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Lab Final Definitions
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53
Microbiology
Undergraduate 2
04/25/2011

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Term
Mixed culture
Definition
a microbial culture consisting of two or more species.
Term
Pure culture
Definition
a microbial culture consisting of only a single species.
Term
Media
Definition
Solid or liquid substances that are used for maintenance or cultivation of bacterial and fungal species.
Term
Selective Media
Definition
a type of media that is designed to enhance the isolation procedure by inhibiting the growth of some organisms while encouraging the growth of other organisms.
Term
Differential Media
Definition
a type of selective media that contains indicators that expose the differences between organisms.
Term
Undefined/Complex Media
Definition
media that are composed of extracts from plant or animal sources and are rich in nutrients. It contains one or more ingredient made up of known ingredients, but of unknown composition, such as yeast extract, beef extract, or digest of gelatin. It supports the growth of a greater variety of microbes.
Term
Defined Media
Definition
media in which the amount and identity of every ingredient is known. It supports a narrower range of organisms.
Term
Coliforms
Definition
a subgroup of Enterobacteriaceae that produce gas from lactose fermentation. Most coliforms are inhabitants of the human intestinal tract and are isolated in fecal samples. Escherichia coli is the most common coliform.
Term
Nutritional Component (in selective media)
Definition
= substances that are selected to obtain the optimum growth of an organism that is being tested or that is suspected of being in a sample.
Term
Inhibitors (in selective media)
Definition
substances that make the media selective and are designed to exploit the weaknesses in specific groups of organisms and thus prevent or inhibit their growth, while allowing other organisms to grow.
Term
Indidcators (in selective media)
Definition
substances that make a desired or expected reaction visible. The indicator is usually a dye that changes color as the pH changes. An indicator can also be a chemical that reacts with the products of a reaction to produce a color change.
Term
Family Enterobacteriaceae
Definition
enteric “gut” bacteria
Term
Mixed acid fermentation
Definition
is an anaerobic fermentation where the products are a complex mixture of acids, particularly lactate, acetate, succinate and formate as well as ethanol and equal amounts of hydrogen gas and carbon dioxide.
Term
2,3-butanediol fermentation
Definition
anaerobic fermentation of glucose with 2,3-butanediol as one of the end products. The other end-product is acetoin.
Term
acetoin
Definition
s an end-product of glucose fermentation that is used as an external energy source by many fermentive bacteria. It is, also, a common precursor in the biosynthesis of branched chain amino acids.
Term
superoxide dismutase
Definition
is an enzyme that catalyzes the conversion of superoxide radicals (O2-) to hydrogen peroxide.
Term
catalase
Definition
is an enzyme that converts hydrogen peroxide to water and gaseous oxygen.
Term
cytochromes
Definition
are membrane-bound hemoproteins that contain heme groups and carry out electron transport.
Term
cytochrome c
Definition
is a component of the electron transport chain in mitochondria.
Term
cytochrome c oxidase
Definition
is an enzyme that can oxidize cytochrome c and catalyze the reduction of cytochrome c by a chromogenic reducing agent.
Term
chromogenic reducing agent
Definition
is a chemical that develops color as it becomes oxidized.
Term
nitrate reductase
Definition
is an enzyme that reduces nitrate to nitrite in
Term
Denitrification
Definition
is a process of nitrate reduction that may ultimately produce molecular nitrogen (N2) through a series of intermediate gaseous nitrogen oxide products.
Term
citrate-permease
Definition
is an enzyme that can transport citrate molecules into the cell and enzymatically covert the molecules into pyruvate if citrate is the only carbon source available.
Term
-amylase (alpha)
Definition
is an enzyme capable of hydrolyzing starch by breaking the glycosidic linkages between the sugar subunits.
Term
oligo-1,6-glucosidase
Definition
is an enzyme capable of hydrolyzing starch by breaking the glycosidic linkages between the sugar subunits.
Term
Urease
Definition
is an enzyme that can hydrolyze urea to form ammonia and carbon dioxide.
Term
Casease
Definition
an enzyme capable of hydrolyzing the milk protein casein.
Term
Casein
Definition
is a milk protein that gives milk its white color.
Term
Gelatinases
Definition
is an extracellular enzyme that is capable of hydrolyzing gelatin.
Term
Deoxyribonuclease (DNase)
Definition
is an enzyme that is capable of catalyzing the depolymerization of DNA into small fragments.
Term
Cysteine disulfurase
Definition
is an enzyme that functions in the reduction of sulfur to hydrogen sulfide (H2S) by catalyzing the putrefaction of the amino acid cysteine to pyruvate.
Term
Thiosulfate deductase
Definition
= is an enzyme that functions in the reduction of sulfur to hydrogen sulfide (H2S) by catalyzing the reduction of sulfur in the form of sulfate at the end of the anaerobic respiratory electron transport chain.
Term
Tryptophanase
Definition
is an enzyme that can hydrolyze tryptophan to pyruvate, ammonia by deamination, and indole.
Term
Germ Theory of Disease
Definition
is a theory that proposes that microorganisms are the cause of many diseases.
Term
Disinfectant
Definition
is a chemical germicide that is designed for use on liquids and surfaces such as floors, tables, sinks, countertops, and surgical instruments.
Term
Antiseptic
Definition
is a chemical germicide designed for use on or in living tissue.
Term
Use-Dilution Test
Definition
is a standardized procedure used to measure the effectiveness of disinfectants specifically against Staphylococcus aureus, Salmonella enterica, and Pseudomonas aeruginosa
Term
Epidemiology
Definition
is the study of the causes, occurrence, transmission, distribution, and prevention of diseases in a population.
Term
Morbidity
Definition
sickness
Term
Mortality
Definition
death
Term
Infectious Diseases
Definition
are diseases caused by biological agents such as bacteria and viruses.
Term
Noninfectious diseases
Definition
are diseases not caused by biological agents and include stroke, heart disease, and emphysema.
Term
Fomites
Definition
contaminated objects
Term
Common Source Epidemic
Definition
is when a disease is transmitted from an area that is capable of infecting many people at once. Example: an entire community sickened due to a contaminated water supply.
Term
Propagated Transmission
Definition
is when a disease is transmitted from person to person.
Term
Index Case
Definition
is the first case of a disease spread by propagated transmission.
Term
Hemolysins
Definition
exotoxins that are able to destroy red blood cells and hemoglobin.
Term
Antibiotics
Definition
are natural antimicrobial agents produced by microorganisms.
Term
antimicrobials/antimicrobics
Definition
are substances that are used to treat bacterial infections.
Term
Zone of Inhibition
Definition
the clear zone around an antimicrobial disk where the growth of the bacterial organism has been inhibited.
Term
Bactericidal
Definition
a drug that kills bacterial organisms
Term
Bacteriostatic
Definition
is a drug that can stop the growth of bacterial organisms but do not kill the organisms.
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