Term
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Definition
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Term
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Definition
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Term
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Definition
| Eyepiece lens (usually 10x) |
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Term
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Definition
| Nosepiece lenses (commonly 4x 10x 45x 100x (oil immersion)) |
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Term
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Definition
| Mag(objective) x Mag(ocular) |
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Term
Important Parts of the Microscope:
-Framework |
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Definition
Arm, Base structural parts of the microscope which support the
basic frame. |
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Term
| Important parts of the microscope: Stage |
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Definition
Holds the slide. The mechanical stage clamps the slide and moves the
slide around the stage. |
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Term
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Definition
| Oculars, Objectives, and Condenser |
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Term
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Definition
| eyepiece lenses (usually 10x magnification |
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Term
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Definition
| lenses attached to rotatable nosepiece common magnifications of 4x, 10x, 45x (low power, high-dry objectives) and 100x (oil immersion lens). |
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Term
| __________ microscope focusing adjustments do not to be made when changing objective lenses |
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Definition
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Term
| Oil immersion lens uses oil with approximately the same refractive index as _____ to prevent light loss due to ________ (bending of light rays) which would occur if light traveled from one refractive index to another (eg. glass to air) |
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Definition
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Term
| As magnification of the objective lens increases, the working distance (distance between the object on slide and the objective lens, when in focus) _______ |
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Definition
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Term
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Definition
| directs light towards the objective lens in bright field microscopy (in dark field microscopy the condenser directs light at oblique angles away from the objective lens in a manner that allows only objects in the field of view to redirect or scatter light into the objective lens. This causes objects to appear white on a dark field). |
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Term
| Iris Diaphragm (lever located in the condenser) |
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Definition
| adjusts the diameter of the cone of light so that it just fills the objective lens |
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Term
•As you close down the diaphragm:
1. The light intensity _______
2. Contrast ________
3. Depth of field ________
4. _____ Resolution (with oil immersion lens) |
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Definition
1. decreases
2. improves
3. increases
4. Limit |
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Term
Resolution (_______ _____):
~ Expressed as d
~ d = the smallest distance between two objects which can be seen as separate
~ d = the diameter of the _____ _____ _____
d = λ / 2 NA
λ = wavelength of light
NA = numerical aperture
~ To improve resolution, lower d
d can be decreased by lowering _ or increasing the __ |
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Definition
| Resolving power ; smallest resolvable object ; λ ; NA |
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Term
Light Microscopy reveals three principle forms of microorganism:
1. More or less spherical organisms:
2. Cylindrical organisms:
3. Spiral shaped |
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Definition
1. COCCI
2. BACILLI
3. HELICOIDAL |
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Term
Incompletely separated cocci may appear in a number of different patterns depending upon the plane in which they divide and how they remain attached:
1. Diplococci (pairs) – divide in _ plane
2. Streptococci (chains) – divide in _ plane
3. Tetracocci (tetrads) – divide in _ planes
4. Staphylococci (clusters) – divide in ___ planes irregularly
5. Sarcinae (cuboidal packets) – divide in ____ planes regularly |
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Definition
1. one
2. one
3. two
4. three
5. three |
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Term
Bacilli can appear in a number of different cyclidrical shapes
1. _________ are very short and almost appears spherical, but they are just slightly longer in one direction then the other
2. ______ ______ are tapered at the ends, appearing as football like in shape
3. ________ ______ Forms grow in long threds |
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Definition
1. Coccobacillus
2. Fusiform Bacilli
3. Filamentous Bacillary |
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