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Genetics Exam 4
Genetics terms
38
Biology
Undergraduate 2
11/08/2015

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Term
Conjugation
Definition
Genetic transfer and recombination where physical contact is needed
Term
Plasmid
Definition
small DNA molecule that is separate from the chromosomal DNA. Usually found in bacteria as a round, double stranded structure.
Term
Rolling Circle
Definition
Term
HFR
Definition
Bacteria with conjugative plasmid in it's DNA
Term
Interrupted Mating
Definition
Used to map bacterial genes by determining the order donor genes entered the recipient cell
Term
Transformation
Definition
Turning one genotype into another by introducing outside DNA
Term
Transduction
Definition
Moving of genes from one bacteria to another by a phage
Term
Lytic
Definition
Viral reproduction where the infected cell is destoryed
Term
Plaque
Definition
Term
Recombination
Definition
Creates new DNA by combing two separate DNA
Term
Phage
Definition
Term
Recombinant DNA
Definition
New DNA formed from two originally separate DNA
Term
Restriction Endonuclease
Definition
Cut DNA at a specific place
Term
Plasmid
Definition
Autonomously replicating DNA molecule
Term
Cloning Vector
Definition
Plasmid or phage used to carry the cloned DNA segement
Term
Chimera
Definition
Recombinant DNA
Term
Library
Definition
Collection of clones that span the target DNA
Term
cDNA
Definition
Complementary DNA;
Term
Screening
Definition
Testing for genetic diseases
Term
DNA Sequencing
Definition
Process that determines the precise order of nucleotides on DNA
Term
Northern Blot
Definition
Used to study gene expression by detecting RNA
Term
Southern Blot
Definition
DNA fragments are separated on a gel and is transferred to membrane. Membrane is probed and the pattern reflects the bands.
Term
Hybrdization
Definition
Combing to two complementary DNA strands to form single double-stranded molecule
Term
Electrophoresis
Definition
Separates DNA based on size. Smaller pieces move faster and larger slower.
Term
Probe
Definition
Mark the clone carrying the certain gene
Term
Restriction Mapping
Definition
Using restriction enzymes to map the structure of DNA
Term
PCR
Definition
Technique to copy DNA
Term
Genome
Definition
DNA content of a single cell
Term
Mini/Microsatellite
Definition
Highly variable regions on DNA used for DNA fingerprinting
Term
SNP
Definition
Single base substitution that is polymorphic in the population. Can be used to identify linkage
Term
RFLP
Definition
Restriction Fragment Length Polymorphisms. Detects SNP
Term
Transcriptome
Definition
All the transcripts present in a given cell. Can get RNA to bind to DNA
Term
Proteome
Definition
Complete complement of proteins
Term
Interactome
Definition
How proteins, RNA, DNA, etc interact and bind
Term
DNA Microarrays
Definition
DNA samples bound to a chip. Show what genes are expressed
Term
Genomics
Definition
Applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes
Term
Lysogenic
Definition
Viral reproduction where the virus integrates into the host cell
Term
Western blot
Definition
Detects protiens
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