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Heme 2nd Lab Practical
WBC
70
Biology
Undergraduate 3
10/29/2014

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Term
ALL stands for
Definition
Acute Lymphoblastic Leukemia
Term
CLL stands for
Definition
Chronic Lympocytic Leukemia
Term
AML stands for
Definition
Acute Myeloid Leukemia
Term
CML stands for
Definition
Chronic Myelogenous Leukemia
Term
CML Platelets
Definition
Abnormal number and morphology
Term
CML Eosinophils and Basophils
Definition
Increased with immature forms
Term
Leukemoid Reaction Platelets
Definition
Normal
Term
Leukemoid Reaction Eosinophils and Basophils
Definition
Normal
Term
CML Dyspoiesis
Definition
Present
Term
Leukemoid Reaction Dyspoiesis
Definition
Absent, but may be reactive changes
Term
CML Leukocyte alkaline phosphatase
Definition
Markedly decreased
Term
Leukemoid Reaction Alkaline phosphatase
Definition
Increased
Term
CML Neutrophils
Definition
Increased with immature cells; peaks at myelocytes and segmented neutrophil stages
Term
Leukemoid Reaction Alkaline phosphatase
Definition
Increased with immature forms; orderly progression of maturation stages with no peaks
Term
Toxic granulation is associated with
Definition
Bacterial infections, sepsis, following administration of granulocyte colony-stimulating factor
Term
Dohle body associated with
Definition
Bacterial infection, sepsis, pregnancy
Term
Hypersegmentation associated with
Definition
Megaloblastic anemia; chronic infections; myelodysplastic syndrome; rarely inherited
Term
Reactive lymphs are associated with
Definition
Viral infections and other antigenic stimulation, including organ transplantation
Term
Flame cells are associated with
Definition
Plasma cells that are producing IgA
Term
T or F: CLL is broken down into 3 stages
Definition
True
Term
What are the stages of ALL?
Definition
L1
L2
L3
Term
AML is broken down into how many stages?
Definition
7
Term
CLL is composed mainly of ____ ____
Definition
Mature lymphocytes
Term
ALL is composed mainly of ____
Definition
Lympoblasts
Term
CML is composed mainly of immature ____ and _____, and _____.
Definition
basophils
eosinophils
Micromegakaryocytes
Term
CML has a spectrum of myeloid cells with a predominance of ____ & ____ _____
Definition
Myelocytes
Segmented Neutrophils
Term
CML Myeloblasts _____ 5%
Definition
<
Less Than
Term
CML parameters:
WBC
RBC
Platelets
Definition
Increased
Normal of decreased
Normal or increased
Term
Polycythemia Vera Parameters:
WBC
RBC
Platelets
Definition
Normal or increased
Increased
Normal or increased
Term
Essential Thrombocythemia Parameters:
WBC
RBC
Platelets
Definition
Normal or slightly increased
Normal or slightly decreased
Increased
Term
Primary myelofibrosis parameters:
WBC
RBC
Platelets
Definition
Normal, increased, or decreased
Normal or decreased
Normal, increased, or decreased
Term
What will you find in the peripheral blood of Primary myelofibrosis
Definition
Teardrop cells
Nucleated RBC
Polychromasia
Giant atypical shaped platelets
abnormal granulation in platelets
circulating micromegakaryocytes
Term
CLL BM & Peripheral is full of
Definition
small mature lymphocytes
Term
MM is a
Definition
malignant proliferation of plasma cells in BM
Term
T or F: Rouleaux is often seen in MM
Definition
True
Term
Ballerina skirt is often associated with
Definition
Reactive Lymphocyte
Term
T or F: vacuoles may be seen in the cytoplasm of reactive lymphocytes
Definition
True
Term
Myeloblasts
Auer rods
sheet or blasts
Definition
AML M1
Term
Myeloblasts
Promyelocytes
Auer rods
Definition
AML M2
Term
Promyelocytes
hypergranulation
Nuclei kidney bean shaped
Auer rods
Definition
AML M3
Term
Myeloblasts
Monoblasts
Definition
AML M4
Term
Monoblasts
Promonocytes
Monocytes
Definition
AML M5
Term
Nucleated RBC
Abnormal RBC precursors
Immature RBC precursors
Definition
AML M6
Term
Megakaryoblasts
Micromegakaryocytes
Blasts shedding platelets
Definition
AML M7
Term
CML marked increase in proportion of
Definition
band neutrophils
Term
CML BM will be
Definition
hypercellular with NO fat cells
Term
MPX detects the presence of _____ in myeloid cells
Definition
granules
Term
SB detects ___ ____
Definition
intracellular lipids
Term
SB will Positively stain _____ and negatively stain _____
Definition
Myeloblasts
Monoblasts
Term
T or F: Many auer rods that cannot be seen with Wright's stain can be seen with MPX
Definition
True
Term
SB will also positively stain ____ and negatively stain ______ and ______
Definition
myelocyte
lymphoblasts
lymphocytes
Term
NSE will positively stain
Definition
moocytic lineage
Term
Chloroacetate esterase (blue-black) will positively stain
Definition
myeloblasts
Term
Butyrate esterase (yellow-brown) will positively stain
Definition
monoblasts
Term
PAS detects the presence of
Definition
intracellular glycogen
Term
PAS positively identifies ____, _____, & ____
Definition
M6
M7
ALL
Term
PAS negative in ____ - ____
Definition
M1
M5
Term
Normal LAP
Definition
15-100
Term
LAP score will be _____ in leukemoid reactions and ____ in CML
Definition
High
Low
Term
LAP will also be high in ______ & _____
Definition
PV
Pregnancy
Term
LAP: segs and bands are devoid of enzyme. This correlates with
Definition
CML
Term
Normal WBC count
Definition
4.0-11.0 x 10^3 / microliter
Term
Normal Platelet count
Definition
150-450 x 10^3 / microliter
Term
Normal RBC count
W
M
C
Definition
4.2-5.4 x 10^6 / microliter
4.7-6.1 x 10^6 / microliter
4.4-5.8 x 10^6 / microliter
Term
Normal reticulocyte %
Definition
0.5-1.5%
Term
Normal Hgb Count
M
W
C
N
Definition
14-18
12-15
17-23
10-15
Term
Normal Hct Count
M
W
C
Definition
40-54
35-49
48-68
Term
Normal ESR
M
W&C
Definition
0-15
0-20
Term
how do you calculate platelets?
Definition
(# counted/5 fields) x 20,000
Term
how do you calculate WBC estimation
Definition
(# counted/5 fields) x 2,000
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