Term
| Gel Electrophoresis Definition |
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Definition
| separation of charged molecules by difference in their rate of migration in an electric field |
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Term
| Gel Electrophoresis Samples Used |
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Definition
serum
RBC hemolysates
urine, if concentrated x100
cerebral spinal fluid |
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Term
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Definition
monoclonal: one protein present
polyclonal: multiple proteins present |
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Term
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Definition
| the pH at which a protein is neutral |
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Term
| pH variables versus isoelectric setpoint |
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Definition
if pH is higher, protein carries negative charge and migrates towards positive anode
if pH is lower, protein carries positive charge and migrates towards the cathode |
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Term
| Rate of Migration Definition |
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Definition
depends on the ner charge, size, and shape of the molecule
strength of the electric field, the prosity, viscosity, and temp of the support media |
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Term
| Electrophorsesis Components |
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Definition
proteins and nucleic acids (to be separated)
support medium: gel (strach, polyacrylamide or agarose)
buffer system
DC power source: 50 - 1000 volts |
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Term
| Support Media for Biological Molecules |
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Definition
proteins: native gel (acrylamide or starch) or denaturing gel (acrylamide)
nucleic acids: agarose gel or acrylamide gel |
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Term
| Chromotography Definition |
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Definition
| separation of a mixture based on deifferences in the solutes' attraction for a moving/mobile phase and its attraction for the stationary/solid phse |
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Term
| Stationary Phase Definition |
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Definition
solid or solid coated with a liquid which remains in a fixed position
paper, alumina gel, cellulose acetate, polymers |
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Term
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Definition
liquid or gas which moves through the stationary phase carrying the mixture through the system
helium gas, organic solvents, buffers, ionic solutions |
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Term
| Migration Rate Definition |
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Definition
| interation fo the physical properties of the mixture between the mobile and stationary phase |
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Term
| Retention Time Definition |
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Definition
| time it takes a substance to migrate to an endpoint or be eluted from a column |
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Term
| Steric/Size Exclusion/Gel Permeation Definition |
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Definition
porous particles packed inside a columb
solution is poured into the column, large particles come out first |
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Term
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Definition
separation based on charge
cation exchange: elutes anion first
anion exchange: elutes cation first |
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Term
| Thermal Conductivity Definition |
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Definition
| two filaments are exposted to carrier gas alone or two filaments are exposted to gas plus sample, the change in temperature results in change in resistance |
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Term
| Hydrogen Flame Ionization Definition |
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Definition
| organic compounds burned in the flame produce electrons that are attracted to the anode causing a current flow |
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Term
| Electron Capture Definition |
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Definition
| beta emitters ionize the carrier gas, the standing current develops across the opening and any halgentaed compounds in the sample will capture the standing electrons causing a change in current |
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Term
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Definition
law stating that current is directly proportional to voltage and is inversely proportional to resistance
equation: Current = Voltage/Resistance |
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Term
| Equation to Solve for Voltage |
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Definition
| Voltage = Current x Resistance |
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Term
| Equation to Solve for Resistance |
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Definition
| Resistance = Volage/Current |
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