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L1: Aseptic techinque
asepsis, patient prep., surgeon's prep., surgical instruments, prophylatic ABs
68
Veterinary Medicine
Undergraduate 3
06/02/2012

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Term
what should any surgeon consider when treating any illness?
Definition
relative risks and rewards
Term
surgical risk assessment
Definition
consider...
-presence of any primary disorder or other illness
-potential for complications
-patient's use and quality of life
-long term prognosis
Term
what incr. surgical risk?
Definition
-emergency procedures
-longer surgical times
-less experienced surgeons
Term
how can surgical risk be decreased?
Definition
ASEPTIC TECHNIQUE
minimising tissue trauma by using good and min. invasive procedures where possible
-knowledge of the procedure
-avoiding unnecessary delays
-appropriate closure
-good post-op care
-monitoring
Term
what are post operatative surgical site infections (SSI) a major cause of?
Definition
morbidity and even mortality
Term
what incr. the risk of surgical site infection (SSI) so that additional measures (e.g. prophylatic antimicrobials) are needed to decr. risks?
Definition
-microbe related factors (interplay amoung the no. of inf. organisms, their virulence, and hosts local and systemic dz)
-host related factors (e.g. systemic factors and decr. im resp., remote trauma or inf., cell damage by toxins, presence of FB)
-surgery related factors (emergency, longer duration, surgeon experience and technique,surgical implants e.g. sutures)
Term
general intra-op recommendations to decr the risk of SII?
Definition
-use disposable water-R gowns and drapes
-change gloves that are punctured or torn
-minimise tissue trauma
-maintain good haemostasis
-irrigate wounds during surgery
-debride traumatic wounds thoroughly
-minimise sutures
-avoid dead spaces
-close in layers w/o tension
-keep surgery time to a minimum
Term
what is the primary source of pathogens in surgery?
Definition
the patient - any concurrent illnesses or dz. should ideally be tx. before surgery
Term
asepsis
Definition
the process of removing pathogenic micro-organisms or protecting against these organisms
Term
how can asepsis be achieved?
Definition
systematically eliminating sources of contamination
i.e. op theatre, surgeon, instruments
Term
operating theatre asepsis
Definition
-traffic through the theatre should be minimal and one-way
-any person entering should wear appropriate surgical attire
-should be cleaned and disinfected regularly
-adequate drainage is important
-only essential furniture should be present
-air should be under mild positive P - air flows out rather than in
Term
if there is only one theatre available. how should asepstic technique be maximised?
Definition
-clean surgeries e.g. fractures should be performed before contaminated surgeries e.g. purulent abscess
Term
patient prep
Definition
skin prep:
- hair removal
-cover feet
-clean skin to remove gross contamination
-disinfect skin
Draping patient
Term
hair removal of patient
Definition
-clipping not shaving (causes abrasions and so predisposes to bact. colonisation)
-preferably outside theatre and before anaes. induction
-should be at least 10-15cm away from intended surgical site
Term
cleaning skin to remove gross contamination
Definition
-apply scrub solution w. gloved hands
-use sufficient water
-scrub until clean +/- 5 mins
-rinse inbetween
-whipe a white swab over area at end - should be clean
Term
disinfection of skin
Definition
-used gloved hands and swabs w. scrub solution and water
-start at surgical incision and work out
-5 mins
Term
Antiseptics
Definition
-chlorhexidine
-Iodine compounds
-alcohol
Term
Chlorhexidine
Definition
-disinfectant
-residual activity (binds to protein in skin) ~4-5hrs - so longer on skin, more protection i.e. time dependent activity
-low toxicity - bit can be toxic for fibroblasts
Term
Iodine compounds
Definition
-disinfectants
-no residual activity
-only free iodine is bactericidal i.e. iodine scrub must be diluted or combined w. detergent to release free iodine
-low toxcity
-synergistic w. alcohol
but...stains, radiopaque, smells
-inactivated by inorganic debris - hence need good gross decontamination!
