Term
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Definition
the ability to split disaccharides and use glucose to produce pyruvate for fermentation
glucose (monosaccharide), lactose (glucose&galactose), sucrose (glucose&fructose) |
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Term
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Definition
fermentation w/ acid and gas products
A/G |
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Term
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Definition
fermentation w/ acid production, no gas production
A/- |
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Term
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Definition
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Term
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Definition
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Term
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Definition
degradation of peptone, alkaline end product
K |
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Term
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Definition
enzyme that breaks down highly reactive (toxic) hydrogen peroxide
Gram positive bacteria |
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Term
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Definition
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Term
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Definition
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Term
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Definition
| determines if a bacterium possesses nitrate reductase enzyme, which breaks nitrate down to nitirite |
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Term
| Gas (known non-fermenter) |
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Definition
dentrification (production of nitrogen gas)
G |
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Term
| Red (after adding reagent A&B) |
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Definition
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Term
| No color change (after adding zinc) |
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Definition
nitrate reduction to non-gaseous nitrogenous compounds
+2 |
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Term
| Red (after addition of zinc) |
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Definition
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Term
Citrate
-sodium citrate
-ammonium ion |
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Definition
Determines if bacterium can use citrate as sole carbon source
non-motile Enterobacteriaceae |
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Term
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Definition
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Term
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Definition
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Term
| No color change, no growth |
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Definition
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Term
Decarboxylation
lysine
ornithine
arginine |
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Definition
enzymes that remove carboxyl group (COOH) from amino acids
pH indicator brom cresol purple
Enterobacteriaceae |
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Term
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Definition
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Term
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Definition
Fermentation, no decarboxylation
- |
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Term
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Definition
decarboxylation, organism produces specific enzyme
+ |
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Term
Urease
phenol red is pH indicator |
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Definition
enzyme that hydrolyzes C-N bonds of urea (urease)
requires water, products are CO2 and NH3
the ammonia increases pH
Proteus? |
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Term
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Definition
rapid urea hydrolysis, strong urease production
+ |
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Term
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Definition
slow urea hydrolysis, weak urease production
weak + |
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Term
| orange/yellow --> partially pink |
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Definition
slow urea hydrolysis, weak urease produciton
weak + |
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Term
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Definition
no urea hydrolysis, urease is absent
- |
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Term
SIM medium
Kovac's reagent (hydrochloric acid, 1-butanol, dimethylaminobenzaldehyde) |
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Definition
Sulfur Reduction
Indole Production
Motility
differentiate between Salmonella and Shigella |
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Term
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Definition
sulfur reduction
H2S produced
+ |
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Term
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Definition
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Term
| Red in alcohol layer of Kovac's reagent |
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Definition
tryptophan broken down into indole and pyruvic acid
+ |
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Term
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Definition
tryptophan not broken down
- |
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Term
| Growth radiating from stab line |
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Definition
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Term
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Definition
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Term
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Definition
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Term
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Definition
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Term
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Definition
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Term
Triple Sugar Iron Agar
useful w/ MacConkey, EMB and SS agar |
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Definition
tests for fermentation, gas production and sulfur reaction from gram negative enterics
dextrose (glucose), lactose, sucrose, sodium thiosulfate, phenol red |
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Term
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Definition
glucose and lactose and/or sucrose fermentation w/ acid accumulation in slant and butt
A/A |
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Term
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Definition
glucose fermentation w/ acid production; peptone catabolized aerobically(in slant) w/ alkaline products (reversion)
K/A |
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Term
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Definition
No fermentaiton; peptone catabolized aerobically & anaerobically w/ alkaline products
K/K |
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Term
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Definition
exotoxins produced by some species of gram positive cocci
destroy RBCs and Hb
use to obtain nutrients from host cells (iron can be limiting factor) |
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Term
| red slant/no change in butt |
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Definition
no fermentation; peptone catabolized aerobically w/ alkaline products
K/NC |
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Term
| no change in slant/no change in butt |
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Definition
organism is growing slowly or not at all
NC/NC |
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Term
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Definition
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Term
| Cracks in or lifting of agar |
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Definition
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Term
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Definition
cultivate fastidious microorganisms, detect hemolytic ability,
5% sheep blood
differentiate species of Streptococcus and Staphylococcus |
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Term
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Definition
organism hemolyzes RBCs completely
beta hemolysis |
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Term
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Definition
hemolyzes RBCs partially
alpha hemolysis |
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Term
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Definition
does not hemolyze RBCs
gamma hemolysis |
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