Term
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Definition
Use (U): inoculate gel, cells that produce and exocytose amylase will digest starch (+); Gram's Iodine is used to bind starch, turning it brown
Results (R): (+) gap in brown coloring around colonies; (-) no gap |
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Term
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Definition
U: determine aerobicity of organism; liquid broths are inoculated, one is covered with oil
R: aerobes: (+) non-oil, (-) oil; anaerobes: (-)(+); facan: (+)(+); dead: (-)(-); acid: (+) green to yellow |
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Term
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Definition
U: scrape-inoculate across top of slant, if citrate is present, acid is created turning green agar blue (+)
R: (+) blue; (-) green |
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Term
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Definition
U: tests for denitrification (production of N2) as well as metabolism of urea
R: (+) gas in catcher, pink broth; (-) yellow broth, no gas |
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Term
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Definition
U: breakdown of esculin to esculetin, esculetin reacts with bile (brown)
R: (+) brown (+)not brown |
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Term
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Definition
U: determination of growth on a particular carbohydrate; growth produces acid, turns broth red
R: (+) red (-)not |
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Term
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Definition
U: determine degree of metabolism of carbohydrate
R: (+) high acidity, red (-) low-to-no acidity, not red |
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Term
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Definition
U: determine if glucose is digested to acetoin; acetoin reacts with alpha naphthol and KOH turns cherry red
R: (+) cherry red (-) yellow-brown |
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Term
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Definition
U: measures pH change due to breakdown of arginine, lysine, and/or ornithine
R: (+) purple/blue; (-) no color change |
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Term
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Definition
U: nitrate-->ammonia; nitrate broth, trap gas
R: (+) bubble (-) no bubble |
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Term
| Fluid Thioglycallate Media |
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Definition
U: determines aerobicity
R: Aerobe: all at surface; anaerobe: all away from surface; aerotolerant anaerobe: evenly distributed; facan: most at surface, but also elsewhere |
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Term
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Definition
eats up casein or doesn't
+: blank areas around colonies
-: continuous whiteness |
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Term
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Definition
eats gelatin or doesn't
+: becomes liquid
-: remains solid |
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Term
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Definition
breaks down DNA or doesn't
+: blue (breaks it down forms acid)
-: not (doesn't) |
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Term
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Definition
lipolysis capacity
+: clear patch around colonies
-: continuous blueness |
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Term
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Definition
| no test other than presence and growth (used for isolation) |
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Term
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Definition
H2S and indole production, motility
+++: black spots, red reaction to Kovac's reagent, spread from insertion
-++: no black spots
+-+: no reaction to Kovac's
++-: no spread |
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Term
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Definition
glucose/lactose fermentation; h2s production; gas production; inoculate slant and stab
++++: yellow butt, yellow slant, black butt, and crack/bubble
----: red butt, red slant, no black, no crack/bubble |
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Term
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Definition
blood agar, selective for gram positive, differential based on hemolysis
R: alpha: greenish; beta: turns gel bright yello; gamma: blueish |
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Term
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Definition
fermentation of lactose determinatoin by red pH indicator; selective for gram negative
R: +: red colonies -: colorless colonies |
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Term
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Definition
fermentation of lactose and/or sucrose dyes eosin Y & methylene blue; selective for gram negative
R: coliforms: blue-black; salmonella/shigella: colorless/amber; e coli: metallic green |
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Term
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Definition
U: lactose/sucrose/salicin fermentation (acid fuchsin/bromthymol blue); selective for gram negative
R: yellow/salmon: E. coli, entero, kleibs
blue-green: proteus, salmonella
green/moist: shigella
green-brown: pseudomonas |
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Term
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Definition
| Lactose fermentation determination; selective for gram negative rods |
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Term
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Definition
Determines if organism grows on mannitol/is halotolerant
R: +mann: yellow +growth: halotolerant |
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Term
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Definition
| selective for gram positive |
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Term
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Definition
if organism synthesizes and secretes catalase, administration of H2O2 will produce O2 gas
+: bubbles; -: no bubbles |
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Term
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Definition
testing presence of cyt. c oxidase (ETC) with sterile swab and a drop of tetramethyl-p-phenylenediamine
R: +: purple quickly -: no/slow color change |
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