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Lecture 29 (03/19/14)
Brock's Microoorganisms
25
Microbiology
Undergraduate 3
04/07/2014

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Term
signal transduction
Definition
signal is external and detected by sensor that transmits the signal to the regulatory machinery
Term
two-component regulatory systems
Definition
most signal transduction systems have two parts and are called this

1. specific sensor kinase protein
2. response regulator protein
Term
kinase
Definition
enzyme that phosphorylates compounds using PO4 from ATP
Term
histidine kinases
Definition
sensor kinase that detects signals from the environment and phosphorylates itself using a specific histidine residue
Term
regulator protein
Definition
typically DNA-binding protein that regulates transcription after having PO4 transferred to it from a sensor kinase
Term
phosphatase
Definition
protein that removes PO4 from the response regulator protein (reseting the regulatory system)
Term
chemotaxis
Definition
movement towards or away from a chemical
Term
1. response to signal
2. controlling flagellar rotation
3. adaptation
Definition
three steps of chemotaxis
Term
methyl-accepting chemotaxis proteins (MCPs)
Definition
sensory transmembrane proteins in the cytoplasmic membrane that sense the presence of a chemical and bind to it
Term
CheA
Definition
sensor kinase protein (cytoplasmic) that becomes phosphorylated

rate of phosphorylation is increased by repellant ; decreased by attractant
Term
CheY
Definition
response regulator protein that controls flagellar rotation

accepts phosphate from CheA
Term
CheA and CheW
Definition
What are the proteins that MCP (methyl-accepting chemotaxis protein) contacts in the cytoplasm?
Term
counterclockwise- run
clockwise- tumble
Definition
clockwise and counterclockwise flagellar rotation cause what movement
Term
CheZ
Definition
protein that dephosphorylates CheY allowing cell to run
Term
adaptation
Definition
process of resetting chemotaxis system, uses feedback loop to stop responding
Term
CheB
Definition
removes methyl groups from MCPs

it works faster when phosphorylated
Term
CheR
Definition
methylates MCP at a constant rate
Term
Quorum sensing
Definition
mechanism to evaluate the density of cells of the same kind, asses population density
Term
autoinducer
Definition
specific signal molecule synthesized by the participating organism that diffuses across the cell envelope in both directions

will reach a high concentration if there are many other cells nearby

binds to activator protein and triggers transcription of quorum-dependent genes
Term
Acyl homoserine lactones (AHLs)
autoinducer 2
short peptides
Definition
The 3 classes of autoinducers are ___________
Term
stringent response
Definition
global control mechanism triggered by amino acid starvation or other stressors
Term
guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp)

AKA alarmones
Definition
nucleotides that regulate the stringent response and accumulate rapidly during amino acid starvation
Term
Alarmones
Definition
pppGpp and ppGpp
Term
RelA
Definition
protein that synthesizes alarm ones from ATP
Term
heat shock proteins
Definition
proteins made by heat stressed cells to help counteract the damage caused by heat and help a cell to recover from temperature stress
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