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Microbiology
06/07/2010

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Cards

Term

why is the coagulase test the definitive

test for staph aures?

Definition

out of all the staphs that colonize humans aures

is the onlly one that is coagulase positive

Term
what is the role of coagulase in staph aures
Definition

staph uses it to form the fibrin clot for a

barrier against immune system

Term
what is the role of alpha toxin in staph aures?555 Pico Ave; Long Beach, CA 90802
Definition
causes cell lysis of rbc
Term
what are nosocomial infections?
Definition
hospital infections
Term
differentiate between alpha and beta hemolysis
Definition

alpha is partial hemolysis and will look green

beta is complete and there will be a clear halo

Term

what is rebecca lancefields contribution

to the study of strep

Definition
she divided it based on serological groups
Term
on blood agar what type of hemolysis would be expected from someone with streptococcal pharyngitis
Definition
beta hemoysis
Term
what causes streptococcal pharyngitis?
Definition
strep pyrogenes
Term

what are three tests used to differentiate

strep pyogenes and agalactaie?

Definition

Bacitracin suceptibility pygenes is agalactaie isnt

camp reaction agalactaie pos pyogenes neg

lancefield grouppyogenes A agalactiae B

 

Term
name two tests that are useful for diferentiating pnuemococci from oral viridians streptococci
Definition

optochin pnuemococci are susceptible viridians not

bile solubility pnuemococci are positive,viridians neg

Term
what test can be performed to differentiate between enterococci and other group d streptococci
Definition
6.5% nacl broth enterobacter+ other group d-
Term
what is the function of bacterial exoenzymes?
Definition
they breakdown macromolecules into smaller molecules that can be takin into the cell body and used as metabolites
Term
how are the end products of biochemical reactions typically detected in the broth or agar
Definition
detected with a ph indicator such as phenol red
Term

name two tests for reactions that

produce acid end products

Definition
KIA and fermentation of carbohydrates
Term

name two tests for reactions that

produce alkaline end products

Definition
Hydrolysis and litmus milk
Term

what types of gasses can be produced

as aresult of sugar fermentation?

how are they detected?

Definition

Co2

in a durham tube

Term

name a nonfermentative test in which gas

production indicates a positive test result

Definition
Nitrate reduction
Term
how do the methods for the detection of hydrolysis of starch,casein,and triglycerides differ in these three tests
Definition

for starch you must add iodine a clear halo is +

casein should produce a clear halo in milk for +

Triglycerides should turn dark blue

Term
in addition to the casein hydrolysis test whhat are two other tests that demonstrate hydrolysis of proteins or proteolytic digestion
Definition

cystein to hydrogen sulfide

tryptohan to indole

litmus milk

Term

what media do you use for a

2,3-butanediol fermentation test?

Definition
MR-VP broth
Term

what media do you use for a

carbohydrate fermentation test?

Definition
Kligers iron and phenol red lactose
Term

what media do you use in a

casein hydrolisis test?

Definition
skim mik agar
Term

what media do you use in a

citrate utilization test

Definition
simmons citrate agar
Term

what media do you use in a hydrogen

sulfide production test?

Definition
kligers iron agar and SIM medium
Term

what media would you use for a

mixed-acid fermentation test

Definition
MR-VP broth
Term

what media would use for a

triglyceride hydrolysis test?

Definition
spirit blue agar
Term

what media would you use for a

tryptophan degredation test

Definition
SIM media
Term
What reagent would you use in a 2,3-butanediol fermentation test?
Definition
alpha-napthol
Term
what reagent would you use in a catalase test?
Definition
hydrogen peroxide
Term

what reagent would you use in a

mixed acid fermentation test

Definition
methyl red
Term

what reagents would you use in a

nitrate reduction test

Definition

dimethyl-alpha-naphthylamine

and sulfanilic acid

Term
what reagent would you use in a oxidase test?
Definition

N,N,N,N-tetramethyl-p-phenylenediamine

dihydrochloride

Term
what reagent would you use in a phenylalanine deamination
Definition
ferric chloride
Term

what reagent would you use in a

starch hydrolysis test

Definition
grams iodine
Term

what reagent would you use in a

tryptophan degradation test

Definition
Kovaks reagent
Term
what enzyme is used in casein hydrolysis
Definition
protease
Term
what enzyme is used in gelatin liquefication
Definition
protease
Term

what enzyme is used in

hydrogen sulfide production

Definition
cystein desulfurase
Term
what enzyme is used in indole production
Definition
tryptophanase
Term
what enzyme is used in starch hydrolysis?
Definition
amylase
Term
what enzyme is used in triglyceride hydrolysis?
Definition
lipase
Term
what enzyme is used in urea hydrolysis ?
Definition
urease
Term
what is the product of a catalase reaction?
Definition
molecular oxygen
Term
what is the product of a phenylalanine reaction
Definition
phenylpyruvic acid,and ammonia
Term

what is the product of a triglyceride

hydrolisis reaction?

Definition
fatty acids
Term

what id=s the product of a tryptophan

degredation reaction

Definition
indole and ammonia
Term

what is the product of an urea

hydrolysis reaction

Definition
ammonia
Term

what is the product of avoges-proskauer

test reaction

Definition
2,3-butanediol