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Details Title: lab test 2 Description: shs Total Flash Cards: 9 Created: 01/27/2005 20:12:05	To study from this set of flash cards, or to create your own your own study flash cards, register HERE. If you are already a registered user, CLICK HERE.
Cards Term chromatography Definition a way to separate pigments based on solubility in solvent, adhesion to chromatography paper, molecular size, and capillary action (cohesion and adhesion) Term chromatogram Definition chlorophyll rolled onto chromatography paper, chromatography placed in solvent, paper removed before solvent can reach top of paper, lighter and more soluble pigments travel most Term calculation for Rf Definition Rf = (distance pigment traveled) / (distance solvent traveled) Rf is an intensive property that can be used for identification Term rate of photosynthesis Definition spectrometer measures % transmittance of chlorophyll solution, DPIP used is dark blue when oxidized and, as it becomes reduced, becomes lighter and % transmittance will increase Term blank color Definition no DPIP and was used to set transmittance to 100% (control) Term dark / unboiled Definition DPIP was not reduced because no light was available to excite electrons from chlorophyll, there should be no change in the % transmittance Term light / unboiled Definition DPIP was reduced because the light was available to excite the electrons of the chlorophyll, there should be an increase in transmittance as the DPIP becomes reduced and the blue color becomes lighter Term light / boiled Definition DPIP is not reduced because the enzymes necessary for reduction are denatured, there should be no change int he transmittance Term no chlorophast Definition should show no change in transmittancee because chlorophyll was no available to supply electrons to reduuce the DPIP