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Lab 5
Review
13
Chemistry
Undergraduate 3
02/13/2012

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Cards

Term
Substrate for acid phosphatase in this experiment
Definition
p-nitrophenyl phosphate disodium salt
Term
How do we determine unknown concentrations?
Definition
using a standard curve
Term
Michaelis constant
Definition
Km
Term
Enzyme of prostate cancer
Definition
Prostatic acid phosphatase
Term
Function of Restriction digestion enzymes
Definition
Cut DNA into fragments
Term
Enzymes for drug metabolism
Definition
P450 enzymes
Term
As more enzyme binds to substrate, what happens to the velocity of product formation?
Definition
It increases
Term
Initial Velocity
Definition
When increase in
substrate shows a proportional
increase in rate of formation of product
Term
Maximum Velocity
Definition
When increase in substrate shows no increase in rate of formation of product. The velocity at maximal concentrations of substrate
Term
Michealis Menten constant
Definition
concentration of substrate that gives "half-maximal velocity"
Term
Which organ has an unusually high concentration of acid phosphatase?
Definition
adult prostate gland
Term
For this lab, where does the Acid Phosphatase come from?
Definition
A potato
Term
Where do we dispose cuvettes
Definition
in regular garbage
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