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Genetics: Storage and Expression of Genetic Info
Storage and Expression of Genetic Info
48
Biology
Undergraduate 4
04/22/2010

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Term
Genetic Code
Definition
a specific sequence of ribonucleotide bases found in mRNA that specifies each amino acid.
Term
Protein
Definition
Polymers of the 20 amino acids.
Term
Codon
Definition
Three nucleotide bases.
Term
Unambiguous
Definition
Each dodon specifies only one amino acid.
Term
Degenerate/Redundant
Definition
Many amino acids are specified by more than one codon.
Term
Ordered
Definition
Most redundant codons for the same amino acid are the same except at the third codon position.
Term
Nonoverlapping
Definition
Each base is part of only one codon. Each base is only read once during translation.
Term
Universal
Definition
with a few minor exceptions, the same code is used in all living things.
Term
Cirkc's Wobble Hypthesis
Definition
The 5' base on the anticodon, which binds to the 3' base on the mRNA, is not as spatially confined as the other two bases, and could thus have non-standard base pairing.
Term
Inosine
Definition
Modified base found only in tRNA can pair with C,U, or A
Term
Start Codon
Definition
AUG and very rarely GUG.
Term
Stop Codons
Definition
UAA, UAG, UGA
Term
Nonsense Mutation
Definition
Mutation from a normal codon to a termination codon.
Term
Supressor mutations
Definition
Conversion of stop codon to amino acid encoding codon.
Term
Template Strand and Partner Strand
Definition
Two strads of DNA that act as a template in forming a single-stranded mRNA molecule.
Term
RNA Polymerase
Definition
Holoenzyme consisting of several enzymes working together. Carries out transcription from 3' to 5' along the template DNA adding nucleotides to the 3' end of mRNA.
Term
Promoters
Definition
sequences of DNA that are the points of attachment for RNA polymerase
Term
Pribnow Box
Definition
TATAAT. Bacteria
Term
Location of Promoters
Definition
About 10 bases upstream or toward the 3' end of the gene to be transcribed.
Term
Major Promoters in Prokaryotes (3)
Definition
TATA, Pribnow, TTGACA
Term
Sigma Subunit of RNA Polymerase
Definition
Recognizes and binds to the promoter.
Term
Beta and Beta' Subunits of RNA Polymerase
Definition
Perform the actual transcription.
Term
5' to 3'
Definition
Direction mRNA is built in transcription.
Term
3' to 5'
Definition
Direction template DNA strand is read in transcription.
Term
Polycistronic messages.
Definition
Related genes are contiguous and transcribed as a unit. Rare in eukaryotes
Term
Overlapping genes in virus ФX174
Definition
Multiple initiation codons out of frame.
Term
RNA Polymerases I, II, III.
Definition
Three types of RNA polymerase used in Eukaryotic transcription.
Term
Posttranscriptional Modifications in Eukaryotic Transcription (2)
Definition
Splicing and Caps & Tails.
Term
Polymerase II
Definition
Transcribes all mRNA
Term
Cis-Acting Elements
Definition
occur near (promoter region + enhancers) (next to) the same DNA molecule as the gene being transcribed
Term
Trans-Acting Elements
Definition
occur further away or on different chromosomes (Transcription factors (proteins))
Term
TATA Box
Definition
seven nucleotide long element about -35 (or upstream) of gene.
Term
CAAT Box
Definition
- upstream of TATA box - increases transcriptional efficiency (-80) Eukaryotes
Term
Enhancers
Definition
position can vary but are necessary for maximally efficient transcription
Term
hnRNA
Definition
Heterogeneous nuclear RNA. Raw mRNA transcripts.
Term
hnRNP
Definition
Heterogeneous nuclear ribonucleoproteins. Complex of hnRNA and proteins.
Term
7'methylguanosine (7mg) and function (2)
Definition
Cap added to 5' end of hnRNA. Provides protection from nucleases and transports into cytoplasm.
Term
Poly-A Tails
Definition
Added to 3' end of hnRNA. Last 10-35 ribonucleotides removed. AAUAAA.
Term
Exons
Definition
Expressed parts of genes.
Term
Introns
Definition
Useless segements of genes that aren't expressed. Are spliced out of hnRNA to form mature mRNA.
Term
Spliceosome
Definition
Enzyme that splices introns out of hnRNA to form mRNA.
Term
Ribozymes
Definition
Self-excising introns
Term
Group I Ribozyme
Definition
rRNAs
Term
Group II Ribozyme
Definition
MT and CP mRNA and tRNA
Term
snRNP
Definition
Small nuclear ribonucleoproteins. Uracil rich. Components bind to intron recognition sites.
Term
Lariat structure
Definition
Twisted mRNA due to snRNPs.
Term
Substitution Editing
Definition
Nucleotides are changed.
Term
Insertion/Deletion Editing
Definition
Total number of nucleotides is increased or decreased.
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