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lab 2
micro lab quiz 2
26
Microbiology
Undergraduate 1
08/08/2013

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Term
Chemically defined media
Definition
A medium in which the exact chemical composition is known.
Term
Complex media
Definition
Media for which the exact chemical compostion varies slightly from batch to batch.
Term
3. Nutrient broth
Definition
commonly used liquid complex medium.
Term
4. Nutrient agar
Definition
agar added to nutrient broth making it a solid medium.
Term
5. Agar
Definition
A polysaccharide extract from red marine algae that is used as solidifying agent for culture media.
Term
6. Agar liquefies at
Definition
100 degrees Celcius
Term
7. Agar solidifies at
Definition
40 degrees Celcius.
Term
8. Steam sterilization
Definition
Common method for sterilizing culture media that are heat stable.
Term
9. Autoclaving
Definition
using steam under pressure. 121 degrees Celcius at 15psi for 15 minutes.
Term
10. Petri plates
Definition
containing solid media provide a large surface area for examination of colonies. Leave lid ajar so no condensation and let heat escape, help solidify faster.
Term
11. Inoculated
Definition
microbes intentionally introduced.
Term
12. Incubation period
Definition
The bacteria that are inoculated into culture media increase in number.
Term
13. Colony
Definition
Population of cells that arise from a single cell.
Term
14. Turbid
Definition
cloudy
Term
15. Colony forming unit
Definition
A colony that arises from a group of the same microbes attached to one another
Term
16. Flocculent
Definition
clumps of microbial cells
Term
pellicle
Definition
membranes
Term
Sediment
Definition
microbial cells that have settled to the bottom
Term
inoculated
Definition
introduced into various forms of culture media
Term
contaminates
Definition
unwanted microbes
Term
Aseptic technique
Definition
Used in microbiology to exclude contaminants
Term
Sterilized
Definition
Rendered free of all life
Term
Agar slants
Definition
– Test tubes containing solid culture media that were left at an angle while the agar solidified. (Easier to store and transport)
Term
agar deep
Definition
Agar is allowed to solidify at the bottom of a test tube. Can be stored for a long period.
Term
inoculating loop
Definition
heat-resistant, non-corroding Nichrome wire that is bent into a loop
Term
inoculating needle
Definition
Sterile, heat-resistant, non-corroding Nichrome wire that is straight at the end.
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