Term
| The acronym that represents levels of precaution wen working with bacterial organisms is ____ |
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Definition
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Term
| Which objective lens(es) can be used to locate a specimen? |
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Definition
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Term
| Amoeba are found in what kingdom? |
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Definition
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Term
| A test which is poorly sensitive has too many ____ |
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Definition
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Term
| What type of hemolysis occurs when hemoglobin is completely broken down? |
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Definition
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Term
| When a MSA plate is inoculated, which organisms are selected and differentiated? |
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Definition
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Term
| An inoculated PEA plate will only grow |
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Definition
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Term
| What charge do basic stains have? |
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Definition
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Term
| What is the sugar metabolized by coliform bacteria? |
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Definition
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Term
| Acid-fast stain is used to primarily identify what genus? |
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Definition
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Term
| Which lens is known as the "high-dry" |
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Definition
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Term
| What is the purpose of the streak plate method? |
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Definition
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Term
| What is the name for general-purpose media? |
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Definition
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Definition
Use india ink/Nigrosin
Have a negative charge (repel stain) |
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Term
| Simple stains have a ____ charge |
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Definition
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Term
| What is another name for gram staining? |
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Definition
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Term
| what is another name for acid-fast stain? |
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Definition
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Term
| What is the primary stain for the Schaeffer-Fulton method? |
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Definition
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Term
| What is the counterstain in the endospore staining method |
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Definition
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Term
| What are the spore-producing genera? |
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Definition
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Term
| What is the pH indicator for MSA and what color does it turn when acid IS present? |
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Definition
Phenol red
yellow because it fermented the mannitol |
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Term
| Describe Phenylethyl Alcohol Agar (PEA) |
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Definition
Selective medium that allows growth of G+ org.s
Used to isolate Staph and strep |
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Term
| Describe Columbia CNA with 5% Sheep Blood Agar |
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Definition
Selective and differential
Allows growth of G+ bacteria
Colistin and nalidixic acid inhibit G- growth
Differentiation of G+ bacteria is based on their hemolytic reaction |
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Definition
"clear"
Complete hemolysis of RBCs |
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Definition
Greening of medium
Partially hemolyzes RBCs |
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Definition
Medium does not change color
Does not hemolyze RBCs |
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Term
| Describe Eosin Methylene Blue Agar |
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Definition
Sugars encourage growth of fecal coliforms
Sugar fermentation is depicted by a metallic green sheen(or growth is dark), pink coloration, or no change in color (nonfermenters)
Isolates fecal coliforms |
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Term
| Describe Hektoen Enteric Agar |
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Definition
Isolates Salmonella and shigella
Biosalts inhibit G+ cocci
yellow color=lactose fermentor
black precipitate=sulfer reducer
blue-green growth=no reduction or fermentation |
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Term
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Definition
Neutral red dye is a pH indicator
lactose fermentation turns dye red or pink
Selects G- bacteria
Crystal violet/bile inhibit G+ bacteria |
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Term
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Definition
"cold lovers"
Bacteria are either dormant or in suspended animation (not dead)
Group I:optimum temp is 15C
Group II: optimum temp 20-30C and contain spoilage microbes |
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Term
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Definition
Optimum temp 25-40C
Includes most disease-causing organisms
Like room to body temperature |
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Definition
Optimum growth 50-60C
Not considered a public health problem |
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Term
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Definition
| Bacteria that are tolerant of acid pH |
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Term
| Define Obligate Acidophile |
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Definition
| Bacteria that produce acids |
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Term
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Definition
| Bacteria that like pH greater than 7.5 |
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Definition
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Definition
Aerobic
Grow only in Oxygen concentration less than air
Optimum oxygen concentration is 1%-15% |
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Term
| What is used to detect the presence of starch in starch hydrolysis? |
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Definition
Iodine
It reacts with starch to produce a blue or dark brown color |
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Term
| When looking at a petri dish, the medium is black with a clearing around the organism. What enzyme is present? |
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Definition
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Term
| What gives milk its white color? |
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Definition
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Term
| What agar is used in a Lipid Hydrolysis test? |
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