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Genetics Exam III
N/A
92
Biology
Undergraduate 4
10/18/2011

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Term
Shigella
Definition
a medical condition caused by shigella dysenteriae and highly associated with poverty and developing countries
Term
Shigella dysenteriae
Definition
a bacterium that forms SLT toxins alongside EHEC 0157:H7
Term
Genetic recombination
Definition
replacement of one or more genes present in one strain of bacteria with those from a genetically distinct strain.
Term
Rec A protein
Definition
promotes genetic exchange between a fragment of donor and recipient DNA
Term
Single strand displacement
Definition
common form of recombination in many bacterial species
Term
Rec BCD protein
Definition
a protein that unwinds the DNA helix and one strand is often degraded
Term
Microangiopathic hemolytic anemia
Definition
anemia with the presence of fragmented erythrocytes
Term
Hematuria
Definition
bloody urine
Term
Thrombocytopenia
Definition
low platelet counts (less than 100 thousand)
Term
Oliguria
Definition
low urine output
Term
Resistance Transfer Factor (RTF)
Definition
component of R-plasmid that encodes information that is important in aiding the donor bacteria transferring the plasmid to the recipient bacteria
Term
r-determinants
Definition
second component of R-plasmid that is the segment that encodes genetic information regarding antibiotics resistance
Term
conjunction
Definition
method of transfer that is unidirectional through physical contact between two genetically unrelated bacteria
Term
transformation
Definition
method of transfer involving a prokaryote picking up genetic info from surrounding environment
Term
transduction
Definition
genetic transfer involving a prokaryote acquiring new genetic info through bacteriophage infection; and involves lambdoid bacteriophages
Term
col plasmid
Definition
transferrable plasmid that encodes toxic proteins who are to target neighboring bacteria thereby allowing bacterium to eliminate their competition
Term
Colicinogenic
Definition
the bacterium that contain col plasmid in order to eliminate neighboring bacteria
Term
Lysogenic
Definition
the semi-dormant phase of the viral lifecycle where the incorporation of the viral genetic material occurs
Term
Prophage
Definition
a transformed viral infected bacteria that will live out its normal life with a newly incorporated viral genetic sequence as well as producing progeny
Term
Lytic
Definition
the phase in which the bacteria will become a viral factory concentrating its cellular function on producing more viruses
Term
Competent
Definition
bacteria that are capable of naturally or artificially forced to take up new genetic materials
Term
OriC
Definition
the promoter sequence
Term
Competence proteins
Definition
proteins/enzymes designed to make certain that the DNA acquired is capable of being accepted
Term
DNA translocase
Definition
that which takes the new genetic sequence into the cell if similarities in sequences exist
Term
Rec A protein
Definition
newly acquired DNA sequence is incorporated into the homologous region of the bacterium DNA
Term
Attachment
Definition
initial stage of viral infection of a cell, recognition of host cell
Term
Cellular recognition proteins
Definition
this is the site species-specific viruses recognize and attach to of a host cell before proceeding with the next step
Term
Penetration
Definition
second step of viral infection; this is the insertion of the viral genetic material
Term
Fusion
Definition
a method of penetration involving the merge/union between viral envelope and host cellular membrane whereby the entire virus enters host cell
Term
Translocation
Definition
virus induces the formation of a channel protein through the host cellular membrane
Term
Endocytosis
Definition
induces the host cell to absorb the entire viral particle
Term
Insertion
Definition
third step of viral infection; the genetic material is inserted into the host cell’s DNA
Term
Lytic
Definition
active phase, production of viral particles
Term
Lysogenic conversion
Definition
the manner that the virus incorporates its genetic information into a host cell’s genome
Term
Prophage
Definition
an infected host cell that immediately proceeds into the dormant phase
Term
Biosynthesis
Definition
fourth step of viral infection which involves the active phase of viral particle production, inactivates host genes not necessary for reproduction and begins replication and synthesis of new proteins for the viral capsid
Term
Maturation
Definition
fifth step of viral infection involves assembly of newly produced viruses within host cells
Term
Release
Definition
sixth step of viral infection; discharges viruses into the surrounding environment
Term
Generalized transduction
Definition
the entire viral DNA is excluded while a large amount of bacterial chromosome is packaged into the virus
Term
Abortive transduction
Definition
this form of viral injected bacterial DNA do not replicate but is transmitted to one progeny following each division. Remain in the cytoplasm
Term
Complete transduction
Definition
a partially diploid bacteria that is produced with the ability to replicate because the genetic sequence combines with the homologous regions of the new host’s bacterial chromosome
Term
Specialized transduction
Definition
small piece of the bacterial DNA is packaged along with the virus prior to dissemination of the virion. Only a few bacterial genes are packaged within the virus
Term
Auxotroph
Definition
mutated bacteria that has lost the ability to synthesize one or more organic components
Term
Prototrophs
Definition
wild type bacteria that can survive and synthesize all materials need in a minimal medium
Term
