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Histology 1
histology test 1
34
Biology
Not Applicable
08/27/2007

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Term
basophilia staining
Definition
tissue stains more readily with basic dies; materials that are acidic and thus negatively charged, get stained with the positive charged hematoxylin
Term
acidophilia staining
Definition
tissues stain better with acid dyes; the material being died is basic bind to negatively charged Eosin!
Term
metachromasia
Definition
change in color of dye when reacts with certain tissue constituents.
Term
H & E stain
Definition
blue: nucleus, acidic regions of cytoplasm

pink: basic regions of cytoplasm, collagen
Term
Sudan black staining
Definition
stains lipids black
Term
Periodic acid-Schiff (PAS)
Definition
neutral carbohydrates and glycoproteins stain magenta

used to show glycogen, basement membrane and mucus
Term
Alcian blue
Definition
acidic carbohydrates stain blue
Term
Silver
Definition
shows fine structures such as nerves or reticular fibers

stains black
Term
Osmium
Definition
stains lipid containing structures black
Term
Trichrome stains
Definition
nuclei stains dark blue

keratin, muscle and cytoplasm stain red

collagen stains blue
Term
counterstains
Definition
results in intense coloration of cells and tissues
shows and highlights landmarks
Term
Seven steps of tissue processing?
Definition
Fixation, Decalcification, Dehydration, Clearing (replace alcohol with non polar solvent so that non polar paraffin embedding can stick), Embedding, sectioning,Rehydration
Term
polarization microscopy
Definition
light striking oriented molecules rotate and contrast specimen against dark background
Term
phase contrast microscopy
Definition
living cells and tissue; dyes not used;

light changing speed and direction as light passes through differnet structures with different refactive indicis. contrast good but resolution bad
Term
dark field microscopy
Definition
light rays twoard specimen at angle and those rays that hit specimen are reflected through ocular. used for bacteria.
Term
brightfield microscopy
Definition
thin transilluminated specimen, requres the use of dyes and thin sections
Term
fluorescense microscopy
Definition
specimen is stained with fluorescent dye followed by uv light. dye shifts wavelenght of uv light and allows certain fluorochrome stained compounds to be visualized
Term
simple squamous epithelia
Definition
single layer, flat
includes mesothelium (creates lubricant) and endothelium (lines blood vessels and body cavities and also has clotting factors)
Term
simple cuboidal epithelia
Definition
round nucleus, cube shaped
Term
simple columna epithelia
Definition
nuclei is either in the middle of cell or at the base;
tall elongated nucleus;
gun lining, lubricating function
***the only columnar with nucleus in apex is ameloblasts!
Term
stratified epithelia
Definition
surface layer of cells its the name for the entire epithelia

if surface is squamous, then the epithelia is named stratified squamous
Term
transitional epithelium
Definition
urinary tract, specialized for stretching, empty vs full bladder
Term
merocrine
Definition
method of secretion with membrane bound vesicles containing secretory product, fuses with cell membrane and is secreted leaving cell intact
Term
apocrine secretion
Definition
loss of apical portion of cytoplasm which encloses the secretory product
Term
holocrine secretion
Definition
degeneration and discharge of entire cell (sebacious gland), the whole cell breaks down and everything is released
Term
serous cells
Definition
surround narrow lumen; nucleus is rounded at base; cell borders are poorly defined and very indistinct
Term
mucous cells
Definition
surround wide lumen, nucleus flattened at base of cell, cell boundaries sharply defined
Term
mix cell type
Definition
both serous and mucous cells;
demilune moon shaped-secretes serous products into mucous acinus forming mixed secretion
Term
satellite cells
Definition
supporting cells that surround nerve cell bodies that protect hte neuron, provide electrical insulation and exchange metabolites.
Term
tactile corpuscle of Meissner
Definition
tactile nerve endings; found in finger tips, palms, soles
Term
pacinian corpuscle
Definition
encapsulated
responds to deep pressure and vibration
Term
corpuscle of ruffini
Definition
encapsulated
detection of position, movement, sliding motion and touch
Term
end bulbs of krause
Definition
encapsulated
thermoreceptor?
unknown function as of now!!!
Term
Merkel's corpuscle
Definition
non encapsulated afferent
receptor for delicate touch, associated with modified epithelial cells
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