First, pick the properly sized pipet. Then, put a properly sized tip on by lightly tapping the pipet into the tip. Adjust the volume the pipet will uptake. Depending on the pipet model, you will either have to:

• Push down the button on the side of the pipet and twist the knob until the interface reads 10 microliters
• Pull up on the knob of the pipet and twist it until the interface reads 10 microliters

Push the knob down to one click and stick the pipet into the volume you are uptaking. Place the tip of the pipet in the tube you are measing your volume in and push the knob down to two clicks to ensure that all of the liquid comes out. Tap the inner side of the tube with the tip to get the last drop out.

Push the pipet down to three clicks to release the tip.

1. Loosen the head and twist around, re-tighten.
2. Plug microscope in.
3. Place the sample on the specimen stage.
4. Move the condensor ~1 mm below the stage.
5. Put the specimen in place using the x and y coordinates.
6. Rotate the 10x magnification into place using the grip.
7. Adjust the eye width. Focus using the coarse focus.
8. Rotate the 40x mag. in to place using the grip. Use the coarse focus to focus the specimen. Close the left eye and use fine focus. Close the right eye and use the diapter focus. 
9. Adjust the diaphram to optimal light.

How would you make a V ml solution with a C dillution ratio?

How would you analyze its wavelength using the spectrophotometer?

To make the solution, use C₁V₁ = C₂V₂, where on the exam C₁ = 1.0 M, V₁ is the volume of solute being solved for, C₂ is the desired concentration and V₂ is the desired volume.

To use a spectrophotometer, begin by turning it on. Set the wavelength to the correct number using the dial on top of the machine and make sure the wavelength lever below is set to the correct incriment. Without any vials in the machine, use the left knob to set the transmittance to 0. Place a blank in the machine and use the right knob to set the transmittance to 100%. Clean the vial being tested with a Kimwipe and place in the machine to test for absorbance.

How would you measure something using a vernier caliper?

How do you place tubes correctly in a centrifuge?

On the caliper, only play attention to the cm scale on the bottom half of the caliper, along with its minor scale underneath it. 

Insert the object being measured in the jaw. Where the "0" mark on the minor scale lies in relation to the major scale is the first number to the right of the decimal point. For example, if the 0 on the minor scale lies between the 4.7 and 4.8 on the major scale, the first number to the right of the decimal point for the object's length is 7.

Then look for which dash on the minor scale lines up with a line on the major scale. Take this measurement and multiply it by what each increment represents (given, ussually 0.05 mm). Say the 7 dash on the minor scale lines up with a line on the major scale. If there is one dash between each number on the minor scale, that means there are 14 increments and they each represent 0.05 mm. 14 * 0.05 mm = 0.70 mm = 0.07 cm. So the second number is a 7 as well. The object's length is ~0.77 cm.

To balance a centrifuge make sure all vials are spread out evenly. Break groups of 3 into triangles and groups of 2 into lines.