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Lecture 4
Biology of the Cell
47
Biology
Undergraduate 2
09/13/2010

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Term
describe ways in which cells differ from each other
Definition

- shape

- function

 

Term

be able to draw and label a cell. including:

- plasma membrane

- nucleus

- endoplasmic reticulum

- golgi apparatus

- mitochondria

Definition
Term
describe the architecture of plasma membrane.
Definition

- acts like sausage, keeps stuff in cell

- lipids- basic framework of the cell- bilayer of the phospholipid.

- permeable to water, oxygen, co2, but not to sugars, amino acids, ions.-- can get through with special channels.

- hydrophobic tails, hydrophilic heads.

- contains cholesterol- how stiff it is.

- contains proteins.- intergral, peripheral.

- contain glycoprotiens

- glycoprotein, glycolipids- cell recognition.

Term
name the 11 organelles (most important ones)
Definition

- endoplasmic reticulum (smooth and rough)

- ribosomes

- golgi apparatus

- mitochondria

- lysosomes

- peroxisomes

- centrosome

- cilia and flagella

- vesicles

- microfilaments and microtubules

- inclusions.

Term
describe the function of the endoplasmic reticulum (smooth, and rough).
Definition

smooth endoplasmic- helps synthesize proteins, and lipids.- no ribosomes attached to it.

 

rough endoplasmic- synthesize proteins and lipids, with ribosomes attached to it. 

Term
what is the function of ribosomes.
Definition
ribosomes- protein synthesis- help connect amino acids together. 
Term
what is the function of the golgi apparatus?
Definition
- packages, modifies, and secretes newly synthesized proteins that need to be secreted out of the cell. 
Term
what is the function of mitochondria
Definition
- use oxygen to oxidize glucose and fatty acids to make ATP. basically act as the energy source for the cell also plays a role in apoptosis. 
Term
what is the function of lysosomes?
Definition
membrane bound organelles that contain powerful enzymes to digest, and break down protiens such as bacteria, or foreign particles. 
Term

what is the function peroxisomes?

 

Definition
bags of enzymes--to break down fatty acids, synthesize cholesterol, make part of the mylelin sheath. 
Term
what is the function of centrosomes?
Definition
area where microtubules are produced.
Term
what is the function of the cilia and flagella?
Definition
plays an active part in cell movement. allow the cell to be motile. (move actively,and spontaneously.)
Term
function of vesicles
Definition

- endo/exocytosis

- contain and move various substances- ex hormones and neurotransmitters.

Term
function of micro filaments and microtubules.
Definition

- involved with mobility

- made of protein

- help move organelles in the cell interior.

Term
function of inclusions
Definition
ASK, TOTALLY DONT GET THIS.
Term
name 3 types of junctions between certain cells. what is a junction?
Definition

substances that connect cells together.

 

1. tight junctions

2. demosomes

3. gap junctions

 

Term
describe demosomes.
Definition
spot-welds between adjacent cells in skin.
Term
describe gap junctions
Definition
couple cells electrically and chemically.
Term
describe the nuclues and its structure. what are the 4 parts of the nucleus?
Definition

- located center of cell

- contains all the DNA of cell

- made up the following:

- nuclear envelope- has nuclear pores- allowd the RNA to leave the nucleus, and the protiens to leave the cytoplasm to get into the nucleus.

- nucleolus- ribosomes synthesized

- chromatin- consists of DNA and protiens.- DNA packaged into chromosome- then condenses and divides.

- nuclear matrix- contians molecules for gene expression.

 

Term

what is chromatin, and a chromosome?

 

Definition

chromatin- DNA molecules associated with protiens. long molecules- packed into a separate chromosome.

 

chromosome- condense, allows division, consists of twinned chormatids

Term
what are 3 ways substances can pass through a cell while NOT USING energy?
Definition

1. diffusion

2. osmosis

3. facilitated diffusion

Term
describe diffusion
Definition

- movement of substances from higher concentrations to lower concentrations.

 

 

Term
decribe facilitaed diffusion
Definition
- when the substances are insoluble thery can pass trhough the cell using carrier protiens.
Term
describe osmosis
Definition

special case of diffusion involving water

goes throught he layer using aquaporins.

 

goes from high to low concentration.

