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30
Medical
Graduate
08/09/2011

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Term
? and ? specifically inhibit NADH dehydrogenase; therefore oxidation of only NADH linked substrates is inhibited.
Definition
Rotenone and Amytal
Term
Cytochrome C oxidase is inhibited by ?, ?, ?.
Definition
CN, azide, CO
Term
?, ?, Cyt a, Cyt a3 undergo oxidation and reduction.
Definition
CuA, CuB,
Term
Cytochrome bc1 complex is blocked by ? ?
Definition
antimycin A
Term
The rate of electron transfer is coupled to and dependent on ?, ?, ?. This is called ? ?.
Definition
ADP, Pi, O2
respiratory control
Term
The energy from oxidation of NADH and FADH2 is captured by the creation of a ? ? ?, which provides the energy for the synthesis of ?
Definition
membrane electrochemical gradient
ATP
Term
During electron transport, the three membrane spanning etc complexes (NADH dehydrogenase, cytochrome bc1, cytochrome oxidase) create a ? ? and membrane potential by pumping protons from the ? side of the inner membrane to the intermembrane side.
Definition
pH gradient
matrix
Term
The intermembrane side becomes more ? and more positively charged.
Definition
acidic
Term
Essence of ? ?: Oxidation and ATP synthesis are coupled by a transmembrane proton gradient
Definition
Oxidative Phosphorylation
Term
The ATP Synthase uses the energy from the proton gradient to ? ?.
Definition
synthesize ATP
Term
The ATPase complex is inhibited by ?, an inhibitor of oxidative phosphorylation binding to F0
Definition
oligomycin
Term
The electron carriers and ATPase only interact through the ? ?.
Definition
electrochemical gradient
Term
How would inhibitors of etc or ATPase affect ATP synthesis? Why?
Definition
Inhibit
Term
Binding of intermembrane H+ by asp in subunit c results in alignment of the subunit a half-channels, and ? ? ? ? ? ? .
Definition
release of protons into the matrix
Term
? spans the inner mitochondrial membrane and generates heat by permitting the influx of H+ into the matrix without the synthesis of ATP
Definition
UCP-1
Term
With NADH linked substrates, electrons pass through ? membrane spanning complexes
Definition
3
Term
ATP molecules/atom oxygen (ATP/O) or P/O = ?
Definition
2.5
Term
With FADH2 linked substrates, electrons pass through 2 membrane spanning complexes
P/O = ?
Definition
1.5
Term
The P/O for malate is ?
Definition
2.5
Term
The P/O for glycerol 3-phosphate is ?
Definition
1.5
Term
? ? is due to uncoupling protein in brown adipose tissue of hibernating animals.
Definition
heat formation
Term
There are inner membrane transporters for ? ?, phosphate, pyruvate, dicarboxylic and tricarboxylic acids, amino acids
Definition
adenine nucleotides (ADP/ATP)
Term
There are no inner membrane transporters for
Definition
NADH, OAA, Acetyl CoA
Term
During glycolysis, what enzyme produces NADH?
Definition
Glyceraldehyde 3-phosphate dehydrogenase
Term
During anaerobic glycolysis, what enzyme re-oxidizes NADH?
Definition
Lactate dehydrogenase
Term
? is the product of aerobic glycolysis.
Definition
Pyruvate
Term
During aerobic glycolysis, cytosolic NADH is reoxidized by ? shuttles, which transfer electrons to the electron transport chain thereby producing energy by ? ?.
Definition
two
oxidative phosphorylation
Term
Malate-aspartate shuttle and glycerol 3-phosphate shuttle only function during ? ?.
Definition
aerobic conditions
Term
Net yield per mole of glucose with glycerol 3-phosphate shuttle
Definition
30
Term
Net yield per mole of glucose with malate-aspartate shuttle
Definition
32
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