Bacterial conjunction
Definition
method of unidirectional transfer of genetic materials through direct physical contact between two genetically unrelated bacteria
Term
Episome
Definition
autonomously replicating plasmids that found in unidirectional transfer, or bacterial conjunction
Term
Donor bacterium
Definition
a type of bacterium F+ cell that is responsible for transferring the genetic materials
Term
Recipient bacterium
Definition
F- cell; a bacterium that receives the genetic material
Term
F-factor
Definition
a small autonomously replicating plasmid and has the ability to form a sex pilus; the bacterium that is responsible for transferring the genetic materials possesses this
Term
Sex pilus
Definition
conduit for transfer, sometimes formed by a bacterium responsible for transferring genetic materials
Term
Autonomous state
Definition
the autonomously replicating plasmid (episome) exists unassociated plasmid located in the cytoplasm
Term
Integrated state
Definition
the autonomously replicating plasmid (episome) which is incorporated into the bacterial chromosome
Term
Hfr cell
Definition
a bacterium that possesses an incorporated plasmid
Term
Diploid
Definition
produced by plasmids who can transfer the entire bacterial chromosome from bacterium to the next
Term
F-pilus
Definition
flexable tube known to bring the two bacteria closer together but is not responsible for transferring genetic information. Connects the F+ and F- bacterium
Term
Mating pair formation
Definition
the establishment of intimate physical contact between a F+ and F-cell
Term
Conjugation channel
Definition
a channel that allows plasmid to be transferred between F+ and F-
Term
Internal sex filter
Definition
the place to allow only the transfer of plasmid and prevent the entire cellular content from transferring between the F cells
Term
Enterohemorrhagic disease
Definition
caused by EHEC/0157:H7 or E. coli
Term
A/E lesions
Definition
characterized by the intimate attachment of the bacilli to the apical surfaces enterocytes of the intestines which result in the effacement of the brush border
Term
VT1 and VT2
Definition
also produce SLTs
Term
AB toxins
Definition
group of toxins that consist of two polypeptide parts (ratios 1:5)
Term
A component
Definition
the enzyme
Term
B component
Definition
the binding protein and is essential to make the first component active
Term
GB3
Definition
Component B binds to this receptor found on human endothelial cells and nerve cells
Term
Retrograde transported
Definition
the binding of GB3 initiates endocytosis where Component A this and Golgi apparatus to the ER and nuclear envelope
Term
N-glycosidas
Definition
targets a single adenine residue from 28S ribosomal RNA
Term
Chemokine Interleukin-8
Definition
AB toxins induce its expression
Term
Induction
Definition
The process of turning “on” the expression of genes in response to their environment
Term
Inducible enzymes
Definition
the enzymes produced only when necessary
Term
OriT
Definition
the DNA replication of the self replicating plasmid can be initiated for uni-direction genetic transfer
Term
OriV
Definition
the site of initiation of replication of the self replicating plasmid but is used in cellular fission and not during the autonomously transfer of genetic material
Term
OriS
Definition
site of replication initiation of the self replicating plasmid but is used as a backup when OviT and OviV fail to function
Term
DNA relaxase
Definition
recognizes the locus containing OriT and cleaves it at a specific site called nic which forms a DNA break and thereby allowing the double stranded DNA to be pulled apart
Term
Rolling circle replication
Definition
the type of replication that takes place during genetic transfer
Term
Positive control mechanism
Definition
utilizes certain product of the regulator gene to turn on the expression of one or more structure genes or genes that specify the amino acid sequences of enzymes and other proteins
Term
Negative control mechanism
Definition
utilizes certain product of the regulator gene to turn off the expression of one or more structure genes or genes, that specify the amino acid sequences of enzymes and other proteins
Term
Activator
Definition
product of the regulator gene that is used to turn on expression
Term
Repressor
Definition
product of the regulator gene that is used to turn off the expression
Term
RPBS
Definition
activator or repressor will bind to this which is located next to an area called promoter which contains CpG island and TATA box
Term
Promoter
Definition
area of the DNA which contains sequences such as CpG islands and TATA box
Term
Effector molecules
Definition
OriT depends on the absence or presence of these molecules
Term
Inducer
Definition
molecules that are involved in the promotion of gene expression
Term
Co-repressor
Definition
molecules that are involved in the suppression of gene expression
Term
Allosteric transition
Definition
effector molecules binds to the product of the regulator gene and causes a change in this structure and activates the molecule
Term
Depression
Definition
the 5 genes that encode enzymes for the synthesis of tryptophan is turned on
Term
Lactose
Definition
galactose+glucose medium of disaccharide sugar
Term
Lac operon
Definition
the area of control within the E. coli DNA and transcribes two enzymes
Term
Beta-galactoside permease
Definition
enzyme responsible for transporting disaccharide lac Y gene
Term
Lac Y gene
Definition
disaccharide transported by Beta galactoside permease
Term
Beta-galactosidase
Definition
enzyme responsible for catabolizing the disaccharide into monosaccharide glucose and galactose
Term
Lac Z gene
Definition
genetic sequence that encodes Beta-glactosidase
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