Term
list 3 ways substances can go through cells USING ENERGY.
Definition

1. Active transport

2. Endocytosis

3. Exocytosis

 

Term
Describe Active Transport
Definition

- pass from regions of low concentration to high concentraion.- against gradient

- consumes a lot of energy.

- sometimes protiens are involved, but always use ATP.- the proteins and ATP are coupled.

Term
Describe the different types of endocytosis.
Definition

endocytosis- push part of the substance into a vesicle and the moves the the membrane and makes lets it go forming an intracellular vesicle.

 

1. Phagocytosis- engulfs particles such as bacteria.- releass superoxide that kills the bacteria. then fuses with lysosomes to form phagocytosis.

 

2. receptor- mediated endocytosis- membrance contains protiens that act as receptors for foreign molecules. pinch to the foreigners and then make an intracellular vesicle.

 

3. pinocytosis- enclose the substance into a tiny droplet.

Term
define Exocytosis.
Definition
- packages something in the cell into a vesicle, then fuses with the membrane and releases contents
Term
What are the 4 stages of mitosis?
Definition

- (interphase- NOT INCLUDED)

- prophase

- metaphase

- anaphase

- telophase

 

Instant Pudding Made Any Time.

 

Term
describe prophase.
Definition

- 46 chromosomes visible as chromatids.

- duplicated and joined at centromere

- move to opposite sides of the nucleus

- mitotic spindle assembled attached to  two poles.

- nuclear envelope disappears.

Term
describe metaphase.
Definition

- line up at the equator.

- spindle fibers are attached to the centromeres

Term
describe anaphase.
Definition

- separate the sister chromatids.

- do to opp. poles

- now starting- CYTOPLASMIC DIVISION (cytokinesis.)

Term
describe telophase.
Definition

- nuclear membranes begin to reform

- cytokinesis is complete.

Term

why do we need cell cycle checkpoints?

 

Definition

- provide time for DNA repair, and DNA editing, also helps to prevent cancer.

 

 

Term
where do cell check points occur?
Definition

- at G1 phase

- G2 phase

- M- phase.

 

Term
what is differentiation, and gene expression?
Definition

differentiation- when a cell decides what "path" it wants to take and begins to grow into that specialized cell.

 

gene expression- what the cell turns out to be- genetically. like what features it has, and stuff. (?)- CHECK THIS. 

Term
describe a tumor suppressor gene. describe a proto-oncogene.
Definition

tumor suppressor gene- INACTIVATION/LOSS of this gene- CAUSES CANCER.- there is one, if a 2nd one is inactivated= cancer.

 

proto-oncogene- ACTIVATION of this gene-CAUSES CANCER. 

Term
how do tumor suppressor genes work?
Definition

- produce proteins- cell cycle determinants.

- arrest cell division- usually in G1 phase.

- cell time for DNA to repair DNA.

- correct errors from replication.

 

 

Term
define apoptosis.
Definition

- during embryotic development- unwanted populations- selectively killed.

 

- cancer- from failure of apoptosis.

- or abnormal apoptosis- mutations.

 

DURING:

- cell shrinks and rounds up

- DNA break, chromatin condenses.

- nucleus breaks-- condensed chromatin.

- cell "blebs" developed on surface, break into membrane bound vesicles.

- eaten by macrophages.

- use caspases-- mitochondria plays key role in this.

 

Term
define caspases.
Definition

- proteases

- break down cellular proteins, activate enzumes to break down DNA.

- fragments into large vesicles- eaten by macrophages-- prevent inflammation.

Term
define necrosis.
Definition

- diff from apoptosis--> ACCIDENTAL cell death.

- suffer from severe damage and sudden injury.

 

steps:

- cell and mitochondria change shape- swell and lose function.

- membrane breaks down

- cells and organelles undergo lysis.

- substances released- cause inflammation.

Term
describe nuclear transfer cloning.
Definition

- oocyte taken- nucleus removed. (enucleated)

- fused with other nucleus- using electric pulses.

- causes the egg to be reprogrammed and start again.

- cell begins to divide- develops into an animal of the adult.

 

Term
why should we clone?
Definition

1. makes them have specific traits we want

2. use animal organs for transplanting.

3. get animals to secrete human proteins- help the sick. "pharm" animals.

4. repair human cells-- use stem cells can make them ANYTHING.

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