Term
Alcohol
Definition
-disinfectent
-only active against bacteria
-no residual activity
-inactivated by inorganic debris
-commonly used as a rinse after surgical scrub i.e. remove soap / antiseptic etc
Term
Draping patient - goal
Definition
prevent mvmt of debris and bacteria from non-sterile areas to surgical site
Term
drape material
Definition
-impermeable, disposable drapes are best
-cloth drapes become permeable to bact. once wet
Term
drape technique
Definition
-drape from clean to dirty
i.e. do not slide surgical drape over patient towards surgical site, but from site outwards
-surgeon keep hands on sterile parts of drapes - double gloves recommended (then remove second gloves before incision)
Term
use of additional drapes
Definition
-can be used during surgery if concerned about contamination
Term
position of drapes
Definition
-held in place by using towel clamps
-remember, if these are removed during the procedure, they are not sterile!
Term
surgeon's prep.
Definition
-designated clothing
-hand disinfection / scrubbing
-gowning ang gloving
Term
designated theatre personnel clothing
Definition
-scrubs - shirt tuked into trousers
-cap (avoid hair contamination)
-mask
-designated footwear - only to be worn in theatre (or foot covers)
Term
hand disinfection (scrubbing) - aim
Definition
-remove gross contamination
-decr. bact. count on skin
-ideally prolonged effect on transient and resident microflora should be achieved (contaminents from environ. and permanent)
Term
why is scubbing so important?
Definition
-accepted industry standard is that 1.5% of gloves are punctured before use
-at end of surgery 30% of gloves are punctured
Term
hand disinfection - nails
Definition
nails should be kept short, clean and free of polish and artificial nails
Term
Hand disinfection - scurb protocol
Definition
-wash hands and forearms w. water and soap
-clean fingernails
-use surgical scrub brush to wash w. antiseptic soap - repeat routine twice, w. rinsing between
-total scrub time 3-5 mins
-dry w. sterile towels
Term
what is important to remember when applying surgical scrub?
Definition
-contact time is important
-make sure include front and back of hands, four sides of each finger and thumb, fingertips, fingernails, between each finger, wrists and forearms
-keep hands above elbows at all times
Term
what it is important to remember when drying hands post scrubbing?
Definition
-use sterile towels!
-hands first, then wrist to elbow and discard towel
-second towel for other hand and forearm
Term
antiseptic soaps
Definition
-povidone iodine
-chlorhexidine gluconate - superior due to prolonged residual activity
Term
main principle when applying gloves
Definition
fingers do not leave gown until covered w. glove to decr. contamination
Term
gowning and gloving - what must be remembered when putting on
Definition
-put on w/o ever touching the outside surface!
Term
gowns material
Definition
water resistent
comfortable
breathable
Term
what is preferable technique for applying gloves?
Definition
closed gloving
Term
aseptic technique involving surgical instruments
Definition
-cleaning
-packing
-sterilsation
Term
cleaning of surgical instruments
Definition
-should be rinsed immediately after use to remove blood and debris
-complex instruments should be disassembled
-after cleaning, instruments should be placed in instrument milk / oil to lubricate and protect surface
Term
cleaning methods of instruments
Definition
-manual e.g. warm soapy water or enzymatic detergent
-ultrasonic cleaner - uses high-freq vibratory waves that clean through caviation
Term
packing of clean surgical instruments
Definition
-marked w. content, date of sterilisation, person responsible
-crepe papers > cotton/ muslin wraps (more durable, good handling qualities and longer storage times)
Term
sterilisation methods
Definition
-heat
-chemical - ethylene oxide
-gas plasma
-peracetic acid
-electrolysed acid water
Term
most common and practical sterilisation method?
