Term
| Structure of B cell receptor |
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Definition
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Term
| What cells would find immunoglobulin domains on? |
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Definition
B cell receptors T cell receptors MHC moleculres
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Term
| How would you make monovalent Fab fragments in the lab? how would you make bivalent fragments? |
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Definition
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Term
| Where in the AA chain is the variable region found in the Fab segment? Fc segment? |
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Definition
at the N terminal end at the C terminal end |
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Term
| Describe what hypervariable regions are |
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Definition
| certain complementary determining regions (CDR's) that give receptor its specificity (where it binds Ag) |
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Term
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Definition
| part of antigen that binds to immune receptors |
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Term
| How does an antibody know what antigen to bind |
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Definition
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Term
| Difference between immunogen and antigen |
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Definition
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Term
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Definition
antigens that bind to immune receptor and do not cause an immune response |
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Term
| How can one elicit an immune response to a hapten? |
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Definition
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Term
| clinical applications where haptens are used |
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Definition
vaccines (ex: flu, pneumococcal) diagnosis Penicllin allergy (binds to hapten at beta lactam group to cause immune response)
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Term
| What determines isotype of B cell receptor/antibody? |
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Definition
| constant region of Ig heavy chain (associated with different functions) |
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Term
| What types of epitopes do B cell receptors tend to recognize? |
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Definition
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Term
Epitopes that tend to be recognized by T cell receptors and how they must be presented? |
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Definition
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Term
| Structure of T cell receptor |
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Definition
heterodimers of alpha, beta chains variable and constant regions as well can have gamma-delta type (primative)
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Term
| What is ment by MHC restriction? |
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Definition
| T cells will recognize peptide on APC ONLY if they recognize the MHC and physically bind. |
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Term
| Describe how superantigens work |
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Definition
THE EXCEPTION TO T CELL RULES |
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Term
How do we generate antigen receptor diversity when we do not have enough gens to make millions of receptor types for B and T cells? |
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Definition
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Term
| What site specific mini gene segments are targeted for recombination? |
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Definition
variable region (V) diversity region (D) joining region (J)
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Term
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Definition
RAG bind to RSS to initate gene rearrangement bring together D and J segment DNA cleaved to create hairpin structure at end of segment other proteins bind to hairpin hairpins cleaved TdT adds more nucleotides to generate more diversity ligase binds D and J segment process similar when DJ segment comes together with V segment to form VDJ as the new segment
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Term
| Relation of antigen to somatic recombination of genes for lymphocytes |
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Definition
| No relation. It is a completely antigen independt process at the DNA level |
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Term
| Role of recombination signal sequences in somatic recombination |
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Definition
| conserved motifs that will flank the minigene segments |
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Term
| During somatic recombination, what mini gene segments do the hypervariable regions tend to be found in |
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Definition
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Term
| What gene region correspons with V-DJ junction in minigene segments for recombination? |
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Definition
CDR (most diverse region associated with Ag binding) |
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Term
| What clinical use are the signal joints within the hairpin loops structures? |
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Definition
| can look at the signal joints and test to see if new lymphocytes are being made in the body |
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Term
Example of how antigen rearrangement can lead to oncogene recombination |
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Definition
could translocate MYC gene from one chromosomne to the chromosome segment with Ig gene this will cause MYC gene to be continually produces, leading to lymphomas or leukemia
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Term
| What makes a good antigen? |
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Definition
protein foreign/dangerous complex structure large can be processed by APC
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Term
Name mini gene segments for light chain? heavy chain? |
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Definition
light chain- V, J, L, C heavy chain- V, D, J, L, C |
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