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Inoculation
Agar Plates and what grows
13
Biology
Undergraduate 1
12/07/2007

Additional Biology Flashcards

 


 

Cards

Term
Throat cultures set up on what plates?
Definition
Blood Agar only
Term
Lower Respiratory cultures are set up on what media.
Definition
BAP, Chocolate, and Mac.
Term
Transtrachial aspirate specimens are set up on what media.
Definition
BAP, Chocolate, Mac, and Thio
Term
Cystic Fibrosis Patients add what media?
Definition
PC Agar
Term

Sheep Blood Agar (SBA/BAP)

Definition

Trypticase soy agar with 5% sheep blood.

 

  • Differential (based on hemolysis)
  • Enriched (blood)
Term

MacConkey Agar

Definition

Agar with peptone base containing lactose.

  • Selective (GNR, bile salts)
  • Differential (Lactose fermenting)
Term
Chocolate Agar
Definition

Peptone agar spplemented with hemolyzed, defibrinated blood.

Isolation of Haemophilus & Pathogenic Neisseria.

Enriched (everything likes chocolate)

Term
CNA Agar
Definition

Columbia agar base with colistin and nalidixic acid

Enhibit growth of GN organisms.

Selective for GP organisms

Term
GC Lect Agar
Definition

Essentially the same as chocolate agar w/ antibiotics.

  • Enriched
  • Selective (Neisseria Gonorrhoeae)
Term
Hektone Agar
Definition

Peptone base agar with lactose, sucrose, salicin and ferric ammonium citrate and bile salts.

Differential (H2S producing/blue-green vs yellow)

Selective (Gram Negative Organisms)

Term
Campy Blood Agar
Definition

Brucella agar base w/ 10% sheep blood. 

  • Selective (Numerous antibiotics) Campylobacter
  • Enriched (Whole blood)
Term
SMAC (Sorbitol MacConkey)
Definition

MacConkey agar with sugar D-sorbitol

  • Enriched
  • Selective
  • Differential (E coli 157:H7)
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