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Lecture 14
Exam 2
11
Microbiology
Undergraduate 4
10/18/2010

Additional Microbiology Flashcards

 


 

Cards

Term
generation time
Definition
The time it takes for a cell to double (in both mass and
number)
Term

review all the math in lecture 14 (bring calc to exam)

be able to calculate generation times and growth rate constants

Definition
yahhh bitch, yahhh!
Term
growth rate constant
Definition
k = log N - logN0
Term
batch culture
Definition
A culture growing in an enclosed vessel such
as a tube or flask where exponential growth
cannot continue indefinitely
Term
inoculum
Definition
a substance (a virus or toxin or immune serum) that is introduced into the body to produce or increase immunity to a particular disease
Term
Liebig’s law of the minimum
Definition
states that growth is controlled not by the total of resources available, but by the scarcest resource (limiting factor).
Term
dilution rate
Definition
  • can be the same as growth rate in a continuous culture
  • can be controlled by adjusting the
    flow rate of fresh medium containing limiting amounts of growth factor
Term
washout
Definition
the point where growth can not balance dilution because dilution rate is too large (growth rate doesn't equal dilution rate)
Term

VBNC

 

Definition

Viable, but not culturable

  • During stationary-death phases, some microorganisms (Gram negatives in particular) will gradually lose their ability to recover and reproduce when returned to standard nutrient conditions
  • These cells have metabolic activity and are structurally VBNC intact
  • May be a survival mechanism – a quiescent state analogous to the endospore (but not heat stable)
Term
how can growth factor bioassays be used to measure concentrations of certain vitamins
Definition
  • by measuring the growth rate or growth yield of a fastidious organism in a defined medium containing the food sample as the sole vitamin source and all other essential nutrients provided in excess.
  • Microbe can’t make the vitamin so it must be present in the culture medium for the organism to grow. The growth response is proportional to the vitamin concentration in the medium (sole source from the food sample).
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