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Histology/Staining Methods
Lecture One
44
Biology
Graduate
08/30/2015

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Term
What is Histology?
Definition
The study of cell and tissue structure and the arrangement of the tissues within the organs
Term
Histo means?
Definition
Tissue or Web
Term
List the 7 steps when preparing paraffin sections.
Definition
1. obtain a small tissue sample
2. Fixation
3. Dehydration
4. Clearing
5. Infiltration
6. Embedding
7. Trimming
Term
Describe Fixation
Definition
To preserve cell and tissue structure
Term
Describe Dehydration
Definition
to remove all water
Term
Describe Clearing
Definition
Alcohol is removed by other agents in which both alcohol and paraffin are miscible
Term
Describe Infiltration
Definition
Paraffin permeates the sample
Term
Describe Embedding
Definition
To harden the sample
Term
Describe Trimming
Definition
To expose the sample
Term
What is the name of the device that you make paraffin sections with?
Definition
A microtome
Term
Which staining method stains acidic structures?
Definition
Hematoxylin
Term
Which staining method stains basic structures?
Definition
Eosin
Term
Which staining method stains blue?
Definition
Hematoxylin
Term
Which staining method stains pink?
Definition
Eosin
Term
Which part of the cell stains blue?
Definition
Nucleus and other acidic structures such as RNA rich portions of cytoplasm
Term
Which part of the cell stains pink?
Definition
The cytoplasm
Term
Which staining method distinguishes extracellular tissue components better than H&E?
Definition
Masson's Trichrome
Term
What is the process by which a less specialized cell develops or matures to possess a more distinct structure and function?
Definition
Cell Differentiation
Term
Name the 3 parts that make up the plasma membrane.
Definition
Lipids
Proteins
Oligosaccharide chains
Term
What would you call a lipid that is amphipathic and is bilayered?
Definition
A Phospholipid
Term
Name two type of Plasma Membrane Proteins
Definition
Integral
Peripheral
Term
List the 4 functions of the plasma membrane.
Definition
1. Physical Barrier
2. Selective Permeability
3. Electrochemical Gradients
4. Communication
Term
What is the function of the physical barrier to the plasma membrane?
Definition
Establishes a flexible boundary, protects cellular components and supports cell structure.
Term
What is the function of selective permeability?
Definition
Regulates entry and exit of ions, nutrients, and waste molecules through the membrane
Term
What is the function of electrochemical gradients?
Definition
Establishes and maintains an electrical charge difference across the plasma membrane
Term
What is the function communication in the plasma membrane?
Definition
Contains receptors that recognize and respond to molecular signals
Term
What happens during simple diffusion?
Definition
Lipid and lipid-soluble molecules, such as ethanol and gas, pass freely through the plasma membrane
Term
What happens in Facilitated Diffusion?
Definition
Larger hydrophilic molecules, such as water, glucose and amino acids, require the presence of specific transport proteins (channel or carrier proteins)
Term
Which types of transport requires ATP?
Definition
Active and Vesicular/Bulk transport
Term
Give an example of ions that are actively transported.
Definition
Na/K
Term
Give an example of Vesicular or Bulk transport.
Definition
Endocytosis or Exocytosis
Term
A process in which a substance is ingested into a cell without passing through the cell membrane is called?
Definition
Endocytosis
Term
In which manner of endocytosis involves the uptake of specific macromolecules (hormones) ?
Definition
Receptor-mediated endocytosis
Term
In which manner of endocytosis involves the uptake of bacteria or viruses?
Definition
Phagocytosis
Term
Phagocytosis involves what kind of cell?
Definition
Phagocytes
Term
The non-specific manner of endocytosis that involves the uptake of small volumes of extracellular fluid is called?
Definition
Pinocytosis
Term
Name 3 Cytoplasmic organelles that are involved in protein synthesis.
Definition
Ribosome
Endoplasmic Reticulum
Golgi Apparatus
Term
A globular structure in which two subunits of rRNA and protein acts as the translation site of protein synthesis is called?
Definition
A ribosome
Term
Which organelle is responsible for protein synthesis (producing, transporting and sorting)?
Definition
The rER
Term
Which organelle is responsible for the synthesis of cholesterol based lipids, detoxification of drugs and poisons and sequestration of Ca?
Definition
The sEr
Term
Which part of the ER does not contain ribosomes?
Definition
The sER
Term
Which organelle is a specifically oriented cluster of vesicles, tubules and fattened membrane bound sacs?
Definition
The Golgi apparatus
Term
Which region of the Golgi is the receiving end and which end is the shipping end?
Definition
The cis Golgi receives and the trans Golgi ships
Term
The process that involves the final assembly and glycosylation of proteins and also involves the dispatch to their ultimate destinations is called?
Definition
Protein Synthesis
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