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Genetics Leftovers
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63
Biology
Undergraduate 3
12/16/2010

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Term
sex limited
Definition
characteristic expressed in one sex and not the other even if both sexes have the genotype
Term
sex influenced
Definition
characteristic expressed more frequently in one sex over the other
Term
radiomimetic
Definition
chemical that simulates radiation
Term
restriction fragment length polymorphism
Definition
genetic analysis using broken dna and electrophoresis
Term
variable number tandem repeats
Definition
genetic analysis that looks at the unique pepetitive DNA that is different in every person
Term
fluorescence activated chromosome sorting
Definition
genetic analysis that isolates human chromosomes and can introduce it to other species to see if it's expressed
Term
radiation hybrid mapping
Definition
irradiation cells and fragmenting dna to observe linkage
Term
southern blotting
Definition
genetic analysis using electrophoresis and probe hybridization
Term
clone
Definition
exact replica of a biological entity
Term
DNA clone
Definition
multiple copies of a single DNA sequence
Term
vector
Definition
DNA cloning vehicle which is it self DNA
Term
transformation
Definition
process of introducing recombinant plasmids to the new host throught their cell walls
Term
LacZ
Definition
determines ability of gene to grow
Term
lambda, pBR322, pUC18
Definition
three popular cloning vehicles
Term
M12 phage
Definition
single stranded DNA phage
Term
cosmids
Definition
hybrid plasmid used as a vector with cohesive sites from lambda phage
Term
bacteria artificial chromosomes BACs
Definition
cloning vehicles based on e coli F factor
Term
Yeast artificial chromosomes YACs
Definition
linear vectors with yeast telomere ends
Term
expression vectors
Definition
uses to allow bacteria to produce foreign DNA gene products eg insulin
Term
Polymerase Chain Reaction
Definition
a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
Term
Taq polymerase
Definition
heat tolerant enzyme that catalyzes polymerase chain reaction
Term
agrobacterium
Definition
well known for its ability to transfer DNA between itself and plants; made to produce BT (pesticide)
Term
shuttle vector
Definition
vector that grows well in two different species of host;
Term
retrovirus
Definition
RNA virus cloing vehicle
Term
shotgun cloning
Definition
cloning entire genome at once
Term
in vitro amplification
Definition
polymerase chain reaction is a type of
Term
transgenic organism
Definition
organism with cells made up of foreign DNA usually for commercial purposes
Term
same person that receives benefit should receive the risk
Definition
gene therapy hippocratic oath
Term
genomics
Definition
a discipline in genetics concerning the study of the genomes of organisms
Term
structural genomics
Definition
characterizing the physical nature of the genome
Term
functional genomics
Definition
characterizing the transcripts and proteins the genome produces
Term
aporepressor
Definition
corepressor adds to the _______ to turn off operon
Term
positive, cAMP-CAP, negative, inducer, repressor
Definition
lac operon operates either with the ______ regulation using ______ or the ________regulation where lactose, the _______ binds to the ______
Term
beta galactosidase
Definition
protein encoded in lac operon that breaks down lactose into sugars for metabolism
Term
permease
Definition
lets lactose into e coli more easily
Term
gene pool
Definition
sum of all alleles in a population at a given time
Term
allele frequency
Definition
proportions of given alleles
Term
population genetics
Definition
analyzing allele frequency over time
Term
p^2 + 2pq + q^2
Definition
hardy weinberg principle
Term
fitness, 1-s (selection coefficient)
Definition
W=
Term
-sq2
Definition
selection coefficient p2+2pq+q2_____
Term
Sewall Wright, inbreeding coefficient
Definition
population geneticist that thought of F, _____
Term
inbreeding
Definition
increase homozygosity in a population
Term
selection
Definition
kills homozygosity in a population
Term
inbreeding
Definition
reduces fitness
Term
crossbreeding
Definition
increases fitness
Term
hybrid vigor
Definition
improved health of heterozygote; heterosis
Term
less deleterious recessives and overdominance
Definition
2 reasons for hybrid vigor
Term
evolution
Definition
central paradigm for modern biology
Term
speciation
Definition
gene pool is separated and changes from one another
Term
(a+b)^2n
Definition
n gene pairs binomial equation
Term
meristic liability threshold
Definition
memorize these three words they may come in handy
Term
Sutton-Boveri 1903
Definition
disc. genes on chromosomes
Term
Lyon Hypothesis
Definition
hypothesized barr body = inactivated x
Term
Morgan
Definition
disc. gene pairs inherited together, separation of genes via crossing over
Term
Creighton and McClintock
Definition
saw/disc. crossing over
Term
Frederick Griffith
Definition
his experiment discovered transformation
Term
Avery, MacLeod, McCarty
Definition
their experiment showed that DNA was the transforming principle
Term
Hershey-Chase experiment
Definition
found that DNA not protein was genetic material
Term
Meselson-Stahl experiment
Definition
experiment that disproved conservative and dispersive models
Term
Arthur Kornberg
Definition
studied ingredients for DNA replication
Term
Cairns
Definition
used heavy hydridine to enlarge DNA and see two replicating forks
Term
Beadle and Tatum
Definition
one gene - one enzyme hypothesis/experiment
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