Term
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Definition
| zone of unwound DNA where replication occurs |
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Term
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Definition
enzyme that uses ATP to unwind the double-stranded DNA to expose short-single stranded regions
advances the replication fork |
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Term
| single-strand binding protein |
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Definition
| protein that stabilizes separated DNA to keep it from reforming double-stranded DNA during replication |
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Term
| origin of replication (oriC) |
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Definition
| single location on the chromosome where replication is initiated in bacteria |
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Term
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Definition
| replicating DNA that forms continuously |
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Term
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Definition
| replicating DNA that forms discontinuously and requires multiple RNA primers |
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Term
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Definition
| short segments that make up lagging strand |
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Term
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Definition
| enzyme that removes RNA primer and replaces it with DNA |
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Term
| DNA Polymerase III (Pol III) |
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Definition
several proteins including sliding clamp and clamp loader
adds ~1000 nucleotides per second |
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Term
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Definition
| enzyme that creates final phosphodiester bonds between nucleotides |
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Term
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Definition
| characteristic shape formed during bidirectional replication of bacterial chromosome from OriC |
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Term
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Definition
large complex of proteins involved in the replication fork
DNA moves through this complex |
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Term
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Definition
| enzymes that detect errors/mismatches in base pairs based on incorrect H bonding |
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Term
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Definition
| removes incorrect bases during replication |
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Term
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Definition
| proteins that recognize termination sites in DNA sequences |
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Term
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Definition
| sites on chromosome that signal end of replication |
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Term
topoisomerase IV
and possible FtsZ |
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Definition
| links two circles of DNA together during termination |
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Term
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Definition
| DNA replication in a test tube |
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Term
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Definition
| process by which PCR creates up to a billion copies of specific genes or DNA segments |
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Term
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Definition
| gene or DNA segment desired for PCR replication |
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Term
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Definition
| entire DNA molecule used in PCR |
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Term
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Definition
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