Definition
heat sterilisation
Term
moist heat sterilisation
Definition
moist heat kills by coagulation of critical proteins
Term
dry heat sterilisation
Definition
kills by oxidation and removal of water
Term
moist or dry heat sterilisation?
Definition
moist > dry
because can decr. temp and exposure times necessary for sterilisation
Term
Min. guidelines in steam autoclave for sterilisation
Definition
15min - 121C - 2atm
Term
Steam autoclaves
Definition
-air displacemtn by steam, either by gravity or creating a vacuum
-air displacment by steam is critical to achieve condensation which is necessary to transfer heat to the instruments
Term
placement of instruments in autoclave
Definition
so air does not get trapped and no air pockets persist - ensure enough room between packs
e.g. place bowls w. opening facing down (NOT as if using)
Term
Bowie-Dick test
Definition
-commercially available test to ensure autoclave functioning well - should be tested regularly
-ensures that air removal and stram penetration are complete
Term
why use indicator systems in autoclaves?
Definition
-used to ensure that instruments are exposed to adequate times and temperatures
Term
types of indicator systems in autoclaves
Definition
-chemical indicators change colour on exposure to sterilising temp
-biological indicators have heat-R bact spores that require a period of incubation after sterilisation to ensure absence of bact. growth - not as pratical
-indicator tape on outside of pack - inferior! colour change on outside does not mean contents are sterile!
Term
most commonly used agent in chemical sterilisation
Definition
ethylene oxide
Term
ethylene oxide chemical sterilisation
Definition
-alkylating agent - kills by inactivation of proteins, DNA and RNA
-gas so rapidly penetrates packaging and items at low temperature
-tolerated by almost all materials
Term
when should ethylene oxide chemical sterilisation be used?
Definition
-should only be used for items that are not suitable for heat sterilisation
Term
limitations of ethylene oxide
Definition
-size of equipment
-time requirement (often requires 24hours!)
-concerns about toxicity (for handler and animal)
Term
gas plasma chemical sterilisation
Definition
-H2O2 is injected into chamber -radiowaves create an electrical field and so H2O2 converted to gas plasma and free radicals that ollide w. and inactivate microorganisms
Term
peracetic acid chemical sterilisation
Definition
aka cold sterilisation - can be used at room temp
-it inactivates proteins
-contact time 10-15min
-0.09% conc recommended
Term
Electrolysed acid water sterilisation
Definition
generates hydroxy free radicals w. rapid and potent bactericidal effect
Term
when should prophylatic antimicrobials be used?
Definition
only in procedures w. high liklihood of infection
e.g. using implants / when development of inf. is life threatening
-any procedure in which the wound is anything but "clean"
Term
clean wound
Definition
-elective procedure
-aseptic technique has been used
-closed primarily
-no drain placed
Term
clean-contaminated wound
Definition
-as for clean wound but during the procedure...
-the GI, resp or urogenital tract has been entered under controlled conditions and w. usual contamination
+/- there was a minor break in aseptic technique
Term
contaminated wounds
Definition
-traumatic wounds
-gross spillage of GIT
-presence of an acute non-purulent inflammation
-major break in aseptic technique
Term
Dirty and infected wounds
Definition
-if exudates or perforated viscous is encountered during the procedure
-traumatic wound w. delayed tx, devitalised tissue, faecal contamination +/or FBs
Term
which prophylatic antimicrobials?
Definition
-should always be bactericidal
-not the same as antimicrobials used to tx. established inf.
-need knowledge of most common bact. isolated from SSI in specific procedures e.g. only skin contamination then gram +ve spectrum is probably adequate
-also consider cost, admin, potential s. effects
Term
how long use prophylatic antibodies?
Definition
- no apparent benefit to continuing antimicrobial prophylaxis for more than 24hours
-bact. R can be promoted gr. than this
Term
if bact. inf. occurs despite prophylatic antimicrobials...
Definition
...bact should be considered R and therapeutic antibodies should be given (preferably based on culture and susceptibility)
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