definition
- Tagged clone carrying complimentary nucleotide sequence to gene of interest
- Process of using nucleic acid probe |
|
|
Term
| Yeast Artificial Chromosomes (YACs) |
|
Definition
- Cultured yeast cells as host cells of gene expression
- Helps avoid prokaryotic expression system problems
- Behave normally in mitosis and can carry more DNA than a plasmid
- Can provide post-translational modifications that many proteins require |
|
|
